breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 9,907 A→C 11.8% intergenic (+14/+21) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 9,908 G→T 9.4% intergenic (+15/+20) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 10,195 A→C 9.8% G100G (GGT→GGG satP ← succinate‑acetate transporter
RA 22,773 A→T 13.9% E128V (GAA→GTA)  ileS → isoleucyl‑tRNA synthetase
RA 22,776 A→T 14.3% Y129F (TAC→TTC)  ileS → isoleucyl‑tRNA synthetase
RA 191,720 G→A 11.7% intergenic (+12/‑135) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,728 T→C 10.7% intergenic (+20/‑127) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,729 G→T 10.7% intergenic (+21/‑126) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,730 A→C 10.3% intergenic (+22/‑125) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,731 G→A 11.1% intergenic (+23/‑124) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,739 T→C 10.9% intergenic (+31/‑116) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 207,483 A→C 12.8% V786V (GTA→GTC dnaE → DNA polymerase III alpha subunit
RA 207,486 G→T 12.5% M787I (ATG→ATT dnaE → DNA polymerase III alpha subunit
RA 208,552 T→A 7.0% L1143I (TTA→ATA)  dnaE → DNA polymerase III alpha subunit
RA 252,589 G→C 9.8% A97P (GCA→CCA)  yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
RA 256,449 T→C 11.7% intergenic (+14/‑78) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,454 C→G 11.3% intergenic (+19/‑73) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,459 G→A 12.1% intergenic (+24/‑68) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 272,330:1 +G 8.2% intergenic (+141/‑250) insN → / → eyeA pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/novel sRNA, function unknown, CP4‑6 propahge
RA 374,682 C→T 12.1% P272L (CCG→CTG) ‡ mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
RA 374,683 G→C 9.7% P272P (CCG→CCC) ‡ mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
RA 374,688 G→C 9.7% R274P (CGA→CCA) ‡ mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
RA 374,689 A→G 9.5% R274R (CGA→CGG) ‡ mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
RA 381,301 A→T 8.7% intergenic (‑41/‑50) yaiX ← / → insC1 pseudogene, interrupted by IS2A; hexapeptide transferase superfamily; putative transferase/IS2 repressor TnpA
RA 381,302 A→T 8.7% intergenic (‑42/‑49) yaiX ← / → insC1 pseudogene, interrupted by IS2A; hexapeptide transferase superfamily; putative transferase/IS2 repressor TnpA
RA 464,354 C→A 11.8% intergenic (+46/‑48) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,356 A→T 11.5% intergenic (+48/‑46) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,358 T→G 11.3% intergenic (+50/‑44) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 481,225 T→G 13.5% intergenic (‑517/+29) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 481,228 C→A 13.2% intergenic (‑520/+26) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 543,207 A→T 10.2% intergenic (+174/+54) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase
RA 543,210 A→T 10.2% intergenic (+177/+51) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase
RA 569,160 G→T 21.4% D87Y (GAC→TAC) ‡ ybcK → DLP12 prophage; putative recombinase
RA 569,161 A→C 22.0% D87A (GAC→GCC) ‡ ybcK → DLP12 prophage; putative recombinase
RA 639,654 T→C 13.6% intergenic (+146/‑99) ahpC → / → ahpF alkyl hydroperoxide reductase, C22 subunit/alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
RA 639,657 G→A 14.9% intergenic (+149/‑96) ahpC → / → ahpF alkyl hydroperoxide reductase, C22 subunit/alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
RA 644,030 A→G 8.8% intergenic (‑63/+167) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,034 T→C 6.9% intergenic (‑67/+163) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,036 A→T 6.8% intergenic (‑69/+161) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,038 G→A 7.0% intergenic (‑71/+159) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,042 C→T 7.0% intergenic (‑75/+155) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 657,531 T→G 8.7% intergenic (+30/+24) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 657,533 A→T 8.5% intergenic (+32/+22) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 657,535 C→A 8.2% intergenic (+34/+20) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 665,094 G→C 9.3% P33A (CCT→GCT)  rlpA ← septal ring protein, suppressor of prc, minor lipoprotein
