Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F280 I0 R1 19 67.2 1649126 86.4% 1424844 237.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,613 Δ1 bp 7.5% intergenic (+100/‑5) fliK → / → fliL flagellar hook‑length control protein/flagellar biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,6130A. 7.5% 174.4 / 6.8 53intergenic (+100/‑5)fliK/fliLflagellar hook‑length control protein/flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base . (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

AAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGCCTGAAAGATGAAGCTACCCGC  >  NC_000913/2019336‑2019878
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                          
aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCAGCGTCTTTTCCCCGCTTTGTTGCGCGCAAGACGCCGGATAATTAGCGGCCAAGCAGTAGAGACACAGGAAAACAGCaa                                                                                                                                                                                                                                                                           >  1:619/1‑279 (MQ=255)
                  aaGCTTTAGTGGGCAGCAGAAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGGCAGAGGTAGCACCGTAATCCGCGGCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGAGAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGAt                                                                                                                                                                                                                                                               <  2:564909/273‑1 (MQ=255)
                                                        agcaaAGACAACGCACAGCAAACCAGGAGCCTCTGTCGTGGGAAGACGACGATACGCTTCCGGTTCCAGTCTCTTTACAAGGGCGTGGAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATcc                                                                                                                                                                                                                       <  2:43290/275‑1 (MQ=255)
                                                         gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCCGGATAATTAGCCGCATAGCAGTAGCGACACAGGAAGACCGCAAAACATGACTGATTACGCGATAAGCACAAAAAGCAAGCGAACGCTTTGAAcccc                                                                                                                                                                                                                      >  4:209260/1‑275 (MQ=255)
                                                                   tgCACAGCAAACCATGAACCTCTGGCGGGGGAAGACAACGCTACGCTTCCGGTTCCCGTCTCTTTCCAATGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTa                                                                                                                                                                                                            <  2:589441/274‑1 (MQ=255)
                                                                   cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTgg                                                                                                                                                                                                              <  2:433702/273‑1 (MQ=255)
                                                                                  gAACCTCTGCCGGGAGAAGACGACGATACGCTTCCGGTTCCCGTCTCCTTCCAAGTGCGTGTACCAGGCAAAAGCGGTGATGAGACTATCGCCCTACGTCAGCGGTAGCACCGTAATCCCCGTCTTTTCCTCGCTTTTTCCCACACGAGTCGCCCGACAATTAACCGGTTGTCAGTAGCGACAGTGGGAGGCCGCAAAGCATAACTGATGACGCAAGAAGTCAGAAAAGCAAGCGATCGCGTTGCATCCAGATACGGCTATTCATTACTCgcgca                                                                                                                                                                                             >  4:308093/1‑274 (MQ=255)
                                                                                           gCGGGGGAAGACGACGTTAGGCCTCCGTTTCCAGTCTCTTTACAAGGGCGTGTAACGGGAAACAGCCGCGTTGATAATTTCGCCTAACGTCAGAGGTAGAACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAAACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcg                                                                                                                                                                                <  1:187216/279‑1 (MQ=255)
                                                                                           gCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgc                                                                                                                                                                                 >  1:83408/1‑278 (MQ=255)
                                                                                           gCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAGCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcg                                                                                                                                                                                <  1:165404/279‑1 (MQ=255)
                                                                                                      taacggacacctcccGTTTCCCGTCTCTTTCAAAGGCGGTGTAACAGGCAACTGCGGCTTTGATATTTTCTCCTAACGTCAGAGGTCGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTa                                                                                                                                                                         <  4:501806/262‑1 (MQ=255)
                                                                                                                gtataatagacagTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCTTTGATATTTTCGCCTAACGTCAGAGGTAGAACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAt                                                                                                                                                            <  1:136166/266‑1 (MQ=255)
                                                                                                                      gTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAtt                                                                                                                                                           >  2:304645/1‑273 (MQ=255)
                                                                                                                           cGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAg                                                                                                                                                                             >  2:501806/1‑250 (MQ=255)
                                                                                                                                 tttACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGAGCGCTTTGGATCCCGATTCTGGTATTCATTACCCCCGCGGCCTGTGCCAGGCCGGCGTACAGCGCCTGGCCTTccccccacgtttc                                                                                                                                              >  4:299491/1‑263 (MQ=255)
                                                                                                                                         ggCGTTTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGACGCATAATTAGCCGATAACAAGTAGCGACACCTGAAGACCGAAACACCTCACTGATTCCGCGATAAGCAAGACAAGCAATCTCTCGGTTTGGATCCCGATTCCTGTATTCATTACCCTCGCGGACTGTTCCACCGCAGGTTAAAGCTATTAGCATTCGCATCCGGTTGCCGCTgcc                                                                                                                                       >  4:216735/1‑272 (MQ=255)
                                                                                                                                           cGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCCGGTTACCGCTAATGGCATTCGCATCCGGTTTCCCCTgacga                                                                                                                                     >  4:454804/1‑274 (MQ=255)
