Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F5 I0 R1
|
408 |
67.6 |
2494072 |
96.8% |
2414261 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,987,859 |
G→C |
10.2% |
intergenic (‑101/+14) |
yecJ ← / ← azuC |
DUF2766 family protein/acid‑inducible small membrane‑associated protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,987,859 | 0 | G | C | 10.2%
| 162.2
/ 9.8
| 49 | intergenic (‑101/+14) | yecJ/azuC | DUF2766 family protein/acid‑inducible small membrane‑associated protein |
| Reads supporting (aligned to +/- strand): ref base G (22/22); new base C (2/3); total (24/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 |
CATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTG > NC_000913/1987716‑1988002
|
caTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAg < 4:214452/149‑1 (MQ=255)
cAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGtt < 2:337660/149‑1 (MQ=255)
aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑TCCGGCAGGtttt > 4:237749/1‑148 (MQ=255)
aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑TCCGGCAGGtttt < 3:237749/148‑1 (MQ=255)
aaCCAATTCCTGATCGGCATTCAGCGGCTAGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAATAGAACCTGC‑CGGAGCAGGtttttt < 2:24654/149‑1 (MQ=255)
cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCATCGTCATCAGGACACAAAAAAACGTGCTCCG‑GCAGGttttttt < 3:364963/147‑2 (MQ=255)
cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑TCCGGCAGGttttttt > 4:364963/1‑146 (MQ=255)
cTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGa > 1:521033/1‑149 (MQ=255)
aTTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGc > 1:51074/1‑149 (MQ=255)
aGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTgg > 2:27719/1‑149 (MQ=255)
gCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGt > 4:456523/1‑149 (MQ=255)
gCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGt < 1:214338/149‑1 (MQ=255)
gACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTc > 2:3391/1‑149 (MQ=255)
tAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc > 2:280930/1‑149 (MQ=255)
cTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATAtt > 1:95998/1‑123 (MQ=255)
cTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATAtt < 2:95998/123‑1 (MQ=255)
tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCTCCG‑GCAGGttttttt > 4:214413/1‑105 (MQ=38)
tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGC‑TCCGGCAGGttttttt < 3:214413/106‑2 (MQ=38)
cTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGc < 1:27719/149‑1 (MQ=255)
tAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa < 1:280930/149‑1 (MQ=255)
aTTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc < 2:268200/134‑1 (MQ=255)
aTTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc > 1:268200/1‑134 (MQ=255)
aTTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTa > 4:23781/1‑149 (MQ=255)
tataGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACtttt < 3:390693/149‑1 (MQ=255)
tagtagCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCa < 1:3391/149‑1 (MQ=255)
tagCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGa > 4:403039/1‑149 (MQ=255)
acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc < 2:489428/105‑1 (MQ=255)
acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc > 1:489428/1‑105 (MQ=255)
aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt > 2:162057/1‑149 (MQ=255)
tCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGttt > 3:568061/1‑149 (MQ=255)
tCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGttt > 4:508053/1‑149 (MQ=255)
tCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCAt < 2:51074/149‑1 (MQ=255)
aTAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGt < 2:203097/138‑1 (MQ=255)
aTAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGAGCAGt > 1:203097/1‑138 (MQ=255)
aTCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc > 3:476542/1‑149 (MQ=255)
ccGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt < 1:162057/149‑1 (MQ=255)
cGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTg < 3:403039/149‑1 (MQ=255)
tcaGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata < 3:456523/149‑1 (MQ=255)
tcaGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata < 4:476542/149‑1 (MQ=255)
acaAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc < 4:396088/70‑1 (MQ=255)
acaAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc > 3:396088/1‑70 (MQ=255)
aaaaaaaCCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt < 2:520956/145‑1 (MQ=255)
aaaaaaaCCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt > 1:520956/1‑145 (MQ=255)
aaaaaaCCTGC‑TCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGa < 2:31974/98‑1 (MQ=39)
aaaaaaCCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt < 2:521033/149‑1 (MQ=255)
aaaaCCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTg > 1:449161/1‑149 (MQ=255)
ccTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCtt > 1:589517/1‑149 (MQ=255)
cTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCttt < 3:508053/149‑1 (MQ=255)
tGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTg > 4:230065/1‑149 (MQ=255)
|
CATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGC‑CGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTG > NC_000913/1987716‑1988002
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
