Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A51 F1 I1 R1
|
20 |
58.0 |
3778966 |
96.2% |
3635365 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,108,409 |
T→C |
K32E (AAG→GAG) |
metJ ← |
transcriptional repressor protein MetJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,108,409 | 0 | T | C | 95.7%
| 274.8
/ 0.6
| 69 | K32E (AAG→GAG) | metJ | transcriptional repressor protein MetJ |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (31/35); total (31/38) |
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATA > REL606/4108336‑4108479
|
tCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCaa < 1:1695581/76‑1 (MQ=255)
tCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCaa < 1:1390642/76‑1 (MQ=255)
cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAag > 2:1458643/1‑76 (MQ=255)
cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAag < 2:1819120/76‑1 (MQ=255)
cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAag > 2:1740359/1‑76 (MQ=255)
cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTT‑ATCGGTGAGGATTTTTAACACCTCAaga < 1:724212/76‑1 (MQ=255)
tGTTGGTATCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAgg > 1:495482/1‑76 (MQ=255)
ngTTGGTATCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAgg > 1:110525/2‑76 (MQ=255)
gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGa < 1:1067199/76‑1 (MQ=255)
ttGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGaa > 2:1572340/1‑76 (MQ=255)
tGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAAt < 1:505196/76‑1 (MQ=255)
gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATgg < 1:842911/76‑1 (MQ=255)
gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATgg < 1:1212055/76‑1 (MQ=255)
gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATgg < 2:213376/76‑1 (MQ=255)
gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATgg < 1:1484735/76‑1 (MQ=255)
tAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGa > 1:1019985/1‑76 (MQ=255)
aGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGaa > 1:1215601/1‑76 (MQ=255)
aGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGaa > 2:308063/1‑76 (MQ=255)
cGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAAc > 2:1455336/1‑76 (MQ=255)
gTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAAcc < 2:77844/76‑1 (MQ=255)
aCGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTa < 2:1095718/76‑1 (MQ=255)
cGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTaa < 1:659084/76‑1 (MQ=255)
cGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTaa < 1:18462/76‑1 (MQ=255)
ncAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAAt > 1:896390/2‑76 (MQ=255)
aGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCt < 2:359982/76‑1 (MQ=255)
aGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCt > 2:323533/1‑76 (MQ=255)
gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttt > 1:1049242/1‑76 (MQ=255)
gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttt > 1:544903/1‑76 (MQ=255)
gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttt < 2:747678/76‑1 (MQ=255)
gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttt > 1:799798/1‑76 (MQ=255)
tgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttttt < 1:1134293/76‑1 (MQ=255)
tgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttttt < 1:898696/76‑1 (MQ=255)
tgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCttttt > 1:1407709/1‑76 (MQ=255)
gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTg < 2:687399/76‑1 (MQ=255)
gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTg > 1:291112/1‑76 (MQ=255)
ttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGa < 1:1454262/76‑1 (MQ=255)
ttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGa < 1:516797/76‑1 (MQ=255)
ccTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTg < 2:1407709/76‑1 (MQ=255)
tGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt > 2:564790/1‑76 (MQ=255)
tGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt > 1:1245366/1‑76 (MQ=255)
gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTc > 2:578843/1‑76 (MQ=255)
cgacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAc < 1:1521117/76‑1 (MQ=255)
acgCGTGCGTTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCActc > 1:842197/1‑76 (MQ=255)
gcgtgcgtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtctt < 2:486953/76‑1 (MQ=255)
gtgcgtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct < 2:873698/76‑1 (MQ=255)
gtgcgtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct > 1:55595/1‑76 (MQ=255)
gcgtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTg < 2:1115910/76‑1 (MQ=255)
cgtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGc > 2:350500/1‑76 (MQ=255)
cgtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGc < 1:242853/76‑1 (MQ=255)
gtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGcc < 2:1111795/76‑1 (MQ=255)
gtTCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGcc > 1:1471748/1‑76 (MQ=255)
ttCATCGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCg < 1:485690/76‑1 (MQ=255)
cGGTGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTc < 1:1045355/76‑1 (MQ=255)
tGAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGc < 2:48807/76‑1 (MQ=255)
gAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCg > 1:1775946/1‑76 (MQ=255)
gAGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCg > 1:1775940/1‑76 (MQ=255)
aGGATTTTTAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGt < 2:1562840/76‑1 (MQ=255)
tttttAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATgg < 1:6670/76‑1 (MQ=255)
tttttAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATgg < 1:224196/76‑1 (MQ=255)
ttttAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATggg > 1:1540483/1‑76 (MQ=255)
ttttAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATggg > 1:883999/1‑76 (MQ=255)
ttttAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATggg > 1:1434591/1‑76 (MQ=255)
tttAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGc > 1:824396/1‑76 (MQ=255)
tAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTg < 2:470171/76‑1 (MQ=255)
tAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTg < 2:671992/76‑1 (MQ=255)
tAACACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTg > 2:488127/1‑76 (MQ=255)
aaCACCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGa > 2:1881324/1‑76 (MQ=255)
acacCTCAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGat < 1:1261106/76‑1 (MQ=255)
cacCTCAAGAGGAATGGAAACCGTAATCGTTGTGACTTGTTCACTCTGCTTGCCGTGCTCAGCGTATGGGCTGata < 2:329375/76‑1 (MQ=255)
|
TCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATA > REL606/4108336‑4108479
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
