Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F216 I0 R1
|
247 |
44.8 |
1629152 |
98.0% |
1596568 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,282,832 |
C→G |
16.8% |
intergenic (+31/+108) |
rplY → / ← yejK |
50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,282,832 | 0 | C | G | 16.8%
| 83.6
/ 6.4
| 29 | intergenic (+31/+108) | rplY/yejK | 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein |
| Reads supporting (aligned to +/- strand): ref base C (11/13); new base G (3/2); total (14/15) |
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGG > NC_000913/2282693‑2282973
|
aGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtt < 2:71610/149‑1 (MQ=255)
tCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGttttt < 1:333606/149‑1 (MQ=255)
aaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGAt < 2:166585/149‑1 (MQ=255)
aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGttttttacaa < 3:392250/130‑5 (MQ=39)
aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGttttttacaa > 4:392250/1‑126 (MQ=39)
gAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTa > 2:14301/1‑149 (MQ=255)
aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTg > 4:259799/1‑138 (MQ=255)
aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTg < 3:259799/138‑1 (MQ=255)
aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt < 1:401116/149‑1 (MQ=255)
aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt > 4:162288/1‑148 (MQ=255)
ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGAt > 2:144843/1‑137 (MQ=255)
ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGAt < 1:144843/137‑1 (MQ=255)
cAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGCATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTg > 1:154389/1‑149 (MQ=255)
gACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg < 1:257271/149‑1 (MQ=255)
gTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa < 4:321705/148‑1 (MQ=255)
cGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGttttt < 4:391885/87‑1 (MQ=255)
cGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGttttt > 3:391885/1‑87 (MQ=255)
aaGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGTGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATc < 3:348627/148‑1 (MQ=255)
caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa < 2:134670/149‑1 (MQ=255)
ttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTg < 2:289754/128‑1 (MQ=255)
ttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTg > 1:289754/1‑128 (MQ=255)
caaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGc < 2:364438/85‑1 (MQ=37)
caaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGc > 1:364438/2‑86 (MQ=37)
aaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc > 4:81076/1‑148 (MQ=255)
aaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa > 3:156475/1‑120 (MQ=38)
ccccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAAtt > 3:341791/1‑148 (MQ=255)
cccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt > 2:236678/1‑62 (MQ=255)
cccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt < 1:236678/62‑1 (MQ=255)
ccGCTCCGGCGGGGTTTTTTGTATCTGAAGATTATGCCTGATTACAGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTgg > 4:248802/1‑148 (MQ=255)
|
AGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGG > NC_000913/2282693‑2282973
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
