Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A137 F1 I1 R1
|
18 |
221.5 |
13936938 |
95.6% |
13323712 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,145,726 |
G→A |
intergenic (+57/‑138) |
dnaG → / → rpoD |
DNA primase/RNA polymerase sigma factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,145,726 | 0 | G | A | 99.2%
| 959.6
/ ‑1.7
| 234 | intergenic (+57/‑138) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (134/98); total (134/100) |
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 |
Only 100 of 242 total aligned reads displayed.
AGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAA > REL606/3145653‑3145798
|
aGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGc < 2:5255766/76‑1 (MQ=255)
gCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCa > 2:23468/1‑76 (MQ=255)
cTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAg > 1:3654595/1‑76 (MQ=255)
tGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAagcagc > 2:2400860/1‑76 (MQ=255)
tGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAagcagc > 2:4330530/1‑76 (MQ=255)
ggCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCg < 1:2468674/76‑1 (MQ=255)
ggCAAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCg < 2:2232647/76‑1 (MQ=255)
gCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCgg < 1:5328996/76‑1 (MQ=255)
gCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCgg < 1:4921734/76‑1 (MQ=255)
cGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGc > 2:3751243/1‑76 (MQ=255)
gAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCTGCACTGAGAAGCAGCGGCa < 1:62953/76‑1 (MQ=255)
gAAAAAGTGATTTAACGGCTAAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCa < 1:4543009/76‑1 (MQ=255)
aaaaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCaa < 1:2618604/76‑1 (MQ=255)
aaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAat > 2:6680016/1‑76 (MQ=255)
taGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAata > 2:3866981/2‑76 (MQ=255)
aGTGATTTAAGGGCTAAAGGGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAatat < 2:5458138/76‑1 (MQ=255)
aGTGATTTAACGGCTTAAGTGCCGAAGAGCGACCGAGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAatat < 1:4093456/76‑1 (MQ=255)
tGATTAAACGGCTTAGGTGCGGAAGAGCGATCGGAAA‑CCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAatatat < 2:2114596/76‑1 (MQ=255)
gATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAAtatata > 1:696293/1‑76 (MQ=255)
gATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAAtatata < 1:737694/76‑1 (MQ=255)
gATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAAtatata < 1:4218325/76‑1 (MQ=255)
aTTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCATTGAGAAGCAGCGGCAAATATATaa > 2:6844240/1‑76 (MQ=255)
tttAACGTCTAAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAg < 2:6427431/76‑1 (MQ=255)
tttAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAg < 1:5450898/76‑1 (MQ=255)
tttAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAg > 1:3587675/1‑76 (MQ=255)
ttAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGt > 1:3850608/1‑76 (MQ=255)
tAACGGATAAATTGCAGAAACGCGCTCGGTAC‑TCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTa < 2:6812400/76‑1 (MQ=255)
aaCGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTAc > 1:2751892/1‑76 (MQ=255)
aCGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACg > 2:1875093/1‑76 (MQ=255)
gCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGccc > 2:900577/1‑76 (MQ=255)
cTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCt > 2:1555936/1‑76 (MQ=255)
cTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCt > 1:2517298/1‑76 (MQ=255)
ttAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTc > 2:1905724/1‑76 (MQ=255)
ttAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTc > 1:3156064/1‑76 (MQ=255)
ttAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTc > 2:1292321/1‑76 (MQ=255)
taaaGTCCGGAAGGGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTc < 2:6447251/74‑1 (MQ=255)
tAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCg > 2:3229957/1‑76 (MQ=255)
tAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCg > 1:986654/1‑76 (MQ=255)
tAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCg > 1:2159087/1‑76 (MQ=255)
tAAGTGCAGAGGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCg < 1:815545/76‑1 (MQ=255)
aggTGCAGAAGAGCG‑TCGGGAATGCCCCCG‑CCGGCACACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTa < 2:3898179/74‑1 (MQ=255)
aaGTGCGGAAGAGGAACGGGGAC‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGt < 1:1162199/76‑1 (MQ=255)
aaGTGCGAAAGAGGGATCGGAAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGt < 2:297911/76‑1 (MQ=255)
aaGTGCCGAAGGCCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGt < 2:4733920/76‑1 (MQ=255)
aaGTCCCGAAGAGGGACCGGAAA‑GCCCCCGCCAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGt < 2:1233585/76‑1 (MQ=255)
aGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTa > 2:6550324/1‑76 (MQ=255)
aGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTa > 2:5188603/1‑76 (MQ=255)
tGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAAt > 2:1715690/1‑76 (MQ=255)
ccGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTa > 2:294897/1‑76 (MQ=255)
ccGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTa > 1:3794121/1‑76 (MQ=255)
ccGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTa > 2:1348692/1‑76 (MQ=255)
gAAGAGCGATGGGGAC‑CCCCCCGCCAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATc < 2:59180/76‑1 (MQ=255)
gAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATc > 1:5226278/1‑76 (MQ=255)
aaGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCg > 1:6608471/1‑76 (MQ=255)
gagCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGtt > 2:3595482/1‑76 (MQ=255)
gagCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGtt > 2:4630430/1‑76 (MQ=255)
ggCGAGCGGAAA‑CCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTg < 2:5093544/75‑1 (MQ=255)
cGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGc > 1:4697528/1‑76 (MQ=255)
gATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCg > 2:5302721/1‑76 (MQ=255)
gATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCg > 2:5155540/1‑76 (MQ=255)
gATCGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCg > 1:908972/1‑76 (MQ=255)
ggggAA‑CCCCCCGCCAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTa < 2:6177199/75‑1 (MQ=255)
cGGGAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTa > 2:6231894/1‑76 (MQ=255)
gggAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTaa > 2:5610310/1‑76 (MQ=255)
gggAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTaa > 1:3500038/1‑76 (MQ=255)
gAA‑GCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaac > 2:330442/1‑76 (MQ=255)
gCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaacaac < 1:5055995/76‑1 (MQ=255)
gCCCCCGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaacaac < 1:5013474/76‑1 (MQ=255)
ccccGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCg > 1:4332200/1‑76 (MQ=255)
ccccGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCg > 2:6044137/1‑76 (MQ=255)
cccGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGt > 2:4876281/1‑76 (MQ=255)
cggACAGCCGCACTGAGAAGCAGCGCCAAATATATAAGTGCGCCCTCGTAATTATCGTTGGCGGTAAACAACCGtt < 2:3689646/74‑1 (MQ=255)
ccGACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGtt > 1:879582/1‑76 (MQ=255)
gccAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTgg < 1:6025628/74‑1 (MQ=255)
gccAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTgg < 2:4940395/74‑1 (MQ=255)
gACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTgg < 2:886253/76‑1 (MQ=255)
gACAGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTgg < 1:5966941/76‑1 (MQ=255)
cAGCCGCACTGAGAAGCAGCGGCAAATATATAAGAACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGAt > 2:312524/1‑76 (MQ=255)
aGCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGAtt > 2:6705943/1‑76 (MQ=255)
gCCGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGAttt < 1:1862497/76‑1 (MQ=255)
ccGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTc > 2:2455799/1‑76 (MQ=255)
tgCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCa > 2:352535/2‑76 (MQ=255)
cGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCa < 1:2799703/76‑1 (MQ=255)
cGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCa > 1:6405326/1‑76 (MQ=255)
cGCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCa > 2:6203499/1‑76 (MQ=255)
gCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAg < 2:4231183/76‑1 (MQ=255)
gCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAg < 1:1324664/76‑1 (MQ=255)
gCACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAg < 2:1115185/76‑1 (MQ=255)
cACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGc > 1:6213851/1‑76 (MQ=255)
cACTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGc > 2:3331298/1‑76 (MQ=255)
aCTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCg > 2:1798345/1‑76 (MQ=255)
aCTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCg > 1:6479872/1‑76 (MQ=255)
cTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGt > 2:1967199/1‑76 (MQ=255)
cTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGt > 1:6807559/1‑76 (MQ=255)
cTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGt > 2:5664617/1‑76 (MQ=255)
cTGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGt > 2:3926254/1‑76 (MQ=255)
tGAGAAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGtt > 1:3194452/1‑76 (MQ=255)
tGAGAAGCAGCGGCAAATATATAAGTACACCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGtt > 1:4941186/1‑76 (MQ=255)
gagaAGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTa > 2:3071978/1‑76 (MQ=255)
agaaGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTaa > 1:1410097/1‑76 (MQ=255)
|
AGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAA‑GCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAA > REL606/3145653‑3145798
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
