Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A59 F1 I1 R1
|
21 |
55.8 |
3542164 |
96.7% |
3425272 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,968,634 |
G→A |
G305G (GGC→GGT) |
speB ← |
agmatinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,968,634 | 0 | G | A | 98.2%
| 193.2
/ NA
| 56 | G305G (GGC→GGT) | speB | agmatinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (25/29); minor base C (0/1); total (25/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
AAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTCCGA > REL606/2968565‑2968705
|
aaGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCttt > 2:1233849/1‑76 (MQ=255)
aGGCGTGCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCtttt < 2:1644117/76‑1 (MQ=255)
ggCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCttttt < 2:1010868/76‑1 (MQ=255)
gTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGTGCATCGCATCTGGTGCTTACTCACCCTTTTTcgc > 2:572326/1‑76 (MQ=255)
tCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTcgccg > 1:546175/1‑76 (MQ=255)
cACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTcgccgc > 2:1702854/1‑76 (MQ=255)
aCGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCgccgcc < 2:822713/76‑1 (MQ=255)
cgccgcATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCt < 1:1171858/76‑1 (MQ=255)
cgccgcATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCt < 2:1640122/76‑1 (MQ=255)
gccgcATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTg < 2:408589/76‑1 (MQ=255)
aTCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGTTTGAatat > 2:157225/1‑76 (MQ=255)
aTCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGTCTGAatat < 2:1485908/76‑1 (MQ=255)
aTCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAatat < 2:41356/76‑1 (MQ=255)
aTCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAatat > 1:1756162/1‑76 (MQ=255)
ccGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATAc < 1:1303535/76‑1 (MQ=255)
ccGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATAc > 1:496894/1‑76 (MQ=255)
aTTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCAt < 1:1742292/76‑1 (MQ=255)
aTTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCAt > 2:129011/1‑76 (MQ=255)
ttAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCAtt < 1:1747140/76‑1 (MQ=255)
tAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCAttt < 2:1562220/76‑1 (MQ=255)
aaTGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTc < 1:1392483/76‑1 (MQ=255)
aaTGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTc > 2:573458/1‑76 (MQ=255)
aTGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTcc < 2:530083/76‑1 (MQ=255)
aTGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTcc > 1:488186/1‑76 (MQ=255)
tGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTcca < 2:1603461/76‑1 (MQ=255)
tGGCACATTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTcca < 1:1341271/76‑1 (MQ=255)
ggCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTccag < 2:1099846/76‑1 (MQ=255)
cGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgcc < 1:67747/76‑1 (MQ=255)
ttttACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccag > 2:726917/1‑76 (MQ=255)
tttACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagc < 2:238770/76‑1 (MQ=255)
ttACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagcg > 1:1363405/1‑76 (MQ=255)
ttACCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagcg > 1:23858/1‑76 (MQ=255)
aCCCGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGtt > 1:1400440/1‑76 (MQ=255)
cGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGcc < 1:759983/76‑1 (MQ=255)
cGTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGcc < 1:1478223/76‑1 (MQ=255)
gTGCGCATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCg > 1:1470165/1‑76 (MQ=255)
gcgcATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCt < 2:1514513/76‑1 (MQ=255)
gcgcATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCt < 1:1514289/76‑1 (MQ=255)
gcgcATCGCATCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCt < 2:1164919/76‑1 (MQ=255)
cgcatcgcatCTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTg > 2:1559393/1‑76 (MQ=255)
catcgcatcTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGcc > 2:70380/1‑76 (MQ=255)
tcgcatcTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCag < 2:1576137/76‑1 (MQ=255)
cgcatcTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCaga < 1:510073/76‑1 (MQ=255)
gcgtcTGGTGCTTACTCCCCCTGTGTCGCCGCCTGAATCTACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCagag < 2:145901/73‑1 (MQ=255)
catcTGGTGCTTACTCTCCGTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGc < 2:82250/76‑1 (MQ=255)
atcTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCa > 2:614983/1‑76 (MQ=255)
atcTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCa > 1:1066815/1‑76 (MQ=255)
cTGGTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCCGt > 2:359068/1‑76 (MQ=255)
ggTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGa < 1:1265994/76‑1 (MQ=255)
gTGCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGAt > 2:1667180/1‑76 (MQ=255)
gCTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGAttt < 1:1086427/76‑1 (MQ=255)
cTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTc < 1:1163450/76‑1 (MQ=255)
cTTACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTc > 1:1527600/1‑76 (MQ=255)
tACTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTCCg > 2:10169/1‑76 (MQ=255)
ncTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTCCGa > 1:558539/2‑76 (MQ=255)
aCTCACCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTCCGa > 1:1187996/1‑76 (MQ=255)
|
AAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCCGCTGCCAGAGCAGTGATTTCCGA > REL606/2968565‑2968705
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
