Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F195 I0 R2 483 52.1 1908604 98.1% 1872340 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,904,592 C→A 9.8% T233N (ACT→AAT)  manZ → mannose‑specific enzyme IID component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,904,5920CA 9.8% 205.8 / 11.5 61T233N (ACT→AAT) manZmannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base A (2/4);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

TTCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGA  >  NC_000913/1904444‑1904740
                                                                                                                                                     |                                                                                                                                                    
ttCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGAc                                                                                                                                                      >  2:234330/1‑149 (MQ=255)
       aaaaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTg                                                                                                                                               <  3:63015/149‑1 (MQ=255)
       aaaaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTg                                                                                                                                               <  1:450773/149‑1 (MQ=255)
       aaaaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTg                                                                                                                                               <  1:39372/149‑1 (MQ=255)
          aaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGAATAGTCTGGAc                                                                                                                                            <  1:332104/149‑1 (MQ=255)
           aCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGGTCCAGAATAGTCTGGAc                                                                                                                                            <  4:65417/149‑1 (MQ=255)
           aCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGAcc                                                                                                                                           >  1:153245/1‑149 (MQ=255)
                 ggAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAg                                                                                                                                         <  3:243829/145‑1 (MQ=255)
                 ggAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAg                                                                                                                                         >  4:243829/1‑145 (MQ=255)
                      gggCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCa                                                                                                                                <  3:315511/149‑1 (MQ=255)
                      gggCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCa                                                                                                                                <  4:189287/149‑1 (MQ=255)
                       ggCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAg                                                                                                                               <  3:323363/149‑1 (MQ=255)
                       ggCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAg                                                                                                                               <  2:153245/149‑1 (MQ=255)
                               tCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTa                                                                                                                       >  2:77588/1‑149 (MQ=255)
                                 cTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGAATAGTCTGGa                                                                                                                                             <  3:112750/125‑1 (MQ=255)
                                 cTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACGGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGAATAGTCTGGa                                                                                                                                             >  4:112750/1‑125 (MQ=255)
                                       cTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGAATAGTCTGGAcagta                                                                                                                                        <  3:253840/124‑5 (MQ=255)
                                       cTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGAATAGTCTGGAcagta                                                                                                                                        >  4:253840/1‑120 (MQ=255)
                                           ttGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCActgctgctg                                                                                                           <  2:425689/149‑1 (MQ=255)
                                                        aTTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTa                                                                                              >  1:297211/1‑149 (MQ=255)
                                                        aTTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTa                                                                                              <  1:153088/149‑1 (MQ=255)
                                                        aTTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTa                                                                                              >  3:454258/1‑149 (MQ=255)
                                                           ggTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGc                                                                                                                                  >  4:78347/1‑110 (MQ=255)
                                                           ggTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGc                                                                                                                                  <  3:78347/110‑1 (MQ=255)
                                                                  aaGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCt                                                                                                                                                >  2:251166/1‑89 (MQ=255)
                                                                  aaGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCt                                                                                                                                                <  1:251166/89‑1 (MQ=255)
                                                                  aaGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACt                                                                                    >  1:337352/1‑149 (MQ=255)
                                                                            aTGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCaaaaaa                                                                          <  4:189737/149‑1 (MQ=255)
                                                                                cAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTa                                                                      <  1:234330/149‑1 (MQ=255)
                                                                                   cATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAcc                                                                   >  2:240022/1‑149 (MQ=255)
                                                                                       ccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCActgctgct                                                                                                            <  3:282959/104‑1 (MQ=255)
                                                                                       ccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCActgctgct                                                                                                            >  4:282959/1‑104 (MQ=255)
                                                                                        cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCtg                                                              >  2:172478/1‑149 (MQ=255)
                                                                                            ggTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGa                                                          <  2:35317/149‑1 (MQ=255)
                                                                                             gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtgt                                                                                           <  2:230716/115‑1 (MQ=255)
                                                                                             gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtgt                                                                                           >  1:230716/1‑115 (MQ=255)
                                                                                               tGTCTCTCGCATTACTGACCAGACGGGCAAAGAACTCGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGTCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTTGatca                                                       <  1:12808/149‑1 (MQ=255)
                                                                                               tGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatca                                                       <  3:164416/149‑1 (MQ=255)
                                                                                                 tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCAct                                                                                                                  >  1:293652/1‑88 (MQ=255)
                                                                                                 tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCAct                                                                                                                  <  2:293652/88‑1 (MQ=255)
                                                                                                 tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatcatc                                                     <  4:454258/149‑1 (MQ=255)
                                                                                                 tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatcatc                                                     <  2:297211/149‑1 (MQ=255)
                                                                                                   tctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCtt                                                                                                 >  1:121052/1‑103 (MQ=255)
                                                                                                   tctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCtt                                                                                                 <  2:121052/103‑1 (MQ=255)
                                                                                                   tctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGt                                                   >  4:91372/1‑149 (MQ=255)
                                                                                                             cTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCttcttc                                         >  1:475061/1‑149 (MQ=255)
                                                                                                              tGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGATCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTTCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatc                                                        <  2:49953/133‑1 (MQ=255)
                                                                                                              tGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatc                                                        >  1:49953/1‑133 (MQ=255)
                                                                                                                 ccAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCa                                     <  3:12508/149‑1 (MQ=255)
                                                                                                                        ggCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCttcttc                                         >  2:148265/1‑138 (MQ=255)
                                                                                                                        ggCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCttcttc                                         <  1:148265/138‑1 (MQ=255)
                                                                                                                           aaaGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGc                           >  1:163445/1‑149 (MQ=255)
                                                                                                                              gAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTgg                        <  2:337352/149‑1 (MQ=255)
                                                                                                                                 cacGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTa                     >  2:52525/1‑149 (MQ=255)
                                                                                                                                      tactacT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt                       <  2:464709/142‑1 (MQ=255)
                                                                                                                                      tactacT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt                       >  1:464709/1‑142 (MQ=255)
                                                                                                                                         tact‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGtt                      <  4:218041/140‑1 (MQ=255)
                                                                                                                                         tact‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGtt                      >  3:218041/1‑140 (MQ=255)
                                                                                                                                         tact‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCg             >  1:21758/1‑149 (MQ=255)
                                                                                                                                               tCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCctg        >  3:223765/1‑149 (MQ=255)
                                                                                                                                                   gACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGcc          <  4:15632/143‑1 (MQ=255)
                                                                                                                                                   gACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGcc          >  3:15632/1‑143 (MQ=255)
                                                                                                                                                     cTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGa  <  1:252162/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACT‑GTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGA  >  NC_000913/1904444‑1904740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A