RA 710,170 C→T 18.2% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 710,173 A→G 17.4% intergenic (+57/+27) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 770,679 G→T 18.9% intergenic (+68/‑779) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
RA 770,682 A→C 19.4% intergenic (+71/‑776) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
RA 782,999 T→G 8.9% L305L (CTT→CTG nadA → quinolinate synthase, subunit A
RA 783,000 C→A 8.7% Q306K (CAG→AAG)  nadA → quinolinate synthase, subunit A
RA 805,312 G→T 9.3% E604* (GAG→TAG) ‡ ybhJ → aconitase family protein
RA 805,313 A→C 9.3% E604A (GAG→GCG) ‡ ybhJ → aconitase family protein
RA 847,203 T→C 9.8% intergenic (‑84/+55) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit
RA 857,576 T→C 13.5% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,578 C→G 13.5% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,580 G→A 13.5% intergenic (+25/+216) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 940,380 T→C 8.3% L318S (TTG→TCG)  serS → seryl‑tRNA synthetase
RA 940,751 T→C 15.9% intergenic (+31/‑208) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 940,754 G→A 16.7% intergenic (+34/‑205) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 1,015,912 T→A 10.7% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
RA 1,153,208 T→C 12.0% intergenic (+28/‑92) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
RA 1,153,213 T→A 11.8% intergenic (+33/‑87) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
RA 1,153,214 T→A 11.8% intergenic (+34/‑86) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
RA 1,153,219 G→A 12.5% intergenic (+39/‑81) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
RA 1,224,239 A→T 9.1% intergenic (+332/+40) ycgI → / ← minE pseudogene/cell division topological specificity factor
RA 1,224,242 A→T 7.5% intergenic (+335/+37) ycgI → / ← minE pseudogene/cell division topological specificity factor
RA 1,250,962 T→C 12.4% intergenic (‑124/‑104) dhaK ← / → dhaR dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator
RA 1,250,963 A→T 12.3% intergenic (‑125/‑103) dhaK ← / → dhaR dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator
RA 1,250,964 G→A 12.2% intergenic (‑126/‑102) dhaK ← / → dhaR dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator
RA 1,285,190 T→A 16.3% L18I (TTA→ATA)  narJ → molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
RA 1,285,193 T→A 16.3% W19R (TGG→AGG)  narJ → molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
RA 1,292,472 G→A 10.2% intergenic (+107/+37) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
RA 1,292,473 C→G 10.3% intergenic (+108/+36) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
RA 1,292,474 T→C 10.0% intergenic (+109/+35) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
RA 1,330,396:1 +T 7.5% intergenic (+15/+21) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
RA 1,330,399 A→T 7.4% intergenic (+18/+18) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
RA 1,330,402 Δ1 bp 7.4% intergenic (+21/+15) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
RA 1,375,088 G→C 9.4% V42V (GTG→GTC ycjQ → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,375,090 T→A 9.2% V43D (GTC→GAC)  ycjQ → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,375,092 G→C 9.6% D44H (GAC→CAC)  ycjQ → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,446,357:1 +T 12.5% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB
RA 1,446,362 Δ1 bp 13.3% intergenic (+156/+16) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB
RA 1,449,050 T→A 13.2% intergenic (+32/+26) feaB → / ← tynA phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring
RA 1,493,194 G→C 9.3% S242T (AGT→ACT)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,608,704 A→G 13.3% S107P (TCG→CCG)  yneE ← bestrophin family putative inner membrane protein
RA 1,608,707 C→T 13.3% D106N (GAT→AAT)  yneE ← bestrophin family putative inner membrane protein
