Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A143 F1 I1 R1
|
16 |
39.8 |
2536279 |
96.2% |
2439900 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,271,597 |
G→A |
P45S (CCG→TCG) |
murA ← |
UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,271,597 | 0 | G | A | 100.0%
| 115.1
/ NA
| 31 | P45S (CCG→TCG) | murA | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/18); total (13/18) |
GAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAG > REL606/3271524‑3271668
|
gAACCATTAAGTGCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGANGTCTTTCAGTTTCgaga < 1:153240/76‑1 (MQ=255)
aaCCATTTCGATCTACTTGCGCACACATCTGGCCTAGCTNNTTCATTGNTGTATCGACGTCTTTGAGTTTCGAGAc < 1:1248236/76‑1 (MQ=255)
ccATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGt > 2:64417/1‑76 (MQ=255)
aTTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCANTGATGTATCGACGTCTTTNAGNTTCGAGACGTTc > 1:1039100/1‑76 (MQ=255)
cGTTCTTCTATCGCACCGTGCTGGCTTAGCAGCTTCATTGATGTATCGAGGTCTTTCAGTANCGNGACGTTCTGGa > 2:195350/1‑76 (MQ=255)
tttCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTAcc > 2:799721/1‑76 (MQ=255)
tCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCgg > 2:407342/1‑76 (MQ=255)
tCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCgg > 2:796678/1‑76 (MQ=255)
gCACCCAGCTGGCTTAGCAGCTTCACTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGtt > 2:905847/1‑76 (MQ=255)
ccccAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGttct < 2:112504/75‑1 (MQ=255)
aCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGttct > 2:888447/1‑76 (MQ=255)
cAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTcc > 1:556895/1‑76 (MQ=255)
aGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCg > 1:689340/1‑76 (MQ=255)
ggCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGTATCTCTACCGGTTCTTCCGCCAg < 1:510910/76‑1 (MQ=255)
gCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGt < 2:735323/76‑1 (MQ=255)
cTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTa < 1:986332/76‑1 (MQ=255)
ttAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTag < 2:960961/76‑1 (MQ=255)
tAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTaga > 2:30039/1‑76 (MQ=255)
cagcTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCg < 1:734507/76‑1 (MQ=255)
gcTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGc > 1:956398/1‑76 (MQ=255)
cTTCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCa < 2:1082089/76‑1 (MQ=255)
ttCATTGATGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCaa < 2:829088/76‑1 (MQ=255)
aTGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGAt < 1:744030/76‑1 (MQ=255)
tGTATCGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATa < 1:389881/76‑1 (MQ=255)
cGACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCag < 2:761473/76‑1 (MQ=255)
gACGTCTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCaga < 1:464996/76‑1 (MQ=255)
aCGTCTTTCAGTTTCGAGACGTACTGGNGCNCTNCCAGTTCNTCCGCCCGTAGAGCGGCAAAAAGGATAGGCagag < 1:1103296/76‑1 (MQ=255)
cTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGagcagc < 2:1008864/76‑1 (MQ=255)
cTTTCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGagcagc > 2:487117/1‑76 (MQ=255)
ttCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCAt < 2:239659/76‑1 (MQ=255)
ttCAGTTTCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCAt > 1:924913/1‑76 (MQ=255)
tCAGTTTCGAGACGTGCTGGATCTCTGCCGGTTCGTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCAtt < 2:1128022/76‑1 (MQ=255)
tttCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTa < 1:888448/76‑1 (MQ=255)
ttCGAGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAg < 2:847587/76‑1 (MQ=255)
|
GAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGAGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAG > REL606/3271524‑3271668
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
