mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 475,202 | C→T | G35E (GGA→GAA) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| JC JC | 968,327 | IS150 (–) +2 bp :: +AT | intergenic (‑154/+37) | pflA ← / ← pflB | pyruvate formate lyase activating enzyme 1/pyruvate formate lyase I |
| JC JC | 1,305,487 | IS150 (–) +3 bp | coding (751‑753/1461 nt) | cls ← | cardiolipin synthetase |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| RA | 3,145,813 | C→T | intergenic (+144/‑51) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| RA | 3,249,887 | C→T | M454I (ATG→ATA) | infB ← | translation initiation factor IF‑2 |
| RA | 3,326,561 | A→T | I69N (ATC→AAC) | mreD ← | cell wall structural complex MreBCD transmembrane component MreD |
| RA | 3,339,322 | T→C | V54A (GTA→GCA) | fis → | DNA‑binding protein Fis |
| RA | 3,497,971 | G→A | A22V (GCT→GTT) | glgP ← | glycogen phosphorylase |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| RA | 3,928,928 | C→T | R238C (CGC→TGC) | rho → | transcription termination factor Rho |
| RA | 3,948,122 | C→T | intergenic (‑316/+370) | aslA ← / ← hemY | acrylsulfatase‑like enzyme/predicted protoheme IX synthesis protein |
| RA | 4,162,845 | G→A | G664S (GGT→AGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| JC JC | 4,201,922 | IS150 (+) +3 bp | coding (636‑638/825 nt) | iclR ← | DNA‑binding transcriptional repressor |
| RA | 4,519,066 | T→G | N192H (AAT→CAT) | yjhA ← | N‑acetylnuraminic acid outer membrane channel protein |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | REL606 | 546993–547700 | 548611 | 912–1619 | 9 [8] | [8] 9 | [insB‑6]–nmpC | [insB‑6],insA‑6,nmpC |
| * | * | ÷ | REL606 | 548615 | 593369 | 44755 | 9 [8] | [8] 9 | ybcR–[fepA] | ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
| * | * | ÷ | REL606 | 593575 | 619823–619116 | 25542–26249 | 9 [8] | [8] 9 | [fepA]–[insA‑8] | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8] |
| * | * | ÷ | REL606 | 3895000 | 3901930 | 6931 | 9 [0] | [0] 30 | rbsD–[yieO] | rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 3652533 | NA (NA) | 29 (0.880) | 24/146 | 0.4 | 100% | noncoding (1443/1443 nt) | IS150 | repeat region |
| ? | REL606 | 3901931 = | 0 (0.000) | coding (489/1428 nt) | yieO | predicted multidrug or homocysteine efflux system | |||||