Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F5 I0 R1
|
408 |
67.6 |
2494072 |
96.8% |
2414261 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,836,136 |
G→C |
11.1% |
D22H (GAC→CAC) |
ynjB → |
putative ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,836,136 | 0 | G | C | 11.1%
| 180.0
/ 13.5
| 54 | D22H (GAC→CAC) | ynjB | putative ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (23/25); new base C (2/4); total (25/29) |
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 |
TCAGCCCGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTC > NC_000913/1835992‑1836280
|
tCAGCCCGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTg > 4:62599/1‑149 (MQ=255)
cccGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCaa < 3:79649/149‑1 (MQ=255)
cccGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCaa > 3:449021/1‑149 (MQ=255)
cccGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCaa < 2:501525/149‑1 (MQ=255)
cccGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTCACTGGCat < 2:469476/149‑2 (MQ=255)
cccGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTCACTGGCat > 1:551588/1‑148 (MQ=255)
cGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTCACTGGCatgt < 3:226707/149‑4 (MQ=255)
gTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCATATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGaa > 1:276398/1‑149 (MQ=255)
ttttAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGaaa > 2:452177/1‑149 (MQ=255)
ttttAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGaaa > 1:373886/1‑149 (MQ=255)
tttAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTCACTGGCatgtgtt < 2:551588/149‑7 (MQ=255)
ttCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACTTGCCAGTGACTGGCAAGAAATTaaaa < 4:262059/149‑1 (MQ=255)
cTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAAt < 3:573950/149‑1 (MQ=255)
cTGCCATGGACATTCCCGCTCAGGGGCTGTGCCATATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAAt < 2:276398/149‑1 (MQ=255)
tGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGAc > 2:28444/1‑130 (MQ=255)
tGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGAc < 1:28444/130‑1 (MQ=255)
ccATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGTCAGTGACTGGCAAGAAATTAAAAATGAg < 4:169484/149‑1 (MQ=255)
ccGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCaa < 1:452177/149‑1 (MQ=255)
ggggCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCt < 4:292477/149‑1 (MQ=255)
tgtgCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGttt > 4:56991/1‑149 (MQ=255)
gCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAAc < 2:401896/149‑1 (MQ=255)
cTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGggc > 3:496277/1‑149 (MQ=255)
aaaCGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGa < 4:449021/149‑1 (MQ=255)
aaaCGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGa > 3:277231/1‑149 (MQ=255)
cGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATAc < 3:18313/149‑1 (MQ=255)
gTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATAcc < 2:373886/149‑1 (MQ=255)
ttAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCaa > 1:399483/1‑149 (MQ=255)
ttAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCaa > 1:588823/1‑149 (MQ=255)
tgCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTAtctc > 4:450198/1‑149 (MQ=255)
aTTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTgg < 2:141694/149‑1 (MQ=255)
gtggtTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAAcc < 1:258162/128‑1 (MQ=255)
gtggtTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAAcc > 2:258162/1‑128 (MQ=255)
tGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATg > 4:355150/1‑149 (MQ=255)
aTTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGaaaa > 1:329141/1‑149 (MQ=255)
aTTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGaaaa < 3:56991/149‑1 (MQ=255)
ttATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTgg > 1:116079/1‑126 (MQ=255)
ttATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTgg < 2:116079/126‑1 (MQ=255)
ttATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTgg < 2:125088/126‑1 (MQ=255)
ttATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTgg > 1:125088/1‑126 (MQ=255)
ttATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCa > 1:175706/1‑149 (MQ=255)
gCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACg > 1:107837/1‑149 (MQ=255)
cTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGc < 2:588823/149‑1 (MQ=255)
cTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGc > 1:188375/1‑149 (MQ=255)
tGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGaaaa < 3:541763/138‑1 (MQ=255)
tGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGaaaa > 4:541763/1‑138 (MQ=255)
gTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCta < 3:62599/149‑1 (MQ=255)
tctcTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATaaa < 4:496277/149‑1 (MQ=255)
ggCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGa < 4:277231/149‑1 (MQ=255)
gCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGaa < 2:329141/149‑1 (MQ=255)
gCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGaa > 2:400445/1‑149 (MQ=255)
ttcttGCCAGTCACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAAc > 4:160947/5‑124 (MQ=255)
ttcttGCCAGTCACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAAc < 3:160947/120‑1 (MQ=255)
tGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTcc < 3:124289/149‑1 (MQ=255)
cAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTc > 3:179223/1‑149 (MQ=255)
|
TCAGCCCGTTTTAATTCTGCCATGGACATTCCCGCTCAGGGGCTGTGCCGTATTCCTGAAAAACGTTCTTAAGGAGAGATGATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTC > NC_000913/1835992‑1836280
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
