Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F68 I0 R1 683 78.3 2772122 98.0% 2716679 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,799,374:1 +T 5.7% intergenic (‑104/+19) pheM ← / ← rplT phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,3741.T 5.7% 371.4 / 16.2 87intergenic (‑104/+19)pheM/rplTphenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20
Reads supporting (aligned to +/- strand):  ref base . (45/37);  new base T (2/3);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGC  >  NC_000913/1799233‑1799520
                                                                                                                                              |                                                                                                                                                  
cTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctc                                                                                                                                             <  1:252976/149‑1 (MQ=255)
 tAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct                                                                                                                                            >  4:24920/1‑149 (MQ=255)
 tAAAGT‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCtt                                                                                                                                           <  3:38975/149‑1 (MQ=255)
  aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCtt                                                                                                                                           <  3:24920/149‑1 (MQ=255)
  aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCtt                                                                                                                                           >  4:270251/1‑149 (MQ=255)
  aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCCTCtt                                                                                                                                           <  1:65328/149‑1 (MQ=255)
    aGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCCTCtttt                                                                                                                                         <  4:382821/149‑2 (MQ=255)
     gTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCa                                                                                                                                        >  4:142847/1‑149 (MQ=255)
      tAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCaa                                                                                                                                       <  1:17983/149‑1 (MQ=255)
        aaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACt                                                                                                                                     >  2:519482/1‑149 (MQ=255)
           aaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGc                                                                                                                                  >  4:72303/1‑149 (MQ=255)
           aaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGc                                                                                                                                  >  3:483109/1‑149 (MQ=255)
                 gCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGc                                                                                                                            <  4:236849/149‑1 (MQ=255)
                     aaaaTAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCaga                                                                                                                        >  2:10081/1‑149 (MQ=255)
                         tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAgctg                                                                                                                    <  2:324571/149‑1 (MQ=255)
                                attcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGc                                                                                                             <  1:519482/149‑1 (MQ=255)
                                 ttcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCt                                                                                                            >  3:567255/1‑149 (MQ=255)
                                 ttcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCCTCtt                                                                                                                                           <  3:353504/118‑1 (MQ=255)
                                 ttcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCCTCtt                                                                                                                                           >  4:353504/1‑118 (MQ=255)
                                  tcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtt                                                                                                           <  3:2496/149‑1 (MQ=255)
                                   cattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttt                                                                                                          <  1:620456/149‑1 (MQ=255)
                                         ttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAc                                                                                                    >  3:641441/1‑149 (MQ=255)
                                         ttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAc                                                                                                    >  3:429113/1‑149 (MQ=255)
                                                     ttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGaa                                                                                        <  3:142847/149‑1 (MQ=255)
                                                        gTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt                                                                                           <  1:673892/143‑1 (MQ=255)
                                                        gTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt                                                                                           >  2:673892/1‑143 (MQ=255)
                                                         tACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct                                                                                                                                            <  2:330772/93‑1 (MQ=255)
                                                         tACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct                                                                                                                                            >  1:330772/1‑93 (MQ=255)
                                                         tACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCa                                                                                                                          <  1:690516/111‑1 (MQ=255)
                                                         tACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCa                                                                                                                          >  2:690516/1‑111 (MQ=255)
                                                           ctctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTAc                                                                                  >  4:210369/1‑149 (MQ=255)
                                                              ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑c                                                                                                                                                   <  1:416504/81‑1 (MQ=255)
                                                              ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑c                                                                                                                                                   >  2:416504/1‑81 (MQ=255)
                                                              ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                               <  1:160886/149‑1 (MQ=255)
                                                              ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                               >  3:555938/1‑149 (MQ=255)
                                                              ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                               >  2:358318/1‑149 (MQ=255)
                                                                cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                             <  2:421793/149‑1 (MQ=255)
                                                                cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                             >  2:581564/1‑149 (MQ=255)
                                                                 aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            >  3:281807/1‑149 (MQ=255)
                                                                 aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            >  2:672502/1‑149 (MQ=255)
                                                                 aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            >  2:244727/1‑149 (MQ=255)
                                                                        cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATa                                                                       <  1:233929/147‑1 (MQ=255)
                                                                        cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATa                                                                       >  2:233929/1‑147 (MQ=255)
                                                                        cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACt                                                                     >  1:507435/1‑149 (MQ=255)
