Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F36 I0 R2
|
1302 |
104.9 |
4551290 |
96.3% |
4382892 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,187,314 |
C→G |
22.2% |
intergenic (+16/+66) |
rcnB → / ← yehA |
periplasmic modulator of Ni and Co efflux/putative fimbrial‑like adhesin protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,187,314 | 0 | C | G | 22.2%
| 36.4
/ 8.5
| 18 | intergenic (+16/+66) | rcnB/yehA | periplasmic modulator of Ni and Co efflux/putative fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base C (7/7); new base G (2/2); total (9/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 |
TGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGT > NC_000913/2187234‑2187453
|
tGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAggg < 1:249115/88‑1 (MQ=255)
tGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAggg > 2:249115/1‑88 (MQ=255)
tCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCt < 4:672930/108‑1 (MQ=255)
tCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCt > 3:672930/1‑108 (MQ=255)
tAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGa > 1:1008342/1‑57 (MQ=255)
tAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGa < 2:1008342/57‑1 (MQ=255)
ccTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttg > 2:648253/1‑106 (MQ=255)
ccTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttg < 1:648253/106‑1 (MQ=255)
tttttATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGAttt > 1:248515/1‑148 (MQ=255)
atcatcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg > 2:362847/1‑96 (MQ=255)
atcatcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg < 1:362847/96‑1 (MQ=255)
cGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgttt < 2:248515/148‑1 (MQ=255)
aGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTaa > 1:654184/1‑101 (MQ=255)
aGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTaa < 2:654184/101‑1 (MQ=255)
cccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGt > 2:323151/1‑78 (MQ=255)
cccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGt < 1:323151/78‑1 (MQ=255)
cTCATCATGAGGGGGAAATGCAGACACCTTGTTAttt > 1:702014/1‑37 (MQ=255)
cTCATCATGAGGGGGAAATGCAGACACCTTGTTAttt < 2:702014/37‑1 (MQ=255)
catGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt > 2:639898/1‑140 (MQ=255)
catGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt < 1:639898/140‑1 (MQ=255)
|
TGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGT > NC_000913/2187234‑2187453
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