                                                                                                                                           cGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTgacga                                                                                                                                     >  2:47943/1‑274 (MQ=255)
                                                                                                                                             tgtAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTg                                                                                                                                                                                                                                                                 >  4:475648/1‑148 (MQ=255)
                                                                                                                                             tgtAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCGGACGACAAAgc                                                                                                                               >  1:151384/1‑278 (MQ=255)
                                                                                                                                             tgcaaCAGGCAACAGCGGCGTTGATATTTTCGCCTAACGCCAGAGGTAGCACCGTAACTCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGTTAATTAGCCGCTAAGCAGTAGCGACACAGGAAGACCGCAACACATGACCGATTACGCGTTAAGCAAGAAACGCAAGCAATCCCTTGTGATCCCGAGCCTGGTATTCATTACCCCCGCGGCCTGTGCCAGCCCAGGGTACACCTTCTGGCTTTCGTATCACGTTCTCGCTtacgac                                                                                                                                    >  4:184827/4‑273 (MQ=255)
                                                                                                                                                 aCAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTa                                                                                                                                                                                                                                                             <  2:475648/148‑1 (MQ=255)
                                                                                                                                                            cggcgTTGATATTTTCGCCTAACGTCCGAGGTAGCACCGTAATCCGCGTCTTTTACCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGAACGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGACGGTTACCACTACTGGCATTCGCATCCGGTTGCCGCGGAAGACAAAGCCAAGNaaaccgc                                                                                                                   >  4:562017/1‑270 (MQ=255)
                                                                                                                                                                                aaCGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGtcttct                                                                                               <  2:209260/275‑1 (MQ=255)
                                                                                                                                                                                     cAGAGGATGAACAGTAACCCGCGGCTTTTCCCCGCTTTGTTGCGCTAAAGAAGCAGGAAAATTAGCGGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGGGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTAcgcg                                                                                          <  4:304645/275‑1 (MQ=255)
                                                                                                                                                                                           tAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCt                                                                               >  1:61898/1‑279 (MQ=255)
                                                                                                                                                                                             gCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTg                                                                                                                                                                                                               >  1:34702/1‑150 (MQ=255)
                                                                                                                                                                                             gCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTg                                                                                                                                                                                                               <  3:34702/150‑1 (MQ=255)
                                                                                                                                                                                                   tAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCTGATAAGCAGTAGCGACACAGGAAGACGGCAACACATCACTGATTACTCGATAAGCCCGAAAAGCAAGCTATAGCCGTCGGACGCGATTCTGTTATTCATTACCCCCGCGGCCTGTCCCAGCTCAGGTTACAGCTACTCGCACTCGCTTCTGGTTGCCGCTGACGACACAGCCCAGCAACGCGTCGTTCACCCACGGTTCTACTACCCT‑CTGGACACCtacgc                                                                           >  4:486314/1‑271 (MQ=255)
                                                                                                                                                                                                    aaTCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCCTGCCCTCACCGGGCCTCCACGCG‑CTGGATAcccttgcc                                                                          >  4:201254/1‑269 (MQ=255)
                                                                                                                                                                                                        cgcgTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTAcg                                                                                            <  2:201254/254‑1 (MQ=255)
                                                                                                                                                                                                        cgcgTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCAAGCGCAGGTTACAGCTAATGGCATTGGCATCAGGATGCCGCTGACCACAACGGGCAGCAACGCGTCGCGCACCCACCGGTCTTATACCCC‑CGGGATACCttacaggtac                                                                      >  4:530546/1‑267 (MQ=255)
                                                                                                                                                                                                          cgTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAaagcaag                                                                                                                                                                                                                                       >  2:111322/1‑113 (MQ=255)
                                                                                                                                                                                                           gTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTa                                                                                                                                                                                                     <  1:32977/146‑1 (MQ=255)
                                                                                                                                                                                                           gTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTa                                                                                                                                                                                                     >  3:32977/1‑146 (MQ=255)
