breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 474,189 +GC coding (1117/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 702,967 A→G intergenic (+32/+184) ECB_00650 → / ← kdpE conserved hypothetical protein/DNA‑binding response regulator in two‑component regulatory system with KdpD
RA 1,264,346 C→T S161S (AGC→AGT hemA → glutamyl‑tRNA reductase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,886,204 G→A intergenic (‑34/+635) yobF ← / ← yebO hypothetical protein/hypothetical protein
RA 2,110,903 (T)7→8 coding (285/786 nt) ECB_01999 → putative phage protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
JC JC 3,015,771 IS150 (–) +3 bp coding (1192‑1194/1677 nt) ECB_02816 → KpsD protein
RA 3,146,135 T→G I91S (ATC→AGC)  rpoD → RNA polymerase sigma factor
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,912,872 Δ5 bp coding (513‑517/1647 nt) ilvG → acetolactate synthase II, valine insensitive, large subunit
RA 4,162,470 A→C T539P (ACC→CCC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,163,734 T→C L960P (CTG→CCG)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546990–547700 593374 45675–46385 12 [11] [11] 12 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593429 619825–619116 25688–26397 12 [11] [10] 12 [fepA]–[insA‑8] [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]
* * ÷ REL606 2032639–2032709 2055940–2055882 23174–23302 13 [11] [9] 12 [manB]–[cpsG] [manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,wzx,rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG]
* * ÷ REL606 3895000 3901930 6931 12 [0] [0] 47 rbsD–[yieO] rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)46 (1.250) 34/146 0.1 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system