breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,866 A→G intergenic (‑511/‑30) aroP ← / → pdhR aromatic amino acid transporter/transcriptional regulator of pyruvate dehydrogenase complex
JC 474,391 (GTCGCCAGC)1→2 coding (915/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 649,991 C→T A271T (GCA→ACA)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,165,450 C→A R131L (CGC→CTC)  yehU ← predicted sensory kinase in two‑component system with YehT
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
MC JC 2,490,667 Δ16 bp coding (485‑500/837 nt) eutJ ← predicted chaperonin, ethanolamine utilization protein
RA 2,740,354 G→A G326G (GGC→GGT hycE ← hydrogenase 3, large subunit
JC 3,014,068 (AAC)3→2 coding (649‑651/1137 nt) ECB_02815 → Capsule polysaccharide export inner‑membrane protein kpsE
RA 3,145,987 G→A E42K (GAA→AAA)  rpoD → RNA polymerase sigma factor
RA 3,279,263 G→A G29R (GGG→AGG)  yhbG → predicted transporter subunit: ATP‑binding component of ABC superfamily
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,163,749 A→C Q965P (CAG→CCG)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3697036–3697074 3698227–3697080 7–1192 20 [19] [18] 21 rhsA rhsA element core protein RshA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2299029 =6 (0.100)59 (1.010) 46/150 0.2 90.8% coding (54/1359 nt) glpT sn‑glycerol‑3‑phosphate transporter
?REL606 = 2299035 NA (NA)coding (48/1359 nt) glpT sn‑glycerol‑3‑phosphate transporter