Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A137 F1 I1 R1
|
18 |
221.5 |
13936938 |
95.6% |
13323712 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,326,566 |
A→T |
D67E (GAT→GAA) |
mreD ← |
cell wall structural complex MreBCD transmembrane component MreD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,326,566 | 0 | A | T | 98.7%
| 619.2
/ ‑0.0
| 152 | D67E (GAT→GAA) | mreD | cell wall structural complex MreBCD transmembrane component MreD |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (101/49); total (102/50) |
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 |
Only 100 of 153 total aligned reads displayed.
ATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAAC > REL606/3326493‑3326638
|
aTTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTc > 2:4384868/1‑76 (MQ=255)
tttCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTcc > 2:4221630/1‑76 (MQ=255)
ttCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCa > 1:3538800/1‑76 (MQ=255)
tcggcgcCACCAGGAACGCAATGAGGCACATCGCAAATCCGGGTCCCCAAGGCGTGGAGCCGCTGATCAGTTCCAg < 2:2797052/73‑1 (MQ=255)
tCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAg > 1:3864669/1‑76 (MQ=255)
tCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAg > 2:6450068/1‑76 (MQ=255)
cAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGt > 2:2343803/1‑76 (MQ=255)
aGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTa > 1:5056147/1‑76 (MQ=255)
aGCGCCACCAGGAAAGAAAGAATGCTCATCGCCAATGCGCGTACGCCAAGCGGGGAGCCGCTGATCAGTTCCAGTa < 2:5441482/76‑1 (MQ=255)
ccccaccaGGTAAGCAATGATGCTCACCGCCAAGCCGCGCACGCCAAGCGGCGAGCCGCTGATCAGTTCCAGTATg < 1:3214774/74‑1 (MQ=255)
gcCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATgg > 1:4177636/1‑76 (MQ=255)
cccacaaGGTAAGCAAGGATGCCCACCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATgg < 1:1578188/75‑1 (MQ=255)
ccaccaGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGc > 2:4425415/1‑76 (MQ=255)
cacgaGGAAAGAAATGATCCTCACCGCAAATACGGGTACCCAAGGCGGGGAGCCGCTGATCAGTTCCAGTATGGCa < 2:4512353/76‑1 (MQ=255)
caccaGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCa > 2:4707386/1‑76 (MQ=255)
caccaGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCa > 1:5673387/1‑76 (MQ=255)
caccaGGAAAGCAATGTTGCCCATCGCCAATCCGCGTACCCAAAGCGGGGAGCCGCTGATCAGTTCCAGTATGGCa < 2:857731/76‑1 (MQ=255)
accaGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCAc > 2:2434254/1‑76 (MQ=255)
accaGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCAc > 1:6200149/1‑76 (MQ=255)
ccaGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCAcc > 2:6941105/1‑76 (MQ=255)
aGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCa > 1:5013436/1‑76 (MQ=255)
gTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATc > 1:1881698/1‑76 (MQ=255)
tAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGAACCCATca > 2:2185357/1‑76 (MQ=255)
tAAGAAATGAGCCGCACCCCCAATGCGCGTACGCCAGGCGGCGAGCCGCTGATCAGTTCCAGTATGGCACCCATca < 1:6899585/76‑1 (MQ=255)
gaaGAAATGATCCTCATCGCCAATCCCCGTACGCCAAGCGGGGAGCCGCTGATCAGTTCCAGTATGGCACCCATca < 2:6904446/75‑1 (MQ=255)
cAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACaaa > 1:4543525/1‑76 (MQ=255)
atggatgCTCAGCGCCAATCCGCGTCCGCCAGGCGTGGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACaaaa < 2:6255459/73‑1 (MQ=255)
aaTGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACaaaa > 2:1815449/1‑76 (MQ=255)
atgatgCTCATCGCCAATACGCGTACGCCAAGCGTCGGGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAAc > 2:6530002/1‑76 (MQ=255)
atgatgCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAAc > 1:6036042/1‑76 (MQ=255)
tgatgCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACGCATCACAAAAcc > 1:1745030/1‑76 (MQ=255)
tgatgCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAAcc > 1:3867841/1‑76 (MQ=255)
gCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCtgtg > 2:2340783/1‑76 (MQ=255)
cTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGc > 1:4928700/1‑76 (MQ=255)
tCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGcc > 2:5295158/1‑76 (MQ=255)
aTCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCca > 2:1726224/1‑76 (MQ=255)
aTCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCca > 1:4128792/1‑76 (MQ=255)
tCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCcac > 2:3590340/1‑76 (MQ=255)
tCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCcac > 2:623419/1‑76 (MQ=255)
cGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCcaca > 1:2527186/1‑76 (MQ=255)
cGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCcaca > 2:2999454/1‑76 (MQ=255)
ccaaaTCCGCGTACCCAAGGGGGCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACAt < 2:4250300/73‑1 (MQ=255)
caaaTGCGCGTCCCCAAAGCGGCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACAtt < 2:3956066/74‑1 (MQ=255)
cAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACAttt > 2:6559136/1‑76 (MQ=255)
cAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACAttt > 2:2055312/1‑76 (MQ=255)
cAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACAttt > 2:2424531/1‑76 (MQ=255)
cAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACAttt > 1:4862987/1‑76 (MQ=255)
aaTCCGCGGACGCCAGGGGTGGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTa < 1:3372694/76‑1 (MQ=255)
aaTCCGCGCACGCAAGGGGTGGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTa < 1:3540784/76‑1 (MQ=255)
aaTACGCGTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTa > 2:578616/1‑76 (MQ=255)
cgcgTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTAcgcg > 2:6942559/1‑76 (MQ=255)
cgcgTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTAcgcg > 2:2995297/1‑76 (MQ=255)
gcgTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGa > 2:1237633/1‑76 (MQ=255)
gcgTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGCATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGa > 1:742285/1‑76 (MQ=255)
gTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATg > 2:55982/1‑76 (MQ=255)
gTACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATg > 2:102439/1‑76 (MQ=255)
tACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGa > 2:4606898/1‑76 (MQ=255)
tACGCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGa > 1:6595556/1‑76 (MQ=255)
gCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGc > 1:1008598/1‑76 (MQ=255)
gCCAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGc > 1:6105901/1‑76 (MQ=255)
ccAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCa > 2:4991549/1‑76 (MQ=255)
cAAGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCaa > 1:3495850/1‑76 (MQ=255)
aaGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAg > 2:2661428/1‑76 (MQ=255)
aaGCGTCGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAg > 1:237431/1‑76 (MQ=255)
gCGTGGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGc < 2:4658567/76‑1 (MQ=255)
ggggAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCa < 2:6743943/73‑1 (MQ=255)
ggggAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCa < 2:3229007/73‑1 (MQ=255)
gcggAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCa < 1:1213936/73‑1 (MQ=255)
gggAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAg < 2:318313/74‑1 (MQ=255)
ggAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAgg < 1:2138038/75‑1 (MQ=255)
cGAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAgg > 2:5302780/1‑76 (MQ=255)
gAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGa > 1:1308729/1‑76 (MQ=255)
gAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGa > 1:2237889/1‑76 (MQ=255)
gAGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGa > 2:6890122/1‑76 (MQ=255)
aGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGAt < 1:5154273/76‑1 (MQ=255)
aGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGAt < 1:4654358/76‑1 (MQ=255)
aGCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGAt < 1:2340908/76‑1 (MQ=255)
gCCGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATc < 1:1698556/76‑1 (MQ=255)
ccGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATcc < 1:6450068/76‑1 (MQ=255)
ccGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATcc > 1:6817648/1‑76 (MQ=255)
cGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa > 2:5731917/1‑76 (MQ=255)
cGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa < 2:5089947/76‑1 (MQ=255)
cGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa < 2:158732/76‑1 (MQ=255)
cGCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa > 2:3965859/1‑76 (MQ=255)
gCTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCaa > 2:1390448/1‑76 (MQ=255)
cTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAAt > 2:5981403/1‑76 (MQ=255)
cTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAAt > 2:4635371/1‑76 (MQ=255)
cTGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAAt > 1:3744409/1‑76 (MQ=255)
tGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATa > 2:4788580/1‑76 (MQ=255)
tGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATa > 1:1573432/1‑76 (MQ=255)
tGATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATa < 1:777985/76‑1 (MQ=255)
gATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATac > 1:624395/1‑76 (MQ=255)
gATCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGACCCAATac > 1:6895011/1‑76 (MQ=255)
aTCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATaca < 1:3739052/76‑1 (MQ=255)
aTCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATaca < 1:573065/76‑1 (MQ=255)
aTCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATaca < 1:1772623/76‑1 (MQ=255)
aTCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATaca < 1:6886139/76‑1 (MQ=255)
tCAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATacaa < 1:192531/76‑1 (MQ=255)
cAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATacaac < 2:1035005/76‑1 (MQ=255)
cAGTTCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATacaac < 1:1059633/76‑1 (MQ=255)
|
ATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAAC > REL606/3326493‑3326638
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
