Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F216 I0 R1 247 44.8 1629152 98.0% 1596568 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,799,169 T→G 10.3% intergenic (‑227/+57) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,1690TG10.3% 117.8 / 7.3 39intergenic (‑227/+57)pheS/pheMphenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
Reads supporting (aligned to +/- strand):  ref base T (16/19);  new base G (2/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

TTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAA  >  NC_000913/1799022‑1799315
                                                                                                                                                   |                                                                                                                                                  
ttGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTg                                                                                                                                                   <  1:127560/149‑1 (MQ=255)
       cGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGc                                                                                                                                             >  3:223233/1‑148 (MQ=255)
          gCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCCAGTGGAGGCttt                                                                                                                                          <  4:181251/148‑1 (MQ=255)
           cTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCCAGTGGAGGCttttt                                                                                                                                        <  1:379551/149‑3 (MQ=255)
            tgtgCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAg                                                                                                                                       <  1:74046/149‑1 (MQ=255)
                  cGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCt                                                                                                                                  >  3:103833/1‑148 (MQ=255)
                   gAAATTCTGACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGt                                                                                                                                <  2:351385/149‑1 (MQ=255)
                   gAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGt                                                                                                                                >  2:279962/1‑149 (MQ=255)
                     aaTTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCACGCGCTGttt                                                                                                                              <  2:308215/149‑1 (MQ=255)
                       ttCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTc                                                                                                                            <  2:379547/149‑1 (MQ=255)
                               tACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTc                                                                                                                                         >  4:183112/1‑128 (MQ=255)
                               tACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTc                                                                                                                                         <  3:183112/128‑1 (MQ=255)
                                   tAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTttctcttc                                                                                                                 <  3:125642/148‑1 (MQ=255)
                                            tCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCt                                                                                                        >  3:228462/1‑148 (MQ=255)
                                             ccGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTa                                                                                                       >  4:194462/1‑148 (MQ=255)
                                              cGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAg                                                                                                      <  3:194462/148‑1 (MQ=255)
                                                   tctcCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAAGCTTTTTATTTGTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGcctcc                                                                                                 <  4:111707/148‑1 (MQ=255)
                                                          gcTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGAGAGGCTTTTTATTTTTCAAAACGCGCATAC‑AAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCa                                                                                         <  4:266424/148‑1 (MQ=255)
                                                                  tgtCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTa                                                                                  >  4:39276/1‑148 (MQ=255)
                                                                     ccAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTACACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATCCAGGTGCTAAAg                                                                               >  3:137715/1‑148 (MQ=255)
                                                                             gCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAaag                                                                      >  1:370806/1‑149 (MQ=255)
                                                                                 cGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAaagaag                                                                   <  4:132880/148‑1 (MQ=255)
                                                                                           acCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATagc                                                        >  1:304987/1‑149 (MQ=255)
                                                                                            ccTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATagc                                                        <  4:158667/148‑1 (MQ=255)
                                                                                                             tttATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCg                                       >  3:205492/1‑148 (MQ=255)
                                                                                                             tttAATTTTCACAATGCGCATACAAAAAAATCATCCACTGGGAGGATTTCAGGCGGTGGTTTCCGTTTATCTTCTCAAGCGCTAGCGCCATGGATTCAGGCGCTAAAGTAAAAAAAGAAGCGGAAACTGACAGCATTCAttgattgcat                                      >  2:343363/1‑147 (MQ=255)
                                                                                                                      cAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt                              <  4:228462/148‑1 (MQ=255)
                                                                                                                          aCGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTa                          >  4:26940/1‑148 (MQ=255)
                                                                                                                              gcATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCtt                     <  2:370806/149‑1 (MQ=255)
                                                                                                                               cATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCtt                     >  3:371671/1‑148 (MQ=255)
                                                                                                                                   cAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCaaaa                <  1:130906/149‑1 (MQ=255)
                                                                                                                                   cAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCaaaa                <  1:279962/149‑1 (MQ=255)
                                                                                                                                     ctgaaaGCCTCCCAGTGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGa              >  2:274697/4‑149 (MQ=255)
                                                                                                                                       gaaaGCCTCCGAGTGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGAc             >  3:236967/2‑148 (MQ=255)
                                                                                                                                            ccTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTAt       <  2:248517/149‑1 (MQ=255)
                                                                                                                                               ccACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTg     >  3:323185/1‑148 (MQ=255)
                                                                                                                                                cACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGaa   >  2:53845/1‑149 (MQ=255)
                                                                                                                                                cACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGa    <  3:386985/148‑1 (MQ=255)
                                                                                                                                                  cTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGaaa  <  4:107079/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAA  >  NC_000913/1799022‑1799315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A