Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
14 |
24.7 |
1576304 |
96.5% |
1521133 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,638 |
A→C |
F133C (TTC→TGC) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,638 | 0 | A | C | 100.0%
| 78.0
/ NA
| 21 | F133C (TTC→TGC) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (11/10); total (11/10) |
TGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCG > REL606/3497567‑3497709
|
tGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGgcagca > 2:765288/1‑76 (MQ=255)
tGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGgcagca < 2:488472/76‑1 (MQ=255)
aaCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGcagcaag > 1:756524/1‑76 (MQ=255)
tACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAgccgc > 1:341917/1‑76 (MQ=255)
cgaaGTCATAGCGGATGCCCTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAg > 2:573498/4‑76 (MQ=255)
gTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAgc > 2:432670/1‑75 (MQ=255)
ggATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGcc > 2:468619/1‑76 (MQ=255)
gCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCg < 2:457096/76‑1 (MQ=255)
ccGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCNGACGTCCCAGGCCACCGt > 1:619428/1‑76 (MQ=255)
gTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTa > 2:587370/1‑76 (MQ=255)
aaCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTAcc > 1:340588/1‑76 (MQ=255)
cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGc < 1:511129/76‑1 (MQ=255)
gCACCCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATc < 1:489300/76‑1 (MQ=255)
cAACCCTAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGATGCCCGGATCa < 1:653624/76‑1 (MQ=255)
cAACCCAAACGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCa > 1:121647/1‑76 (MQ=255)
aaCGTCGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTttctt > 2:416906/1‑76 (MQ=255)
cGCCAGAGAATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTtcatca < 2:472329/76‑1 (MQ=255)
gagaATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTTATCAATCAg < 2:756520/76‑1 (MQ=255)
gaATCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGct < 1:587370/76‑1 (MQ=255)
aaTCAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGctc > 1:61870/1‑76 (MQ=255)
cAAGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTc < 1:147573/76‑1 (MQ=255)
aaGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCg > 1:293337/1‑76 (MQ=255)
aaGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCg < 1:444678/76‑1 (MQ=255)
aaGGCAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCg < 2:743290/76‑1 (MQ=255)
|
TGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCG > REL606/3497567‑3497709
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
