breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 474,186 T→A I374F (ATC→TTC)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
JC JC 1,305,418 IS150 (–) +3 bp coding (820‑822/1461 nt) cls ← cardiolipin synthetase
RA 1,329,281 C→G intergenic (‑241/‑139) yciN ← / → topA hypothetical protein/DNA topoisomerase I
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,360,241 G→A V635I (GTT→ATT)  pta → phosphate acetyltransferase
RA 2,897,276 C→T Q21* (CAA→TAA)  yqeA → predicted amino acid kinase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,928,259 A→T I15F (ATC→TTC)  rho → transcription termination factor Rho
RA 4,103,979 T→A intergenic (‑40/+116) cytR ← / ← priA DNA‑binding transcriptional dual regulator/primosome assembly protein PriA
RA 4,202,096 G→A P155L (CCG→CTG)  iclR ← DNA‑binding transcriptional repressor
RA 4,479,649 C→T noncoding (17/85 nt) leuX → tRNA‑Leu
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 2032380–2032460 2041713 9254–9334 16 [14] [15] 16 [manB]–[wzx] [manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,[wzx]
* * ÷ REL606 2041742 2055702–2055576 13835–13961 16 [15] [15] 16 [wzx]–[cpsG] [wzx],rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3651091 =NA (NA)57 (1.100) 43/144 0.2 NA noncoding (1/1443 nt) IS150 repeat region
?REL606 3907731 = NA (NA)noncoding (2230/2906 nt) rrlC 23S ribosomal RNA
* ? REL606 = 3894996NA (NA)75 (1.410) 55/148 0.0 NA noncoding (1443/1443 nt) IS150 repeat region
?REL606 = 3907733 NA (NA)noncoding (2232/2906 nt) rrlC 23S ribosomal RNA