mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| JC JC | 1,305,419 | IS150 (+) +3 bp | coding (819‑821/1461 nt) | cls ← | cardiolipin synthetase |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| RA | 1,779,616 | G→A | S74S (TCC→TCT) | thrS ← | threonyl‑tRNA synthetase |
| RA | 1,995,676 | (C)8→7 | intergenic (+103/+351) | yeeN → / ← asnW | hypothetical protein/tRNA‑Asn |
| MC JC | 2,100,308 | Δ22,146 bp | ogrK–[ECB_02013] | 27 genes ogrK, yegZ, ECB_01989, ECB_01990, ECB_01991, ECB_01992, ECB_01993, ECB_01994, ECB_01995, ECB_01996, ECB_01997, ECB_01998, ECB_01999, ECB_02000, ECB_02001, ECB_02002, ECB_02003, ECB_02004, ECB_02005, ECB_02006, ECB_02007, ECB_02008, ECB_02009, ECB_02010, ECB_02011, ECB_02012, [ECB_02013] |
|
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| RA | 2,213,269 | C→A | K95N (AAG→AAT) | fruK ← | 1‑phosphofructokinase |
| RA | 3,250,438 | A→C | S271A (TCA→GCA) | infB ← | translation initiation factor IF‑2 |
| RA | 3,326,660 | G→A | P36L (CCA→CTA) | mreD ← | cell wall structural complex MreBCD transmembrane component MreD |
| RA | 3,497,984 | T→G | K18Q (AAG→CAG) | glgP ← | glycogen phosphorylase |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
| RA | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| RA | 4,162,521 | G→A | G556S (GGT→AGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| RA | 4,202,412 | C→T | A50T (GCG→ACG) | iclR ← | DNA‑binding transcriptional repressor |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | REL606 | 546983–547700 | 593418 | 45719–46436 | 21 [18] | [19] 20 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
| * | * | ÷ | REL606 | 593538 | 619825–619116 | 25579–26288 | 20 [19] | [18] 23 | [fepA]–[insA‑8] | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 4323213 = | 69 (1.220) | 62 (1.150) | 45/144 | 0.1 | 48.8% | coding (1119/1410 nt) | melB | melibiose:sodium symporter |
| ? | REL606 | = 4375145 | 64 (1.180) | coding (926/1338 nt) | amiB | N‑acetylmuramoyl‑l‑alanine amidase II | |||||