Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F1 I1 R1
|
18 |
56.1 |
3547650 |
96.9% |
3437672 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,202,412 |
C→T |
A50T (GCG→ACG) |
iclR ← |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,202,412 | 0 | C | T | 100.0%
| 139.1
/ NA
| 37 | A50T (GCG→ACG) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/17); total (20/17) |
GCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCG > REL606/4202340‑4202477
|
gCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAg < 1:1429394/76‑1 (MQ=255)
aCGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGtt < 2:1205384/76‑1 (MQ=255)
cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTc < 1:1019844/76‑1 (MQ=255)
cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTc > 1:1219202/1‑76 (MQ=255)
cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTc > 1:1401269/1‑76 (MQ=255)
cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTc > 2:1409127/1‑76 (MQ=255)
aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCg > 2:1353949/1‑76 (MQ=255)
aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCg > 2:1031042/1‑76 (MQ=255)
aCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCNGGCTTGTTGCGTCAGTTCCGTg < 2:238063/76‑1 (MQ=255)
gTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCcac > 1:576271/1‑76 (MQ=255)
ttGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCcaca < 2:540185/76‑1 (MQ=255)
aTTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGc < 2:970970/76‑1 (MQ=255)
aTTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGc > 1:1539849/1‑76 (MQ=255)
tgtgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCa < 1:1144768/76‑1 (MQ=255)
tgtgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCa > 2:947980/1‑76 (MQ=255)
gtgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCAt < 2:685749/76‑1 (MQ=255)
tgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCAtt < 1:1317735/76‑1 (MQ=255)
ggTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTg < 2:1714711/76‑1 (MQ=255)
cAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCg > 1:323075/1‑76 (MQ=255)
aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 2:1125338/1‑76 (MQ=255)
aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 1:290137/1‑76 (MQ=255)
ggCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGc < 2:55999/76‑1 (MQ=255)
ggTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAAt > 1:999209/1‑76 (MQ=255)
tGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATcc > 1:705340/1‑76 (MQ=255)
cGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTcc < 1:171762/76‑1 (MQ=255)
tGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAg > 2:199452/1‑76 (MQ=255)
ggAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGt > 2:213779/1‑76 (MQ=255)
gAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTa < 2:891575/76‑1 (MQ=255)
tGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAttt > 1:307805/1‑76 (MQ=255)
tGGGCAACCCGGCTTGTTGCGTCAGGTCCGCGAGTGCCCCACTGCCATTGGATCCAGCCATCCACTCCGGTAAttt > 2:786757/1‑76 (MQ=255)
gggTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTc < 2:1235348/76‑1 (MQ=255)
gggTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTc < 2:1212530/76‑1 (MQ=255)
aaCCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGc > 2:584859/1‑76 (MQ=255)
aaCCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGc > 1:1518359/1‑76 (MQ=255)
cGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcg < 1:438514/76‑1 (MQ=255)
ggCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcgc > 1:586821/1‑76 (MQ=255)
gCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCANTCCAGTAATTTCAGGCCAcgcg < 2:243301/76‑1 (MQ=255)
gCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcgcg > 1:190700/1‑76 (MQ=255)
|
GCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCG > REL606/4202340‑4202477
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
