Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A16 F1 I1 R1
|
16 |
38.1 |
2332770 |
98.7% |
2302443 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,146,135 |
T→G |
I91S (ATC→AGC) |
rpoD → |
RNA polymerase sigma factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,146,135 | 0 | T | G | 100.0%
| 158.8
/ NA
| 40 | I91S (ATC→AGC) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (8/32); total (8/32) |
TGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGG > REL606/3146067‑3146207
|
tgAAAACACCGCGGCCGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGgcgc < 2:592242/76‑1 (MQ=255)
tgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGgcgc < 2:592253/76‑1 (MQ=255)
tgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGgcgc < 2:592249/76‑1 (MQ=255)
aaaCACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACg > 1:386428/1‑76 (MQ=255)
aaCACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGa > 2:618812/1‑76 (MQ=255)
cacCGCGGACGAAGATGCTGCCGAAGCCGCGGCGCAGATGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACt < 1:1114827/76‑1 (MQ=255)
cccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTg < 1:175081/75‑1 (MQ=255)
cccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTg < 1:257059/75‑1 (MQ=255)
cccgGAAGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGAc < 1:988982/74‑1 (MQ=255)
ggACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCg < 1:1100430/76‑1 (MQ=255)
ggACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCg < 1:887964/76‑1 (MQ=255)
ggACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGAACGACTGACCCg > 1:770464/1‑76 (MQ=255)
aCGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGt < 1:952220/76‑1 (MQ=255)
aaGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACg < 2:694410/76‑1 (MQ=255)
gATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTTGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCa < 1:248783/76‑1 (MQ=255)
aTGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCAt < 1:471472/76‑1 (MQ=255)
gctgcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGt < 1:315413/76‑1 (MQ=255)
ctgcCGAAGCCTCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTa < 2:469754/76‑1 (MQ=255)
ctgcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTa < 2:93351/76‑1 (MQ=255)
gccgccGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTg < 1:384888/76‑1 (MQ=255)
gccgccGCGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTg < 2:829952/76‑1 (MQ=255)
cgccgcGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGaa < 2:671309/76‑1 (MQ=255)
ccgcGCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAAt < 1:86763/76‑1 (MQ=255)
cgcgCAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATg > 1:995228/1‑76 (MQ=255)
cgcAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATggg < 1:241812/76‑1 (MQ=255)
cgcAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATggg < 2:997486/76‑1 (MQ=255)
cgcAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATggg > 1:292780/1‑76 (MQ=255)
gcAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGc < 2:974187/76‑1 (MQ=255)
cAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCa < 2:590456/76‑1 (MQ=255)
cAGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCa < 2:1076283/76‑1 (MQ=255)
aGGTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAc < 1:795251/76‑1 (MQ=255)
gTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCg < 2:543158/76‑1 (MQ=255)
gTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCg < 2:316933/76‑1 (MQ=255)
gTGCTTTCCAGCGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCg < 1:605484/76‑1 (MQ=255)
gCGTGGAATCTGAAAGCGGTCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGtt < 2:535716/76‑1 (MQ=255)
cGTGGAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTg < 1:1137835/76‑1 (MQ=255)
tGGAATCTGAAAGCGGGCGCACGACTGACCCGTTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGAc < 1:192651/76‑1 (MQ=255)
ggAATCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGAACATGCGTGATATGCGCACCGTTGAACTGCTGccc > 2:406110/1‑76 (MQ=255)
aTCTGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgc < 1:464826/76‑1 (MQ=255)
tGAAAGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGaa > 2:433404/1‑76 (MQ=255)
aaaGCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCAGCGAAgg > 2:1119574/1‑76 (MQ=255)
|
TGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGG > REL606/3146067‑3146207
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
