Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F216 I0 R1 247 44.8 1629152 98.0% 1596568 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 940,380 T→C 8.3% L318S (TTG→TCG)  serS → seryl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,3800TC 8.3% 140.4 / 4.1 48L318S (TTG→TCG) serSseryl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (21/23);  new base C (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

CTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACGTTTGGGATTTCCA  >  NC_000913/940233‑940524
                                                                                                                                                   |                                                                                                                                                
cTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTg                                                                                                                                                 >  1:115910/1‑149 (MQ=255)
 tGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGc                                                                                                                                                >  1:205001/1‑149 (MQ=255)
     gCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg                                                                                                                                            >  2:44588/1‑149 (MQ=255)
     gCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg                                                                                                                                            >  1:6388/1‑149 (MQ=255)
     gCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTgg                                                                                                                                             >  3:357600/1‑148 (MQ=255)
        ggTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGcc                                                                                                                                          <  4:357600/148‑1 (MQ=255)
               atGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgta                                                                                                                                   <  4:314935/148‑1 (MQ=255)
                tGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgtacc                                                                                                                                 <  2:380539/149‑1 (MQ=255)
                  gTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCACTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgtaccgt                                                                                                                               >  1:291120/1‑149 (MQ=255)
                     gTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTg                                                                                                                                              >  1:194264/1‑131 (MQ=255)
                     gTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTg                                                                                                                                              <  2:194264/131‑1 (MQ=255)
                          acCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca                                                                                                                        <  3:211192/148‑1 (MQ=255)
                            ccGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatcatc                                                                                                                      >  4:40217/1‑148 (MQ=255)
                            ccGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATcc                                                                                                                     >  2:235990/1‑149 (MQ=255)
                               tGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCtt                                                                                                                   <  3:228168/148‑1 (MQ=255)
                                   cTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCa                                                                                                               >  3:69412/1‑148 (MQ=255)
                                            aTGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACAt                                                                                                     <  1:156782/149‑1 (MQ=255)
                                            aTGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACAt                                                                                                     <  1:186240/149‑1 (MQ=255)
                                                 ccAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt                                                                                                >  2:114937/1‑149 (MQ=255)
                                                   aGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCttt                                                                                              <  1:235990/149‑1 (MQ=255)
                                                            aaGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTg                                                                                      >  3:279672/1‑148 (MQ=255)
                                                               ttGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCaa                                                                                   >  3:265326/1‑148 (MQ=255)
                                                                tGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCaaaa                                                                                 <  2:389936/149‑1 (MQ=255)
                                                                    aTGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTa                                                                             <  2:6388/149‑1 (MQ=255)
                                                                     tGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTAc                                                                            <  1:114937/149‑1 (MQ=255)
                                                                           aGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAATAAGACCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGACTTTGGCGCTTGCAAAACTTACGACCTg                                                                      <  2:291120/149‑1 (MQ=255)
                                                                                gTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGt                                                                 >  2:187410/1‑149 (MQ=255)
                                                                                  gcgcCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTa                                                                <  4:69412/148‑1 (MQ=255)
                                                                                      ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGAt                                                           >  1:289634/1‑149 (MQ=255)
                                                                                         gaagaCTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCAGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATccc                                                        <  1:297564/149‑1 (MQ=255)
                                                                                                    ggcggcGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAac                                              <  3:284767/148‑1 (MQ=255)
                                                                                                                     tGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATctct                            >  2:189400/1‑149 (MQ=255)
                                                                                                                     tGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATctct                            <  1:44588/149‑1 (MQ=255)
                                                                                                                       aCTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTc                          <  2:205001/149‑1 (MQ=255)
                                                                                                                       aCTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTc                          <  1:187410/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACg               >  2:371056/1‑149 (MQ=255)
                                                                                                                                   gAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCgg                                                      <  3:285718/109‑1 (MQ=255)
                                                                                                                                   gAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCgg                                                      >  4:285718/1‑109 (MQ=255)
                                                                                                                                    aaaaaGTCCTGCAGTCGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTcc                         <  3:253780/137‑1 (MQ=255)
                                                                                                                                    aaaaaGTCCTGCAGTCGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTcc                         <  3:148627/137‑1 (MQ=255)
                                                                                                                                    aaaaaGTCCTGCAGTCGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTcc                         >  4:148627/1‑137 (MQ=255)
                                                                                                                                    aaaaaGTCCTGCAGTCGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTcc                         >  4:253780/1‑137 (MQ=255)
                                                                                                                                            cTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAacac                                            <  1:317637/110‑1 (MQ=255)
                                                                                                                                            cTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAacac                                            >  2:317637/1‑110 (MQ=255)
                                                                                                                                              gCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACGTTTGGGATTTcc   >  1:99738/1‑149 (MQ=255)
                                                                                                                                               cAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACGTTTg           >  2:212892/1‑140 (MQ=255)
                                                                                                                                               cAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACGTTTg           <  1:212892/140‑1 (MQ=255)
                                                                                                                                               cAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACGTTTGGGATTTCCa  <  1:189400/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAACTTACGACCTGGAAGTATGGATCCCGGCACAGAACACCTACCGTGAGATCTCTTCCTGCTCCAACGTTTGGGATTTCCA  >  NC_000913/940233‑940524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A