Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F1 I1 R1
|
18 |
56.1 |
3547650 |
96.9% |
3437672 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,984 |
T→G |
K18Q (AAG→CAG) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,984 | 0 | T | G | 100.0%
| 181.8
/ NA
| 44 | K18Q (AAG→CAG) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (19/25); total (19/25) |
AGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATT > REL606/3497911‑3498058
|
aGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGaa < 1:1066251/76‑1 (MQ=255)
aGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGaa > 1:845840/1‑76 (MQ=255)
aTTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGc > 2:1287241/1‑76 (MQ=255)
tCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCtt > 2:859026/1‑76 (MQ=255)
cATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTc > 2:13762/1‑76 (MQ=255)
aTGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCt < 1:1441827/76‑1 (MQ=255)
aTGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCt < 1:576575/76‑1 (MQ=255)
tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTa < 2:1688794/76‑1 (MQ=255)
tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTa < 2:198610/76‑1 (MQ=255)
gTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTAc > 1:204518/1‑76 (MQ=255)
tttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACg > 1:368082/1‑76 (MQ=255)
gacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGc < 2:1475805/76‑1 (MQ=255)
gacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGc > 2:1524555/1‑76 (MQ=255)
ccGGGTCCTTTCCAATCATAAACACCAGCTTGTAAGCGATAGAGTGCAGAAGGGCTTCTACGCTAAGCGTGGGCGa < 2:572836/76‑1 (MQ=255)
ggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGa < 1:1350013/76‑1 (MQ=255)
gTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGaa > 1:1608723/1‑76 (MQ=255)
ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAata < 1:948308/76‑1 (MQ=255)
cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGTGATGAatat > 2:799734/1‑76 (MQ=255)
cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAatat < 2:562362/76‑1 (MQ=255)
cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAatat < 1:1362586/76‑1 (MQ=255)
tCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTa < 1:32922/76‑1 (MQ=255)
tCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTa > 1:1229849/1‑76 (MQ=255)
cAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaaa < 1:946412/76‑1 (MQ=255)
cAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaaa < 2:1096380/76‑1 (MQ=255)
tCGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACgg < 1:468231/76‑1 (MQ=255)
cGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa > 1:1427947/1‑76 (MQ=255)
cGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa < 2:1330483/76‑1 (MQ=255)
cGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa > 2:1598818/1‑76 (MQ=255)
cGTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa > 1:291603/1‑76 (MQ=255)
gTAAACATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAg > 2:1627158/1‑76 (MQ=255)
aaaCATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa > 1:1123927/1‑76 (MQ=255)
aaaCATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa < 1:516574/76‑1 (MQ=255)
aaaCATCAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa < 1:167208/76‑1 (MQ=255)
cAGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCata < 2:1171402/76‑1 (MQ=255)
aGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat < 2:420426/76‑1 (MQ=255)
aGCTTGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat < 1:722101/76‑1 (MQ=255)
tGTAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGc > 2:805708/1‑76 (MQ=255)
tAAGCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGt < 2:219445/76‑1 (MQ=255)
gCGATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTc < 1:335802/76‑1 (MQ=255)
gATAGAGTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCt > 2:1157516/1‑76 (MQ=255)
agagTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaa > 1:987900/1‑76 (MQ=255)
agagTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaa < 2:1704095/76‑1 (MQ=255)
agTGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaaaa < 1:887419/76‑1 (MQ=255)
tGCTGAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACt > 2:37316/1‑76 (MQ=255)
ttAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTAtt > 2:1455072/1‑76 (MQ=255)
|
AGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATT > REL606/3497911‑3498058
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
