Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F195 I0 R2 483 52.1 1908604 98.1% 1872340 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,537,165 G→A 6.1% L49L (CTG→TTG)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,537,1650GA 6.1% 210.1 / 2.3 65L49L (CTG→TTG) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCATTA  >  NC_000913/1537017‑1537311
                                                                                                                                                    |                                                                                                                                                  
aGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATTcag                                                                                                                                                    >  2:142012/1‑149 (MQ=255)
aGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCcag                                                                                                                                                    >  2:385643/1‑149 (MQ=255)
    gCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCagcagc                                                                                                                                                >  3:90754/1‑149 (MQ=255)
                       cTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTtgt                                                                                                                             <  4:90754/149‑1 (MQ=255)
                        tCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTtgtg                                                                                                                            >  2:305315/1‑149 (MQ=255)
                        tCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTtgtg                                                                                                                            >  4:217243/1‑149 (MQ=255)
                         ccGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTgg                                                                                                                           <  4:273912/149‑1 (MQ=255)
                         ccGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTgg                                                                                                                           <  3:217243/149‑1 (MQ=255)
                         ccGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTgg                                                                                                                           <  3:306536/149‑1 (MQ=255)
                               gcagGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGaaa                                                                                                                     <  1:208971/149‑1 (MQ=255)
                               gcagGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGaaa                                                                                                                     >  1:459482/1‑149 (MQ=255)
                               gcagGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGaaa                                                                                                                     <  4:373834/149‑1 (MQ=255)
                                 agGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTgg                                                                                                                           >  1:217601/1‑141 (MQ=255)
                                 agGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTgg                                                                                                                           <  2:217601/141‑1 (MQ=255)
                                      caccaTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTaa                                                                                                              <  2:306459/149‑1 (MQ=255)
                                      caccaTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGCTTATCCAGCAGCGGCGCGTTAAGCAGTTTGTGGTTAAAATCCGTaa                                                                                                              <  4:214953/149‑1 (MQ=255)
                                         caTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTaa                                                                                                              <  3:45028/146‑1 (MQ=255)
                                         caTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTaa                                                                                                              >  4:45028/1‑146 (MQ=255)
                                         caTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCa                                                                                                           >  3:436310/1‑149 (MQ=255)
                                         caTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCa                                                                                                           >  4:152304/1‑149 (MQ=255)
                                         caTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAACAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCa                                                                                                           >  2:87800/1‑149 (MQ=255)
                                           ttGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATc                                                                                                         >  2:433787/1‑149 (MQ=255)
                                           ttGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATc                                                                                                         >  1:115261/1‑149 (MQ=255)
                                                tGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGgcgc                                                                                                    >  4:451998/1‑149 (MQ=255)
                                                     gcgATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTcgc                                                                                               >  2:323342/1‑149 (MQ=255)
                                                             gggACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATc                                                                                       <  4:322597/149‑1 (MQ=255)
                                                                aCTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAAc                                                                                    <  1:433787/149‑1 (MQ=255)
                                                                aCTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAACCCGTAAGCATCGGCGCGTCGCGGCGGATCAAc                                                                                    <  3:372672/149‑1 (MQ=255)
                                                                  tCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGc                                                                                  >  3:83141/1‑149 (MQ=255)
                                                                      cATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgc                                                                              <  2:115261/149‑1 (MQ=255)
                                                                      cATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgc                                                                              <  4:321651/149‑1 (MQ=255)
                                                                      cATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAACAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgc                                                                              >  4:221810/1‑149 (MQ=255)
                                                                       aTGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAAc                                                                                    <  2:126153/142‑1 (MQ=255)
                                                                       aTGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAAc                                                                                    >  1:126153/1‑142 (MQ=255)
                                                                       aTGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgcg                                                                             >  1:78926/1‑149 (MQ=255)
                                                                             aTGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGtcct                                                                       >  1:344500/1‑149 (MQ=255)
                                                                                ttCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTa                                                                                                               <  2:464461/106‑1 (MQ=255)
                                                                                ttCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTa                                                                                                               >  1:464461/1‑106 (MQ=255)
                                                                                 tCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCtt                                                                   >  2:52071/1‑149 (MQ=255)
                                                                                    aaCAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCa                                                                >  1:304983/1‑149 (MQ=255)
                                                                                                       tgCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAACAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTcc                                             <  3:221810/149‑1 (MQ=255)
                                                                                                           cccGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCAGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGAt                                         >  4:414511/1‑149 (MQ=255)
                                                                                                            ccGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATa                                        >  2:363629/1‑149 (MQ=255)
                                                                                                                  tCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCAt                                  <  2:78926/149‑1 (MQ=255)
                                                                                                                    aaaCACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCa                                >  1:471220/1‑149 (MQ=255)
                                                                                                                    aaaCACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCa                                >  2:379775/1‑149 (MQ=255)
                                                                                                                      acacTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAAc                              <  2:471220/149‑1 (MQ=255)
                                                                                                                      acacTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAAc                              >  1:284166/1‑149 (MQ=255)
                                                                                                                         cTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAgg                           >  4:413732/1‑149 (MQ=255)
                                                                                                                          ttCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGc                          >  2:153634/1‑149 (MQ=255)
                                                                                                                          ttCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGc                          >  4:174679/1‑149 (MQ=255)
                                                                                                                          ttCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGc                          <  1:153634/149‑1 (MQ=255)
                                                                                                                             gCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGa                       <  3:152304/149‑1 (MQ=255)
                                                                                                                              cACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGAt                      <  1:323342/149‑1 (MQ=255)
                                                                                                                                 cATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCAc                   >  1:457993/1‑149 (MQ=255)
                                                                                                                                      ggCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGa              <  3:451998/149‑1 (MQ=255)
                                                                                                                                      ggCCTGTTTATCCAACAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGa              <  1:87800/149‑1 (MQ=255)
                                                                                                                                         cTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCAt                                  >  3:252757/1‑126 (MQ=255)
                                                                                                                                         cTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCAt                                  <  4:252757/126‑1 (MQ=255)
                                                                                                                                           gTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATc         >  1:180424/1‑149 (MQ=255)
                                                                                                                                            tttATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCt        >  3:174175/1‑149 (MQ=255)
                                                                                                                                              tATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGc      <  4:436310/149‑1 (MQ=255)
                                                                                                                                               aTCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGa              <  1:54773/140‑1 (MQ=255)
                                                                                                                                               aTCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGa              >  2:54773/1‑140 (MQ=255)
                                                                                                                                               aTCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCa     <  1:31674/149‑1 (MQ=255)
                                                                                                                                               aTCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCa     <  3:414511/149‑1 (MQ=255)
                                                                                                                                                tCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCat    >  1:104395/1‑149 (MQ=255)
                                                                                                                                                 ccAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCatt   <  2:104395/149‑1 (MQ=255)
                                                                                                                                                  cagcagCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCatta  >  2:136602/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATCAACAGGCCGATCACTTTGAGGATCTGCATTA  >  NC_000913/1537017‑1537311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A