Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
20 |
51.0 |
3438118 |
91.8% |
3156192 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,639 |
A→G |
F133L (TTC→CTC) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,639 | 0 | A | G | 100.0%
| 164.2
/ NA
| 44 | F133L (TTC→CTC) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/26); total (18/26) |
TTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCGA > REL606/3497566‑3497710
|
ttGAACATACCGTAGTCATAGCGGATGCCGTAACCGTGCACCGGCAACNCTAACGTCGCCAGAGAATCAAGGaggc < 1:1498721/76‑1 (MQ=255)
tGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGaggca < 1:549746/76‑1 (MQ=255)
aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGaggcaagc < 1:1243830/76‑1 (MQ=255)
naTACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAgcc > 1:425794/2‑76 (MQ=255)
aTACCGTAGTCATAGCGGATGCCGTAANCGCGCCCCAGCCACCCTAACGTCGCCAGAGAATCAAGGAGGCAAgccg > 1:1502402/1‑76 (MQ=255)
aCCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAgccgcc < 2:769182/76‑1 (MQ=255)
ccGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAAAAAATCAAGGAGGCAAgccgccg > 1:1119548/1‑75 (MQ=255)
tAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGAc > 2:881431/1‑76 (MQ=255)
aGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACg > 1:29263/1‑76 (MQ=255)
aGTCATAGCGGATGCCGTAACCGCGCCCCGGCAAACCTAACNTCGCCAGAGAATCAAGGANGCNAGCCGCCAGACg > 1:1396065/1‑76 (MQ=255)
aTAGCGGATGCCGTAACCGCGCCCCGGCAACCCTCACGTCGNCTGAGAATCAAGGAGGCANGCNGCCAGACGTccc > 1:1392316/1‑76 (MQ=255)
aTAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCGGCCAGACGTccc > 1:489185/1‑76 (MQ=255)
tAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCa < 1:1554110/76‑1 (MQ=255)
cGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGc > 1:956625/1‑76 (MQ=255)
ggATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGcc > 2:1294220/1‑76 (MQ=255)
ggATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGcc > 2:1149552/1‑76 (MQ=255)
gATGCNGCAACCGCGCCCCGGCAACCCTACCGTCGCCAGAGAATCAAGGAGGCAAGCCGCCNGACGTCCCAGGCCa > 2:1714087/1‑76 (MQ=255)
gCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCg < 1:609896/76‑1 (MQ=255)
ccGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGt > 1:1626441/1‑76 (MQ=255)
cGTACCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGtt < 1:997076/76‑1 (MQ=255)
cGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGtt < 2:883826/76‑1 (MQ=255)
gTACCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTa < 2:1658116/76‑1 (MQ=255)
gTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTa < 2:818773/76‑1 (MQ=255)
gTAACCGCGCCCCGGCAACCCTAACGTCACCAGAGAATAAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGCTa > 2:1092559/1‑76 (MQ=38)
tAACCGCGCCCCGGCACCCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTAc < 1:1306244/76‑1 (MQ=255)
aaCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTAcc > 2:1073040/1‑76 (MQ=255)
aCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCg < 2:861338/76‑1 (MQ=255)
cgcgCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAg < 1:306052/76‑1 (MQ=255)
gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCCGGCCACCGTTACCGAgg < 1:1703162/76‑1 (MQ=255)
gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAgg < 1:1104689/76‑1 (MQ=255)
gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAgg < 1:1351492/76‑1 (MQ=255)
ccGGCACCCCTCACGCCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCgg < 2:640616/76‑1 (MQ=37)
ggCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGAt < 2:1599019/76‑1 (MQ=255)
ggCAACCCTAACGGCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGNCACCGTTCACNAGGGCCGGAt < 2:1688627/76‑1 (MQ=25)
aaCCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCAt < 2:1114776/76‑1 (MQ=255)
ccTAACGTCGCCAGNGAATCAAGGAGGCAAGCCGCCAGACGTTCCAGGCCACCGTTACCGCGGGCCGGATCAtttt < 1:1469301/76‑1 (MQ=255)
aaCGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTttctt > 2:763937/1‑76 (MQ=255)
gCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaa < 2:1238231/76‑1 (MQ=255)
cAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatc < 2:1471742/76‑1 (MQ=255)
cAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatc < 2:888355/76‑1 (MQ=255)
cAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatc < 2:493358/76‑1 (MQ=255)
tgagaATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatca > 2:1102337/2‑76 (MQ=255)
tgagaATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatca > 2:192327/2‑76 (MQ=255)
agagAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatca < 2:956623/76‑1 (MQ=255)
gagaATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAg < 1:1216424/76‑1 (MQ=255)
agaATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGc < 1:1092561/76‑1 (MQ=255)
gaATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGct < 2:1291604/76‑1 (MQ=255)
aaTCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGctc > 2:721050/1‑76 (MQ=255)
aTCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGctct > 2:1495808/1‑76 (MQ=255)
cAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTc > 2:1263804/1‑76 (MQ=255)
aaGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCATTCAGCTCTCCg < 1:1574514/76‑1 (MQ=37)
aaGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCg > 2:625656/1‑76 (MQ=255)
aggaggCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCga > 2:504707/1‑76 (MQ=255)
|
TTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCGA > REL606/3497566‑3497710
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