RA 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,698,585 T→G 12.2% E199A (GAA→GCA)  malI ← transcriptional repressor of Mal regulon
RA 1,698,587 T→A 11.6% A198A (GCA→GCT) ‡ malI ← transcriptional repressor of Mal regulon
RA 1,698,589 C→A 12.2% A198S (GCA→TCA) ‡ malI ← transcriptional repressor of Mal regulon
RA 1,727,292 G→A 8.7% A212T (GCA→ACA)  nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
RA 1,757,688 T→A 16.6% intergenic (+31/+33) lpp → / ← ldtE murein lipoprotein/murein L,D‑transpeptidase
RA 1,795,227 A→T 22.2% intergenic (‑75/+26) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,795,228 G→C 22.2% intergenic (‑76/+25) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,795,229 A→T 22.2% intergenic (‑77/+24) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,799,169 T→G 10.3% intergenic (‑227/+57) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
RA 1,799,170 G→T 10.5% intergenic (‑228/+56) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
RA 1,880,851 T→A 14.3% intergenic (‑92/+35) leuE ← / ← dmlR leucine efflux protein/transcriptional activator of dmlA
RA 1,880,852 T→A 14.3% intergenic (‑93/+34) leuE ← / ← dmlR leucine efflux protein/transcriptional activator of dmlA
RA 1,955,176 Δ1 bp 14.7% coding (1832/2430 nt) torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,985,112 C→G 19.7% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
RA 2,062,354 T→A 8.7% intergenic (+19/+37) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,355 T→G 8.7% intergenic (+20/+36) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,356 C→A 8.7% intergenic (+21/+35) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,357 T→A 8.7% intergenic (+22/+34) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,081,688 A→T 16.7% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
RA 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,206,298 C→T 16.0% H202Y (CAC→TAC)  yehM → uncharacterized protein
RA 2,261,044 C→T 9.0% A123T (GCT→ACT)  fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
RA 2,270,602 T→C 11.9% intergenic (+57/‑124) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,270,605 G→A 11.6% intergenic (+60/‑121) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,282,830 T→C 16.7% intergenic (+29/+110) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,282,831 C→G 17.2% intergenic (+30/+109) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,282,832 C→G 16.8% intergenic (+31/+108) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,282,833 G→A 17.2% intergenic (+32/+107) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,318,095 C→G 100% L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,325,037:1 +T 11.4% coding (282/1323 nt) atoE → short chain fatty acid transporter
RA 2,325,042 Δ1 bp 11.4% coding (287/1323 nt) atoE → short chain fatty acid transporter
RA 2,333,310 T→C 8.0% pseudogene (1093/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,414,689 C→G 17.1% intergenic (+17/‑58) ackA → / → pta acetate kinase A and propionate kinase 2/phosphate acetyltransferase
RA 2,440,865 C→T 8.7% L247L (CTG→CTA) ‡ fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,440,866 A→G 8.7% L247P (CTG→CCG) ‡ fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,527,849 A→G 9.1% I302V (ATC→GTC)  yfeH → putative inorganic ion transporter
RA 2,536,370 C→G 18.2% intergenic (+27/+16) crr → / ← pdxK glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
RA 2,536,373 C→G 16.0% intergenic (+30/+13) crr → / ← pdxK glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
RA 2,550,574 C→G 16.4% intergenic (‑29/+67) yfeX ← / ← yfeY porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein
RA 2,550,576 A→T 16.7% intergenic (‑31/+65) yfeX ← / ← yfeY porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein
RA 2,550,578 C→G 17.6% intergenic (‑33/+63) yfeX ← / ← yfeY porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein
RA 2,566,266 C→A 8.8% G207C (GGC→TGC)  eutA ← reactivating factor for ethanolamine ammonia lyase
RA 2,566,267 T→G 8.7% A206A (GCA→GCC eutA ← reactivating factor for ethanolamine ammonia lyase
