breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 474,189 | +GC | coding (1117/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 702,967 | A→G | intergenic (+32/+184) | ECB_00650 → / ← kdpE | conserved hypothetical protein/DNA‑binding response regulator in two‑component regulatory system with KdpD |
RA | 1,264,346 | C→T | S161S (AGC→AGT) | hemA → | glutamyl‑tRNA reductase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 1,886,204 | G→A | intergenic (‑34/+635) | yobF ← / ← yebO | hypothetical protein/hypothetical protein |
RA | 2,110,903 | (T)7→8 | coding (285/786 nt) | ECB_01999 → | putative phage protein |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
JC JC | 3,015,771 | IS150 (–) +3 bp | coding (1192‑1194/1677 nt) | ECB_02816 → | KpsD protein |
RA | 3,146,135 | T→G | I91S (ATC→AGC) | rpoD → | RNA polymerase sigma factor |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,912,872 | Δ5 bp | coding (513‑517/1647 nt) | ilvG → | acetolactate synthase II, valine insensitive, large subunit |
RA | 4,162,470 | A→C | T539P (ACC→CCC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,163,734 | T→C | L960P (CTG→CCG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546990–547700 | 593374 | 45675–46385 | 12 [11] | [11] 12 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593429 | 619825–619116 | 25688–26397 | 12 [11] | [10] 12 | [fepA]–[insA‑8] | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8] |
* | * | ÷ | REL606 | 2032639–2032709 | 2055940–2055882 | 23174–23302 | 13 [11] | [9] 12 | [manB]–[cpsG] | [manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,wzx,rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG] |
* | * | ÷ | REL606 | 3895000 | 3901930 | 6931 | 12 [0] | [0] 47 | rbsD–[yieO] | rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 3652533 | NA (NA) | 46 (1.250) | 34/146 | 0.1 | 100% | noncoding (1443/1443 nt) | IS150 | repeat region |
? | REL606 | 3901931 = | 0 (0.000) | coding (489/1428 nt) | yieO | predicted multidrug or homocysteine efflux system |