                                                                                                                                                                                                           gTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCCCGGCCTGAGCCAGCGCAGGTTACAGCTACCGGCATTACCATCAGGTTGCCGCTGCCGGCAAAGCGCCGCAACGCATGGTGCCCCCACCGGTCTTCCACCAG‑CTGGCTACCTTCACGGCCAAtt                                                                   >  4:357802/1‑275 (MQ=255)
                                                                                                                                                                                                              ttttCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGAt                                                                                                                                                                                                                                   <  4:111322/113‑1 (MQ=255)
                                                                                                                                                                                                                ttCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTg                                                                                                                                                                >  4:212695/1‑178 (MQ=255)
                                                                                                                                                                                                                    ccGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAt                                                                                                                                                            <  2:212695/178‑1 (MQ=255)
                                                                                                                                                                                                                       cTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGAACCCGATTCTGGCATTCATTACCCTCGCGGCCTGGGCCAGCGCAGGTTACAGCTACTGGCATTCGCGACAGGGTGCACCGGACGGAAAACCGCACGCACGCCGCGTTCCCCCCCCGGTCTTC‑ACCCGCCTTAATCACTTCACGGTCAATTTGGGCGAtaccg                                                       >  4:379185/1‑271 (MQ=255)
                                                                                                                                                                                                                        tnttttgAGCACAAAACTCAGAAAAAATAGGCANNAAGCAGCAAGAACACAGGAAGACCCAAACANATGACTGATTACGCGATAAGAAAAAAAAGCAACCGATCTCTTTGGATCCCGATTCTGGTATTCATTACCCTCGGNGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCATGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgc                                                  <  3:67899/275‑1 (MQ=255)
                                                                                                                                                                                                                         ttgttgCGCTCAAAACGCAGGATAATTAGCCGATAAGCAGTGGCGAAACAGGAAGACCGCAACACATGACTGATTACCCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgcg                                                 <  1:106238/279‑1 (MQ=255)
                                                                                                                                                                                                                             tgCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAATACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgcg                                                 <  2:562017/275‑1 (MQ=255)
                                                                                                                                                                                                                             tgCGCTCAAGACGAAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgcg                                                 <  2:5912/275‑1 (MQ=255)
                                                                                                                                                                                                                                      gACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACAGTACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTtata                                        <  2:66643/275‑1 (MQ=255)
                                                                                                                                                                                                                                       aCGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTatat                                       <  2:454804/275‑1 (MQ=255)
                                                                                                                                                                                                                                           aGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGACAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTTGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTACCCGGTCTTCTACGCG‑CTGGGTACCtt                                                                              >  4:353184/1‑231 (MQ=255)
                                                                                                                                                                                                                                           aGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCtt                                                                              >  4:340829/1‑231 (MQ=255)
                                                                                                                                                                                                                                               tAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACg                                                                          <  2:353184/231‑1 (MQ=255)
                                                                                                                                                                                                                                               tAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACg                                                                          <  2:340829/231‑1 (MQ=255)
                                                                                                                                                                                                                                                aaTTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGGATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTg                          <  1:104272/279‑1 (MQ=255)
                                                                                                                                                                                                                                                       cGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGGCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTgc                         >  2:72962/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                          aGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTAACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGCCTGAAAGATGAAGCTAc      <  2:547507/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACACCTACTGGCATTCGCATCCGGTTGCCGCTGACGCCAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCCCGGTCAATTTGGGCGATCCGGATGCCGTTGTTTATATCGGCATACCCCTGCGCCGAAAAAATGAAGCCACCCGc  >  4:332865/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                          
AAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGCCTGAAAGATGAAGCTACCCGC  >  NC_000913/2019336‑2019878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A