                                                                          ttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGc                                                                   <  4:483109/149‑1 (MQ=255)
                                                                          ttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGc                                                                   <  3:72303/149‑1 (MQ=255)
                                                                            aTTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGa                                                                 <  3:246264/149‑1 (MQ=255)
                                                                                aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAAc                                                                                       <  2:509341/123‑1 (MQ=255)
                                                                                aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAAc                                                                                       >  1:509341/1‑123 (MQ=255)
                                                                                  aaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACCGCGATATCAg                                                           <  1:10081/149‑1 (MQ=255)
                                                                                         cGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGAt                                                    >  4:11495/1‑149 (MQ=255)
                                                                                             gATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTa                                                <  3:270251/149‑1 (MQ=255)
                                                                                               tAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACg                                              >  4:40836/1‑149 (MQ=255)
                                                                                                aaaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTa                                                >  4:570320/1‑146 (MQ=255)
                                                                                                aaaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTa                                                <  3:570320/146‑1 (MQ=255)
                                                                                                  aaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGaa                                                                         >  1:115988/1‑119 (MQ=255)
                                                                                                  aaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGaa                                                                         <  2:115988/119‑1 (MQ=255)
                                                                                                    gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGa                                         <  1:581564/149‑1 (MQ=255)
                                                                                                    gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGa                                         <  4:567255/149‑1 (MQ=255)
                                                                                                    gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGa                                         >  2:201285/1‑149 (MQ=255)
                                                                                                     tCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAt                                        >  3:522633/1‑149 (MQ=255)
                                                                                                     tCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAt                                        <  1:358318/149‑1 (MQ=255)
                                                                                                       aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGc                                                                                     <  2:26924/102‑1 (MQ=255)
                                                                                                       aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGc                                                                                     >  1:26924/1‑102 (MQ=255)
                                                                                                       aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAttt                                      >  2:620152/1‑149 (MQ=255)
                                                                                                        aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTc                                     >  2:388415/1‑149 (MQ=255)
                                                                                                           ttttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc                                  >  4:528394/1‑149 (MQ=255)
                                                                                                            tttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACa                                 <  4:522633/149‑1 (MQ=255)
                                                                                                              tGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               <  1:620152/149‑1 (MQ=255)
                                                                                                               gATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACagag                              <  3:11495/149‑1 (MQ=255)
                                                                                                               gATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACagag                              <  4:555938/149‑1 (MQ=255)
                                                                                                                 tGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGc                            >  4:461959/1‑149 (MQ=255)
                                                                                                                         aaaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCa                    >  4:567487/1‑149 (MQ=255)
                                                                                                                           aCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg                  >  4:110956/1‑149 (MQ=255)
                                                                                                                           aCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg                  >  1:223045/1‑149 (MQ=255)
                                                                                                                           aCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg                  <  1:388415/149‑1 (MQ=255)
                                                                                                                             gAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               >  1:175640/1‑134 (MQ=255)
                                                                                                                             gAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               <  2:175640/134‑1 (MQ=255)
                                                                                                                               aaaGAGGGAGACTAG‑CTCCCTCTTTCAACTGGGTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTATTCAGGCCAt              >  4:591176/1‑149 (MQ=255)
                                                                                                                                 agagGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtg            >  1:361122/1‑149 (MQ=255)
                                                                                                                                 agagGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtg            >  3:317981/1‑149 (MQ=255)
                                                                                                                                 agagGGAG‑CTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtg            >  3:247488/1‑149 (MQ=255)
                                                                                                                                  gagGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtga           >  3:660390/1‑149 (MQ=255)
                                                                                                                                   agGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTTAACAGAGGCTTTTTTCAGGCCATtgat          <  3:461959/149‑1 (MQ=255)
                                                                                                                                   agGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATagat          >  2:572838/1‑149 (MQ=255)
                                                                                                                                     ggAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               >  2:491406/1‑126 (MQ=255)
                                                                                                                                     ggAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               <  1:491406/126‑1 (MQ=255)
                                                                                                                                     ggAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtgatga        >  4:537926/1‑149 (MQ=255)
                                                                                                                                           tAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGc  <  1:244727/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGC  >  NC_000913/1799233‑1799520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A