RA 2,589,122 G→A 8.2% G510S (GGC→AGC)  acrD → aminoglycoside/multidrug efflux system
RA 2,589,125 T→C 8.3% F511L (TTT→CTT)  acrD → aminoglycoside/multidrug efflux system
RA 2,630,079 G→A 11.1% T96T (ACG→ACA yfgH → outer membrane integrity lipoprotein
RA 2,707,320 A→T 9.4% intergenic (‑196/+2) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
RA 2,707,321 A→T 9.6% intergenic (‑197/+1) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
RA 2,716,030 T→G 8.5% intergenic (+20/+36) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
RA 2,716,033 C→A 9.0% intergenic (+23/+33) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
RA 2,724,095 C→A 7.4% intergenic (+13/‑33) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
RA 2,724,097 T→A 7.4% intergenic (+15/‑31) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
RA 2,724,099 T→G 7.0% intergenic (+17/‑29) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
RA 2,761,620 T→G 7.0% K641Q (AAG→CAG)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,761,623 C→A 7.0% V640L (GTA→TTA)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,778,123 G→T 16.0% intergenic (+341/+23) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,778,124 A→C 16.8% intergenic (+342/+22) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,800,515 C→A 11.4% intergenic (+40/‑208) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
RA 2,800,517 T→A 11.1% intergenic (+42/‑206) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
RA 2,800,519 T→G 10.8% intergenic (+44/‑204) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
RA 2,838,222 A→T 11.1% intergenic (‑117/+32) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
RA 2,850,154 C→A 6.6% Q94H (CAG→CAT hycA ← regulator of the transcriptional regulator FhlA
RA 2,911,007 G→A 7.8% R29C (CGT→TGT)  mazF ← mRNA interferase toxin, antitoxin is MazE
RA 2,911,008 A→T 7.7% H28Q (CAT→CAA) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE
RA 2,911,009 T→C 7.5% H28R (CAT→CGT) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE
RA 3,066,319 Δ1 bp 10.0% coding (855/897 nt) ygfI ← putative DNA‑binding transcriptional regulator
RA 3,156,590 C→A 13.6% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,156,591 T→G 12.9% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,183,345:1 +T 10.3% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,183,350 Δ1 bp 10.0% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,239,609 T→G 9.8% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,239,610 C→G 9.6% intergenic (+65/‑334) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,239,611 C→A 9.6% intergenic (+66/‑333) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,250,727 T→C 8.9% F56L (TTT→CTT)  yqjF → putative quinol oxidase subunit
RA 3,313,207 G→A 18.9% intergenic (‑29/+135) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,313,208 T→A 18.9% intergenic (‑30/+134) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,313,209 T→C 18.9% intergenic (‑31/+133) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,318,079 A→T 7.8% intergenic (‑73/+134) rimP ← / ← metY ribosome maturation factor for 30S subunits/tRNA‑Met
RA 3,318,080 A→T 7.8% intergenic (‑74/+133) rimP ← / ← metY ribosome maturation factor for 30S subunits/tRNA‑Met
RA 3,332,822 G→A 25.0% intergenic (‑87/+40) yhbE ← / ← rpmA EamA family inner membrane putative transporter/50S ribosomal subunit protein L27
RA 3,332,823 T→C 24.7% intergenic (‑88/+39) yhbE ← / ← rpmA EamA family inner membrane putative transporter/50S ribosomal subunit protein L27
RA 3,470,106 C→T 8.7% intergenic (‑253/+39) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1
RA 3,470,107 G→T 8.4% intergenic (‑254/+38) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1
RA 3,470,108 A→C 8.7% intergenic (‑255/+37) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1
RA 3,470,109 A→G 8.7% intergenic (‑256/+36) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1
RA 3,545,698 C→A 8.9% I25I (ATC→ATA nfuA → Fe/S biogenesis protein, putative scaffold/chaperone protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,569,431 A→G 9.8% I630T (ATT→ACT)  glgX ← glycogen debranching enzyme
