breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 124,866 | A→G | intergenic (‑511/‑30) | aroP ← / → pdhR | aromatic amino acid transporter/transcriptional regulator of pyruvate dehydrogenase complex |
JC | 474,391 | (GTCGCCAGC)1→2 | coding (915/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 649,991 | C→T | A271T (GCA→ACA) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,165,450 | C→A | R131L (CGC→CTC) | yehU ← | predicted sensory kinase in two‑component system with YehT |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
MC JC | 2,490,667 | Δ16 bp | coding (485‑500/837 nt) | eutJ ← | predicted chaperonin, ethanolamine utilization protein |
RA | 2,740,354 | G→A | G326G (GGC→GGT) | hycE ← | hydrogenase 3, large subunit |
JC | 3,014,068 | (AAC)3→2 | coding (649‑651/1137 nt) | ECB_02815 → | Capsule polysaccharide export inner‑membrane protein kpsE |
RA | 3,145,987 | G→A | E42K (GAA→AAA) | rpoD → | RNA polymerase sigma factor |
RA | 3,279,263 | G→A | G29R (GGG→AGG) | yhbG → | predicted transporter subunit: ATP‑binding component of ABC superfamily |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 4,163,749 | A→C | Q965P (CAG→CCG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 3697036–3697074 | 3698227–3697080 | 7–1192 | 20 [19] | [18] 21 | rhsA | rhsA element core protein RshA |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2299029 = | 6 (0.100) | 59 (1.010) | 46/150 | 0.2 | 90.8% | coding (54/1359 nt) | glpT | sn‑glycerol‑3‑phosphate transporter |
? | REL606 | = 2299035 | NA (NA) | coding (48/1359 nt) | glpT | sn‑glycerol‑3‑phosphate transporter |