Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F69 I0 R1
|
1426 |
148.0 |
5875178 |
97.4% |
5722423 |
123.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,340,380 |
T→A |
8.1% |
intergenic (+91/+37) |
ubiG → / ← yfaL |
bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,340,380 | 0 | T | A | 8.1%
| 207.0
/ 5.0
| 61 | intergenic (+91/+37) | ubiG/yfaL | bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin |
| Reads supporting (aligned to +/- strand): ref base T (30/26); new base A (2/3); total (32/29) |
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGC > NC_000913/2340244‑2340523
|
ccccGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAACGCCTTATCCg < 3:825510/149‑1 (MQ=38)
aaCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGt > 2:792114/1‑141 (MQ=255)
aaCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGt < 1:792114/141‑1 (MQ=255)
cTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta < 3:1124813/127‑4 (MQ=38)
cTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta > 4:1124813/1‑124 (MQ=38)
tataTGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta < 2:816403/126‑4 (MQ=38)
tataTGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta > 1:816403/1‑123 (MQ=38)
cGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAtt > 4:489631/1‑149 (MQ=255)
aGTGAGGGTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCa > 2:1294057/1‑149 (MQ=255)
tGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAc < 3:489631/149‑1 (MQ=255)
tttGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc < 1:1294057/149‑1 (MQ=255)
gCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa < 3:1106581/115‑1 (MQ=255)
gCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa > 4:1106581/1‑115 (MQ=255)
gcgcCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTc > 3:1036607/1‑149 (MQ=255)
cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa > 2:1071850/1‑88 (MQ=255)
cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa < 1:1071850/88‑1 (MQ=255)
aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGt > 1:1334579/1‑105 (MQ=255)
aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGt < 2:1334579/105‑1 (MQ=255)
ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCTGACTAGACAGTGa > 1:704878/1‑103 (MQ=255)
ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCTGACTAGACAGTGa < 2:704878/103‑1 (MQ=255)
cGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCAc > 4:480473/1‑149 (MQ=255)
gCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc < 1:1290587/118‑1 (MQ=255)
gCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc > 2:1290587/1‑118 (MQ=255)
ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTgg < 1:827921/127‑1 (MQ=255)
ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTgg > 2:827921/1‑127 (MQ=255)
aagcgTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt < 4:1358233/147‑1 (MQ=255)
cgcgTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTg > 2:686632/1‑149 (MQ=255)
ttATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGt > 2:1172600/1‑95 (MQ=255)
ttATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGt < 1:1172600/95‑1 (MQ=255)
tATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTggg > 1:715665/1‑120 (MQ=255)
tATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTggg < 2:715665/120‑1 (MQ=255)
aTCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccacgcca > 2:1443778/1‑149 (MQ=255)
tCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccacgccac > 2:94745/1‑149 (MQ=255)
aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt < 2:875945/82‑1 (MQ=255)
aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt > 1:875945/1‑82 (MQ=255)
tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGa > 4:1283081/1‑64 (MQ=255)
tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGa < 3:1283081/64‑1 (MQ=255)
gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTg > 4:831162/1‑102 (MQ=255)
gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTg < 3:831162/102‑1 (MQ=255)
gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt < 4:729136/125‑1 (MQ=255)
gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt > 3:729136/1‑125 (MQ=255)
cTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGa < 1:686632/149‑1 (MQ=255)
tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGac > 4:444291/1‑149 (MQ=255)
tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGac > 2:224671/1‑149 (MQ=255)
cccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACaaaaa < 3:1370717/86‑1 (MQ=255)
cccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACaaaaa > 4:1370717/1‑86 (MQ=255)
gCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctga < 1:1443778/149‑1 (MQ=255)
ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc > 3:572382/1‑133 (MQ=255)
ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc < 4:572382/133‑1 (MQ=255)
gATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCCCGCCACGCTGCCGCGCAGGGACACTCGctgact > 2:727258/1‑147 (MQ=255)
gATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgactga > 1:639315/1‑149 (MQ=255)
gATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgact < 1:727258/147‑1 (MQ=255)
aaGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGatat > 4:145106/1‑149 (MQ=255)
aGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATAtt < 1:224671/149‑1 (MQ=255)
gCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAat > 4:170455/1‑89 (MQ=255)
gCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAat < 3:170455/89‑1 (MQ=255)
gCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGc < 1:94745/149‑1 (MQ=255)
gTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAatcatc < 1:73610/91‑1 (MQ=255)
gTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAatcatc > 2:73610/1‑91 (MQ=255)
gTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAAtcatca < 2:182482/92‑1 (MQ=255)
gTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAAtcatca > 1:182482/1‑92 (MQ=255)
|
CCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGC > NC_000913/2340244‑2340523
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