RA 3,577,657 A→G 8.5% intergenic (‑65/+74) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,577,660 C→T 7.0% intergenic (‑68/+71) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,629,495 A→T 12.8% L15Q (CTG→CAG)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,629,498 T→C 8.9% Q14R (CAA→CGA)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,629,503 G→A 13.8% V12V (GTC→GTT rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,629,506 A→T 14.0% P11P (CCT→CCA rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,642,358 T→A 11.4% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,642,361 T→A 10.8% intergenic (+30/+19) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,722,300 G→T 5.4% intergenic (+195/+28) sokA → / ← glyS pseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit
RA 3,728,215 T→A 7.0% K386I (AAA→ATA)  xylB ← xylulokinase
RA 3,728,217 A→T 6.9% I385I (ATT→ATA) ‡ xylB ← xylulokinase
RA 3,728,219 T→A 6.9% I385F (ATT→TTT) ‡ xylB ← xylulokinase
RA 3,754,924 G→T 7.1% intergenic (‑496/+49) yiaW ← / ← aldB DUF3302 family inner membrane protein/aldehyde dehydrogenase B
RA 3,754,927 A→C 6.7% intergenic (‑499/+46) yiaW ← / ← aldB DUF3302 family inner membrane protein/aldehyde dehydrogenase B
RA 3,781,051 C→G 6.9% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,790,300 A→T 9.7% intergenic (‑219/+20) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,790,303 A→T 9.3% intergenic (‑222/+17) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,810,321 T→C 5.9% intergenic (+17/+22) coaD → / ← mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
RA 3,810,324 G→A 5.9% intergenic (+20/+19) coaD → / ← mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
RA 3,814,164 G→A 5.0% G78D (GGT→GAT)  dut → deoxyuridinetriphosphatase
RA 3,815,816 C→G 5.2% intergenic (‑48/+18) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,815,819 C→G 5.1% intergenic (‑51/+15) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,828,334 A→T 5.5% I111N (ATT→AAT)  gltS ← glutamate transporter
RA 3,832,797 T→G 6.2% N581H (AAC→CAC)  yicI ← putative alpha‑glucosidase
RA 3,832,800 C→A 6.3% A580S (GCG→TCG)  yicI ← putative alpha‑glucosidase
RA 3,991,334 T→A 8.6% I61N (ATT→AAT) ‡ cyaA → adenylate cyclase
RA 3,991,335 T→A 8.6% I61I (ATT→ATA) ‡ cyaA → adenylate cyclase
RA 3,993,145 C→A 100% R665S (CGT→AGT)  cyaA → adenylate cyclase
JC 3,998,555 Δ3 bp 9.5% coding (573‑575/2163 nt) uvrD → DNA‑dependent ATPase I and helicase II
RA 4,015,333 A→T 14.3% intergenic (+19/+21) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
RA 4,070,410 C→A 12.2% intergenic (‑9/+105) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,070,413 T→G 14.5% intergenic (‑12/+102) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,117,047 G→T 100% S59Y (TCC→TAC)  glpK ← glycerol kinase
JC 4,130,635 Δ13 bp 27.0% coding (801‑813/2433 nt) metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,142,509 T→G 16.7% intergenic (‑288/‑21) frwA ← / → frwC putative PTS enzyme: Hpr, enzyme I and II components/putative enzyme IIC component of PTS
RA 4,142,512 C→A 16.7% intergenic (‑291/‑18) frwA ← / → frwC putative PTS enzyme: Hpr, enzyme I and II components/putative enzyme IIC component of PTS
RA 4,148,049 C→G 12.8% G90A (GGC→GCC)  yijO ← AraC family putative transcriptional activator
RA 4,177,171 T→A 22.8% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
RA 4,177,172 T→A 21.6% intergenic (+44/‑186) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
RA 4,183,165 G→A 100% E641K (GAA→AAA)  rpoB → RNA polymerase, beta subunit
RA 4,227,550 G→A 8.6% intergenic (+39/‑181) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
RA 4,227,555 A→T 7.9% intergenic (+44/‑176) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
RA 4,227,560 T→C 7.4% intergenic (+49/‑171) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
RA 4,262,250 G→C 9.6% G194G (GGG→GGC dusA → tRNA‑dihydrouridine synthase A
RA 4,262,255 G→C 9.6% S196T (AGC→ACC)  dusA → tRNA‑dihydrouridine synthase A
RA 4,291,512 G→A 11.3% M1M (GTG→ATG) †‡ nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
RA 4,291,513 T→C 11.3% M1A (GTG→GCG) †‡ nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
