Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	14	24.7	1576304	96.5%	1521133	75.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,928,907	A→G	T231A (ACC→GCC)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,928,907	0	A	G	90.5%	62.4 / ‑1.0	21	T231A (ACC→GCC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (9/10); total (9/12)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

GTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGA  >  REL606/3928832‑3928980

gTTCTGGTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTa                                                                           >  1:123532/1‑76 (MQ=255)

   ctgctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCt                                                                        >  1:165692/1‑76 (MQ=255)

   ctgctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCt                                                                        >  1:763241/1‑76 (MQ=255)

     gctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCttt                                                                      <  2:595778/76‑1 (MQ=255)

      ctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTg                                                                     <  2:445362/76‑1 (MQ=255)

       tgAACGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCAGGTAAANGGTGAAGTTGTTGCTTCTGCCTTGGa                                                                    <  2:680757/76‑1 (MQ=255)

         aTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACg                                                                  >  2:765241/1‑76 (MQ=255)

         aTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACg                                                                  <  2:725073/76‑1 (MQ=255)

                aaCGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGc                                                           >  2:279127/1‑76 (MQ=255)

                  cGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCAt                                                         <  2:316481/76‑1 (MQ=255)

                           gtagTAACCGAGATGCAGCGTCCGGNAAAAGGAGAAGTTGTTGATTCTGCCTNTGACGAACCNGCATCTCGNCAcn                                                >  1:675443/3‑75 (MQ=255)

                              gTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTc                                             <  1:244579/76‑1 (MQ=255)

                                       aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCgg                                    >  2:408381/1‑76 (MQ=255)

                                        ggCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGa                                   <  2:439257/75‑1 (MQ=255)

                                            gCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTCACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATg                               <  1:525184/76‑1 (MQ=255)

                                             cGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATgg                              <  1:301113/76‑1 (MQ=255)

                                                    tAAAAGGTGAAGTTGTTGCTTCTGCCTTTAGCGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCga                       >  1:776808/1‑76 (MQ=255)

                                                       aaGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGaa                    <  2:767478/76‑1 (MQ=255)

                                                               gttgttGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAAc            >  1:284055/1‑76 (MQ=255)

                                                                 tgttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGc          >  1:644473/1‑76 (MQ=255)

                                                                 tgttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGc          >  2:81472/1‑76 (MQ=255)

                                                                       ttCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGtt    <  2:511364/76‑1 (MQ=255)

                                                                         cTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAGACGCCTGGTTGa  <  2:123532/76‑1 (MQ=255)

GTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGA  >  REL606/3928832‑3928980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A