Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
19 |
34.1 |
2156969 |
96.4% |
2079318 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,339,322 |
T→C |
V54A (GTA→GCA) |
fis → |
DNA‑binding protein Fis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,339,322 | 0 | T | C | 97.8%
| 178.3
/ ‑4.1
| 46 | V54A (GTA→GCA) | fis | DNA‑binding protein Fis |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (24/21); total (24/22) |
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 |
TCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGTACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGTGCT > REL606/3339249‑3339392
|
tCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCAc > 2:1015258/1‑76 (MQ=255)
cGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACt < 2:333894/76‑1 (MQ=255)
ggTTAAACAGGCACCGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTg < 2:675334/76‑1 (MQ=255)
ttAAACAGGCACTGAAGAACTATTTTTCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGc > 1:455632/1‑76 (MQ=255)
tAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCt > 1:782563/1‑76 (MQ=255)
aaCAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCActgactga > 1:220022/1‑76 (MQ=255)
aGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGaaat > 1:342907/1‑74 (MQ=255)
gCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAg < 1:1025532/76‑1 (MQ=255)
naCNGNAGTACGATNTTTCTGACGTGAATGGTNAGGATGTGGATGACCTCTATGAGCTGACGCTGGCTGAAGTAGa < 1:915223/75‑1 (MQ=255)
ctcTGAAGAACTATTTTGCTCAACTGAATCGTCAGGATGTCAATGACCGCGATGATCTGGCACTGGCTGAAGTAGa < 1:117866/74‑1 (MQ=255)
aCTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGTACTGGTTGAAGTAGaa < 1:521122/76‑1 (MQ=255)
cTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAAc < 2:606926/76‑1 (MQ=255)
tGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACa < 1:355377/76‑1 (MQ=255)
tGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACa < 1:342926/76‑1 (MQ=255)
tGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACa < 2:820253/76‑1 (MQ=255)
aagaaCTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGc > 2:612290/1‑76 (MQ=255)
aCTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCt < 2:650106/76‑1 (MQ=255)
cTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTg < 2:790222/76‑1 (MQ=255)
tATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGt > 1:255582/1‑76 (MQ=255)
tttGCTCAACTGAATGGTCAGGATGTGAATGCCCTCTATGAGCT‑GCACTGGCTGAAGTAGAACAGCCCCTGTTGGa < 2:396897/76‑1 (MQ=255)
tttGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGAAGAACAGCCCCTGTTgg > 2:148495/1‑76 (MQ=255)
tttGCTCAACTGAATGGTCAGGATGTGAATGACATCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCACTGTTgg < 1:713516/76‑1 (MQ=255)
tttGCTCAACTGAATGGTCAGGATGTCAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTgg > 1:956147/1‑76 (MQ=255)
cTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACAt < 2:823244/76‑1 (MQ=255)
cTCAACTGAAGTGTCAGGCTGTGAATGACCTCTAGGGGCGGGCACTGGCTGAACGGGAACAGGCCCAGTTGGACAt < 1:106703/76‑1 (MQ=255)
tCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATg > 2:634537/1‑76 (MQ=255)
cTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGa > 2:667318/1‑76 (MQ=255)
cTGAATGGTCAGGATGTGAATGACATCTATGAGCTGGCACTGGCTGACGTAGAACAGCCCCTGTTGTAGATGGGGa > 2:667314/1‑76 (MQ=255)
cTGAATGGTCAGGATGTGAATCACGCATATGCGCTGGCACCGACTGAAGTAGTACAGGCCCTGCTCGACATGGTgc > 1:992797/1‑75 (MQ=255)
tGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGAt > 2:625250/1‑76 (MQ=255)
tGAATGGCCAGGACGTGGATGACCTCTCTGAGCTGGCACTGGCCGAAGTAGAACAGCCCCCGTTGGACATGGTGAt > 1:965869/1‑76 (MQ=255)
ggTCAGGATGTGAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAAt > 2:510313/1‑76 (MQ=255)
gtgAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTg < 2:45492/76‑1 (MQ=255)
tgAATGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTgg > 2:546771/1‑76 (MQ=255)
aaTGACCTCTATGAGCTGGCACTGGCTAAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTa < 2:450576/76‑1 (MQ=255)
aTGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTaa < 2:380459/76‑1 (MQ=255)
aTGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTaa < 1:718948/76‑1 (MQ=255)
tGACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAAc < 2:756435/76‑1 (MQ=255)
gACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAAcc < 2:313567/76‑1 (MQ=255)
gACCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAAcc > 1:308212/1‑76 (MQ=255)
aCCTCTATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGATAACCa > 1:307640/1‑76 (MQ=255)
tctATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGAAACCAGAc > 1:260347/1‑76 (MQ=255)
tATGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGAccc > 1:15655/1‑76 (MQ=255)
tGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCgc > 2:826589/1‑75 (MQ=255)
tGAGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGt > 2:826600/1‑76 (MQ=255)
aGCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTAGTAACCAGACCCGtgc > 2:442030/1‑76 (MQ=255)
gCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGtgct < 2:554188/76‑1 (MQ=255)
gCTGGCACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGtgct > 1:878811/1‑76 (MQ=255)
|
TCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGCTGGTACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGTGCT > REL606/3339249‑3339392
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
