Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A64 F1 I1 R1
|
17 |
58.5 |
3743480 |
96.8% |
3623688 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,279,263 |
G→A |
G29R (GGG→AGG) |
yhbG → |
predicted transporter subunit: ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,279,263 | 0 | G | A | 96.3%
| 198.4
/ ‑2.1
| 54 | G29R (GGG→AGG) | yhbG | predicted transporter subunit: ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (26/26); total (27/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
ACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTG > REL606/3279191‑3279335
|
aCTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCaggg < 2:329671/76‑1 (MQ=255)
cTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGa > 1:527285/1‑76 (MQ=255)
cAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAt < 1:508424/76‑1 (MQ=255)
aaaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtc > 2:84394/1‑76 (MQ=255)
aaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcg > 1:129351/1‑76 (MQ=255)
aaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcg > 2:1406017/1‑76 (MQ=255)
aaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcg > 2:1353634/1‑76 (MQ=255)
gAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAAtcgtc < 1:1339222/76‑1 (MQ=255)
gAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcgtc > 1:1608381/1‑76 (MQ=255)
aaCCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcgtcg > 1:873144/1‑76 (MQ=255)
aCCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCgg < 1:1516884/76‑1 (MQ=255)
ccTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGt > 1:1444663/1‑76 (MQ=255)
ttGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTct < 1:1795622/76‑1 (MQ=255)
tGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTctg < 2:194957/76‑1 (MQ=255)
gCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTctgc > 2:732130/1‑76 (MQ=255)
aaaaGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTctgctg > 1:1219352/1‑76 (MQ=255)
aaGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTggg < 2:543586/76‑1 (MQ=255)
aGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTgggg > 1:352807/1‑76 (MQ=255)
ccTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGcc > 2:951713/1‑76 (MQ=255)
ataAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCaaa > 1:309095/1‑76 (MQ=255)
aaaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACg < 2:118352/76‑1 (MQ=255)
aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:404940/1‑76 (MQ=255)
aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:1542700/1‑76 (MQ=255)
aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:1510191/1‑76 (MQ=255)
aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 1:590736/1‑76 (MQ=255)
aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:1002953/1‑76 (MQ=255)
tCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGt < 1:1028032/76‑1 (MQ=255)
tCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGt < 2:1138650/76‑1 (MQ=255)
tCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGt < 1:1811610/76‑1 (MQ=255)
gcgTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTaa < 1:1401017/76‑1 (MQ=255)
cgTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAg < 1:162147/76‑1 (MQ=255)
cgTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAg > 2:138050/1‑76 (MQ=255)
gTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGTCGGTAAGa < 1:1338779/76‑1 (MQ=255)
gTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGa < 1:152949/76‑1 (MQ=255)
tGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGac < 1:1308184/76‑1 (MQ=255)
gTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGacca > 2:1842463/1‑76 (MQ=255)
tAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAATCGTCGGTCTGTTGGGGCCAAACGGTGCCGGTAAGaccac > 1:1776491/1‑76 (MQ=255)
tAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGATGCCGGTAAGaccac < 1:1412532/76‑1 (MQ=255)
agaagaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGaccacc > 1:1513349/1‑76 (MQ=255)
aagaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACcaccac > 2:1264481/1‑76 (MQ=255)
aagaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACcaccac > 2:1214777/1‑76 (MQ=255)
gaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACtt > 1:437162/1‑76 (MQ=255)
gaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACtt > 1:401496/1‑76 (MQ=255)
gaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACtt < 1:368167/76‑1 (MQ=255)
aCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACttt < 2:810207/76‑1 (MQ=255)
cGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTc < 2:1440654/76‑1 (MQ=255)
gTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCt < 1:951713/76‑1 (MQ=255)
tGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGt < 1:1115850/76‑1 (MQ=255)
tGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGt > 2:1460441/1‑76 (MQ=255)
ccGTCAACTCCAGGAAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGt < 1:93429/76‑1 (MQ=255)
cGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTa > 1:1267696/1‑76 (MQ=255)
gTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAg < 2:691058/76‑1 (MQ=255)
cAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGc < 2:1771357/76‑1 (MQ=255)
cTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCAtt < 2:869788/76‑1 (MQ=255)
tCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATtg < 1:604390/76‑1 (MQ=255)
tCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATtg < 1:594057/76‑1 (MQ=255)
|
ACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTG > REL606/3279191‑3279335
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
