Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A70 F1 I1 R1
|
18 |
48.6 |
3152226 |
95.8% |
3019832 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,896 |
T→C |
L1014P (CTG→CCG) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,896 | 0 | T | C | 100.0%
| 137.8
/ NA
| 34 | L1014P (CTG→CCG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/13); total (21/13) |
GACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCA > REL606/4163823‑4163968
|
gACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAgccgg > 2:290599/1‑76 (MQ=255)
aaaCTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAgccggctg < 2:163286/76‑1 (MQ=255)
aaCTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGa > 1:52148/1‑76 (MQ=255)
gCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAg > 1:1043415/1‑76 (MQ=255)
gCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAg > 1:1204195/1‑76 (MQ=255)
gCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAg > 2:1516042/1‑76 (MQ=255)
gCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAg > 1:284307/1‑76 (MQ=255)
ccGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGt < 2:1573017/76‑1 (MQ=255)
gcgcgATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTAt < 2:442147/76‑1 (MQ=255)
gcgcgATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTAt < 1:41236/76‑1 (MQ=255)
cgcgATCGCTGGCTGGAGCTGGGCCTGACTGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATg < 2:1126289/76‑1 (MQ=255)
cgcgATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATg < 1:1271452/76‑1 (MQ=255)
cgcgATCGCGGGCTGGAGCTGGGCCTGGCCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATg < 2:776064/76‑1 (MQ=255)
gcgATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGa < 1:619269/76‑1 (MQ=255)
ggctggAGCTGGGCCTGACCGACAAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGaa < 1:231591/76‑1 (MQ=255)
gAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACg > 2:319804/1‑76 (MQ=255)
gggCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTc > 2:1024274/1‑76 (MQ=255)
gCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCga > 1:604508/1‑76 (MQ=255)
aCCGGCGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCgggatgt < 2:170913/76‑4 (MQ=255)
ccGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAgaagaa > 2:1297029/1‑76 (MQ=255)
ccGACGAAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAgaagaa > 1:1037818/1‑76 (MQ=255)
cgacgaAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGaaa < 2:723787/76‑1 (MQ=255)
acgaAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACt < 2:1024403/76‑1 (MQ=255)
acgaAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACt > 1:749590/1‑76 (MQ=255)
gaAGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCg > 1:1322845/1‑76 (MQ=255)
aaGAGAAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGa > 1:1203828/1‑76 (MQ=255)
agaAACAAAATCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGc > 2:1042435/1‑76 (MQ=255)
aCAAAATCAGCGGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGaaa < 1:176040/76‑1 (MQ=255)
aaaaTCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAAcg > 1:865770/1‑76 (MQ=255)
aaaTCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAAcgc > 1:1338313/1‑76 (MQ=255)
aaaTCAGCTGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAAcgc > 1:822475/1‑76 (MQ=255)
tGGAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAAt > 2:176142/1‑76 (MQ=255)
gAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCa > 2:1027803/1‑76 (MQ=255)
gAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCa > 2:46661/1‑76 (MQ=255)
gAACAGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCa < 1:646045/76‑1 (MQ=255)
aGCCGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCa > 1:855573/1‑76 (MQ=255)
|
GACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCA > REL606/4163823‑4163968
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
