Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A119 F1 I1 R1
|
20 |
247.5 |
15984500 |
92.7% |
14817631 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,015,124 |
G→A |
G182D (GGC→GAC) |
ECB_02816 → |
KpsD protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,015,124 | 0 | G | A | 99.0%
| 1200.5
/ 1.2
| 290 | G182D (GGC→GAC) | ECB_02816 | KpsD protein |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (131/156); total (132/158) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01 |
Only 100 of 291 total aligned reads displayed.
TACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTA > REL606/3015051‑3015196
|
tACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACg < 1:2806644/76‑1 (MQ=255)
tACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACg > 1:4306794/1‑76 (MQ=255)
aCGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACgg > 1:1036537/1‑76 (MQ=255)
gCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGgtg > 2:3723898/1‑76 (MQ=255)
cctcctTATTCCGGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGgtgt < 1:3411755/76‑1 (MQ=255)
cctcctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGgtgt > 1:6729964/1‑76 (MQ=255)
ctcctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGtgtg < 1:3554069/76‑1 (MQ=255)
tcctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGa < 1:7771800/76‑1 (MQ=255)
cctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGAc > 2:7672115/1‑76 (MQ=255)
ttattaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGt > 2:2181138/1‑76 (MQ=255)
tattaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTc > 2:6529247/1‑76 (MQ=255)
tattaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTc > 2:5015754/1‑76 (MQ=255)
tattaCAGGCCCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTc < 1:7628796/76‑1 (MQ=255)
attaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCt > 2:7584081/1‑76 (MQ=255)
attaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCt > 2:201158/1‑76 (MQ=255)
ttaCGGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTg < 2:6355120/76‑1 (MQ=255)
ttaCATGCGCAGCCAGGAAAAGTGTACGAGACCGGATTTGTGCGGCATCCTGGTCTGTATGACGGAGTGACGTCTg > 2:3217483/1‑76 (MQ=255)
ttaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTg < 2:4749286/76‑1 (MQ=255)
cAGCCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGAtt < 1:5320342/76‑1 (MQ=255)
aGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTc < 1:2008224/76‑1 (MQ=255)
gcAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTa > 1:1629546/1‑76 (MQ=255)
ccAGCCAGTAAAAGTGTACGTGTCCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTa < 1:3123917/75‑1 (MQ=255)
cAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTAc > 1:4470158/1‑76 (MQ=255)
gCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTc > 1:5946338/1‑76 (MQ=255)
ccAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCa > 1:6863611/1‑76 (MQ=255)
ccAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCa < 1:7558209/76‑1 (MQ=255)
ccAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCa < 1:1141918/76‑1 (MQ=255)
cAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCaa < 1:1564822/76‑1 (MQ=255)
aGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAAt > 2:6239482/1‑76 (MQ=255)
gTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAAtt < 2:5215605/76‑1 (MQ=255)
tAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTa > 2:3462080/1‑76 (MQ=255)
aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt > 1:6342260/1‑76 (MQ=255)
aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt > 1:4584962/1‑76 (MQ=255)
aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt > 2:2774627/1‑76 (MQ=255)
aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt < 2:4954913/76‑1 (MQ=255)
tgtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGAt < 2:2631896/76‑1 (MQ=255)
tgtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGAt < 2:4037615/76‑1 (MQ=255)
tgtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGAt > 2:956802/1‑76 (MQ=255)
gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc > 2:2439346/1‑76 (MQ=255)
gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc > 1:2594442/1‑76 (MQ=255)
gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc > 2:7465476/1‑76 (MQ=255)
gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc < 2:7008335/76‑1 (MQ=255)
gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc > 2:6888150/1‑76 (MQ=255)
tACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCa < 1:6358134/76‑1 (MQ=255)
tACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCa > 1:2216842/1‑76 (MQ=255)
tACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCa < 1:574883/76‑1 (MQ=255)
aCGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCATTACTCAATTATCTGATCaa < 1:5011216/76‑1 (MQ=255)
cGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAg < 1:7181447/76‑1 (MQ=255)
cGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAg < 2:835667/76‑1 (MQ=255)
tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc > 1:2972611/1‑76 (MQ=255)
tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc > 2:3844604/1‑76 (MQ=255)
tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc > 2:3126139/1‑76 (MQ=255)
tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc < 1:4055618/76‑1 (MQ=255)
gACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCt > 1:5672150/1‑76 (MQ=255)
gACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCt < 2:1284331/76‑1 (MQ=255)
aCCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTg < 2:7879716/76‑1 (MQ=255)
aCCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTg > 2:4366367/1‑76 (MQ=255)
ccGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTgg > 1:1420573/1‑76 (MQ=255)
cGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggg > 2:795077/1‑75 (MQ=255)
cGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggg > 2:4624546/1‑75 (MQ=255)
cGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggc < 1:232742/76‑1 (MQ=255)
tttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggcgggg > 1:5788071/1‑74 (MQ=255)
tttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGgcggcg > 1:196322/1‑76 (MQ=255)
ttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTgggggggg > 2:5497927/1‑73 (MQ=255)
ttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGt > 2:7502137/1‑76 (MQ=255)
gtgCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTg < 2:1385590/76‑1 (MQ=255)
tgCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGa < 2:6041029/76‑1 (MQ=255)
gCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGAt > 1:5592201/1‑76 (MQ=255)
gTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATcc > 1:3944908/1‑76 (MQ=255)
tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGGGGGGTTGATTCa > 1:6627696/1‑76 (MQ=255)
tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCa < 1:5189043/76‑1 (MQ=255)
tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCa < 1:370983/76‑1 (MQ=255)
tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGGTGATTCa > 2:3062773/1‑76 (MQ=255)
aaTCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATcccg > 1:6153058/1‑74 (MQ=255)
aaTCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCag > 1:4834256/1‑76 (MQ=255)
aaTCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCag < 2:1162735/76‑1 (MQ=255)
cTGGTCTGTATGACGTTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGGGGTGGTCCagaggg > 1:2079765/1‑74 (MQ=255)
cTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGGGGGGGTGATCTAGAgcg > 1:3254152/1‑76 (MQ=255)
tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGGGGCGTTGATCCCGAgggc > 2:6625924/1‑76 (MQ=255)
tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTTATCCCGAgcgg > 1:2780235/1‑75 (MQ=255)
tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAgcgc < 2:39206/76‑1 (MQ=255)
tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAgcgc < 1:3818527/76‑1 (MQ=255)
gTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCgg < 2:3685807/76‑1 (MQ=255)
gTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCgg < 2:3608814/76‑1 (MQ=255)
tCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGa < 2:5058733/76‑1 (MQ=255)
tCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGa > 2:7708095/1‑76 (MQ=255)
tCTGTATGACGGTGTGAAGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCCGa > 2:2910800/1‑76 (MQ=255)
cTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGGTCCAGAGCGCggca > 2:5514590/1‑74 (MQ=255)
cTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGGGCggga > 1:894746/1‑74 (MQ=255)
cTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGaa < 2:2496762/76‑1 (MQ=255)
tGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAg < 1:3786498/76‑1 (MQ=255)
tGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAg < 2:3386396/76‑1 (MQ=255)
gTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGt < 2:7241898/76‑1 (MQ=255)
tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGGTCCCGAGCGCGGAAGtt > 1:6229159/1‑76 (MQ=255)
tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt < 1:7269395/76‑1 (MQ=255)
tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt < 1:3014762/76‑1 (MQ=255)
tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt < 2:2354553/76‑1 (MQ=255)
tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt < 1:6418956/76‑1 (MQ=255)
aTGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTa < 2:5918981/76‑1 (MQ=255)
aTGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTa < 2:2843655/76‑1 (MQ=255)
|
TACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTA > REL606/3015051‑3015196
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