RA 4,296,060 C→T 20.3% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,362,528 G→A 12.2% intergenic (‑175/+23) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,362,529 T→C 12.2% intergenic (‑176/+22) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,409,402 Δ1 bp 11.7% coding (128/732 nt) rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,409,407 T→A 12.5% S45T (TCT→ACT)  rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,409,410:1 +A 13.0% coding (136/732 nt) rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,422,887 G→A 9.3% A14A (GCG→GCA ulaE → L‑xylulose 5‑phosphate 3‑epimerase
RA 4,424,465 G→T 8.3% intergenic (+79/+51) ulaF → / ← yjfY L‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic
RA 4,424,468 A→C 8.3% intergenic (+82/+48) ulaF → / ← yjfY L‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic
RA 4,447,964 T→A 10.3% L24* (TTA→TAA)  ytfP → GGCT‑like protein
RA 4,472,434 G→A 17.0% R34* (CGA→TGA)  pyrL ← pyrBI operon leader peptide
RA 4,472,437 T→C 16.7% N33D (AAT→GAT)  pyrL ← pyrBI operon leader peptide
RA 4,496,511 A→G 8.5% intergenic (+22/‑164) leuX → / → intB tRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase
RA 4,496,512 C→T 8.3% intergenic (+23/‑163) leuX → / → intB tRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase
RA 4,520,500 Δ1 bp 7.4% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 7.1% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,604,753 T→A 14.0% S198R (AGT→AGA bglJ → bgl operon transcriptional activator
RA 4,604,754 T→A 15.4% S199T (TCT→ACT)  bglJ → bgl operon transcriptional activator
RA 4,608,604 C→G 9.3% I140M (ATC→ATG rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase
RA 4,608,607 C→G 9.3% I141M (ATC→ATG rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423783–3424234 3424545–3424239 6–763 14 [13] [13] 17 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 233792 =34 (0.780)4 (0.090) 4/250 NT 11.2% coding (164/1359 nt) mltD putative membrane‑bound lytic murein transglycosylase D
?NC_000913 233828 = 30 (0.700)coding (128/1359 nt) mltD putative membrane‑bound lytic murein transglycosylase D
* ? NC_000913 412077 =38 (0.870)5 (0.120) 4/240 NT 12.7% coding (405/1185 nt) araJ L‑arabinose‑inducible putative transporter, MFS family
?NC_000913 412119 = 33 (0.800)coding (363/1185 nt) araJ L‑arabinose‑inducible putative transporter, MFS family
* ? NC_000913 1207790 =13 (0.300)18 (0.470) 18/224 NT 59.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 13 (0.340)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780512 (0.270)12 (0.310) 12/224 NT 50.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 13 (0.340)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)40 (0.940) 37/248 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 146344547 (1.070)5 (0.120) 4/242 NT 10.3% intergenic (+7/‑94) paaK/paaX phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
?NC_000913 = 1463482 43 (1.040)intergenic (+44/‑57) paaK/paaX phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
* ? NC_000913 3588334 =27 (0.620)3 (0.070) 3/242 NT 10.3% coding (847/1071 nt) ugpC sn‑glycerol‑3‑phosphate ABC transporter ATPase
?NC_000913 3588367 = 27 (0.650)coding (814/1071 nt) ugpC sn‑glycerol‑3‑phosphate ABC transporter ATPase
* ? NC_000913 3721045 =66 (1.510)135 (3.100) 95/254 NT 71.5% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 42 (0.970)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28
* ? NC_000913 = 429616710 (0.230)6 (0.140) 5/242 NT 38.8% noncoding (333/600 nt) RIP321 (repetitive extragenic palindromic) element; contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element; contains 11 REP sequences and 4 IHF sites
?NC_000913 = 4296250 NA (NA)noncoding (416/600 nt) RIP321 (repetitive extragenic palindromic) element; contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element; contains 11 REP sequences and 4 IHF sites
* ? NC_000913 4377614 =31 (0.710)4 (0.100) 4/244 NT 12.0% coding (109/534 nt) blc outer membrane lipoprotein cell division and growth lipocalin
?NC_000913 4377646 = 29 (0.690)coding (77/534 nt) blc outer membrane lipoprotein cell division and growth lipocalin