breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 305,821 T→G Y268D (TAT→GAT)  yahA → predicted DNA‑binding transcriptional regulator
JC 475,145 Δ13 bp coding (149‑161/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
MC JC 1,290,853 Δ92 bp coding (818‑909/1014 nt) hnr → response regulator of RpoS
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,882,907 T→G L148* (TTA→TGA)  manZ → mannose‑specific enzyme IID component of PTS
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
JC 2,362,391 +GGA coding (378/543 nt) yfcD ← predicted NUDIX hydrolase
RA 2,478,071 C→T M173I (ATG→ATA yfeY ← hypothetical protein
MC JC 2,904,110 (GTC)4→3 coding (2297‑2299/3099 nt) ygfK → predicted oxidoreductase, Fe‑S subunit
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,842,808 G→A A428V (GCG→GTG)  dnaA ← chromosomal replication initiation protein
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,928,383 A→C E56A (GAG→GCG)  rho → transcription termination factor Rho
RA 4,154,953 T→A intergenic (‑316/‑46) coaA ← / → thrU pantothenate kinase/tRNA‑Thr
RA 4,163,896 T→C L1014P (CTG→CCG)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2037848 =51 (1.040)28 (0.620) 13/140 2.4 29.5% coding (283/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 2038306 = 86 (1.890)coding (643/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 = 268075789 (1.820)20 (0.450) 13/138 2.4 24.6% coding (283/897 nt) ECB_02511 ParB family protein
?REL606 = 2681225 41 (0.910)coding (751/897 nt) ECB_02511 ParB family protein
* ? REL606 4097350 =24 (0.490)59 (1.280) 44/142 0.1 73.9% coding (223/846 nt) glpF glycerol facilitator
?REL606 = 4097374 19 (0.410)coding (199/846 nt) glpF glycerol facilitator