Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A54 F1 I1 R1
|
16 |
53.7 |
3449390 |
96.4% |
3325211 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,479,649 |
C→T |
noncoding (17/85 nt) |
leuX → |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,479,649 | 0 | C | T | 98.5%
| 261.3
/ ‑3.6
| 67 | noncoding (17/85 nt) | leuX | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (38/28); total (39/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01 |
TGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAA > REL606/4479577‑4479720
|
tGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGt > 1:1360173/1‑76 (MQ=255)
tGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGt > 1:258592/1‑76 (MQ=255)
cATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAg < 1:366973/76‑1 (MQ=255)
aTGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGa > 2:1321004/1‑76 (MQ=255)
tggtggCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGAc > 2:868028/1‑76 (MQ=255)
tggtggCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGAc < 1:1515623/76‑1 (MQ=255)
gtggCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGc > 1:710909/1‑76 (MQ=255)
cGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGtt > 2:1519286/1‑76 (MQ=255)
cGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGtt > 1:790427/1‑76 (MQ=255)
tGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGa > 2:408839/1‑76 (MQ=255)
cGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGAtt > 2:780427/1‑76 (MQ=255)
cAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCaa < 2:1384081/76‑1 (MQ=255)
aGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCaaa > 2:1380885/1‑76 (MQ=255)
ggTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCaaaa > 1:387838/1‑76 (MQ=255)
ataATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCa > 2:551442/1‑76 (MQ=255)
ataATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCa < 1:1608618/76‑1 (MQ=255)
taATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCaa > 2:457249/1‑76 (MQ=255)
aaTCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAAc > 2:1111857/1‑76 (MQ=255)
tCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCg > 1:1033078/1‑76 (MQ=255)
ccACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGt < 2:1443642/76‑1 (MQ=255)
acaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAg < 1:385519/76‑1 (MQ=255)
caACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGa < 2:870367/76‑1 (MQ=255)
aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGaa < 1:979748/76‑1 (MQ=255)
aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGaa < 1:1555176/76‑1 (MQ=255)
aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGaa > 2:815720/1‑76 (MQ=255)
aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGaa > 2:1243964/1‑76 (MQ=255)
gTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATa < 2:282707/76‑1 (MQ=255)
gTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATa < 1:294139/76‑1 (MQ=255)
ttCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGt > 1:183759/1‑76 (MQ=255)
tCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTg < 2:1632699/76‑1 (MQ=255)
tCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTg > 2:1532091/1‑76 (MQ=255)
tCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTg < 2:998868/76‑1 (MQ=255)
cATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCgg < 2:1145608/76‑1 (MQ=255)
cATACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCgg > 1:1674571/1‑76 (MQ=255)
tACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGtt < 1:130333/76‑1 (MQ=255)
tACCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGtt > 1:95680/1‑76 (MQ=255)
aCCTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTc > 1:1574990/1‑76 (MQ=255)
ccTCTTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCg < 1:78507/76‑1 (MQ=255)
ctctTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGa > 1:974387/1‑76 (MQ=255)
tctTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAg > 2:1357732/1‑76 (MQ=255)
ctTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGt > 2:1426034/1‑76 (MQ=255)
ctTCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGt > 2:1426048/1‑76 (MQ=255)
tCAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTcc < 2:90122/76‑1 (MQ=255)
cAGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCg < 2:164364/76‑1 (MQ=255)
aGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgg > 1:1348652/1‑76 (MQ=255)
aGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgg > 1:1321857/1‑76 (MQ=255)
aGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgg < 2:1325836/76‑1 (MQ=255)
aGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgg > 1:362368/1‑76 (MQ=255)
aGTGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgg < 2:419448/76‑1 (MQ=255)
tGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgggc > 1:786660/1‑74 (MQ=255)
tGCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGcc > 1:771209/1‑76 (MQ=255)
gCCGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCt < 2:1434205/76‑1 (MQ=255)
ccGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCtt < 2:121510/76‑1 (MQ=255)
ccGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCtt > 2:1023276/1‑76 (MQ=255)
ccGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCtt < 2:609026/76‑1 (MQ=255)
ccGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCtt > 2:1416574/1‑76 (MQ=255)
cGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGGCTTc > 2:899570/1‑76 (MQ=255)
cGAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTc > 2:892697/1‑76 (MQ=255)
gAAGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCg > 2:314494/1‑76 (MQ=255)
aaGTGGCGAAGTTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCgg < 1:687390/76‑1 (MQ=255)
aaGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCgg < 2:492950/76‑1 (MQ=255)
aGTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGc < 2:1348651/76‑1 (MQ=255)
gTGGCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCa < 2:672397/76‑1 (MQ=255)
tGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCCTCGGCAc > 2:587759/1‑76 (MQ=255)
ggCGAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCAcc > 1:1182396/1‑76 (MQ=255)
gAAATTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCaaa < 1:777712/76‑1 (MQ=255)
aaaTTGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCaaaa > 2:697730/1‑76 (MQ=255)
|
TGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAA > REL606/4479577‑4479720
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
