Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A78 F1 I1 R1
|
16 |
49.7 |
3184232 |
95.8% |
3050494 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,907 |
A→G |
T231A (ACC→GCC) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,907 | 0 | A | G | 98.2%
| 218.1
/ ‑4.2
| 55 | T231A (ACC→GCC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (30/24); total (31/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 |
GCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTT > REL606/3928837‑3928978
|
gctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCttt > 2:266813/1‑76 (MQ=255)
ctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTg < 2:1468305/76‑1 (MQ=255)
tgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGa < 2:50936/76‑1 (MQ=255)
gATCGACGAACGTCCGGAAGAAGTAACCGGGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGAc > 2:73482/1‑76 (MQ=255)
gATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGAc > 2:792287/1‑76 (MQ=255)
tCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGa > 1:1517535/1‑76 (MQ=255)
tCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGa > 2:60088/1‑76 (MQ=255)
acgaACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAAcc < 1:884773/76‑1 (MQ=255)
acgaACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAAcc > 2:1265061/1‑76 (MQ=255)
gTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCAtc > 1:847721/1‑76 (MQ=255)
tCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCAtct > 2:1306279/1‑76 (MQ=255)
ggAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGc > 2:1054094/1‑76 (MQ=255)
gaagaaGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGcc > 2:1448900/1‑76 (MQ=255)
agaagTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCAc < 1:1181251/76‑1 (MQ=255)
agaagTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCAc > 1:481521/1‑76 (MQ=255)
aagTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGt < 2:995889/76‑1 (MQ=255)
aagTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGt > 2:635083/1‑76 (MQ=255)
gTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTc < 1:60088/76‑1 (MQ=255)
gTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTc < 1:1216624/76‑1 (MQ=255)
aaCCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAg > 1:260431/1‑76 (MQ=255)
gagaTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTg < 1:885220/76‑1 (MQ=255)
gagaTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTg < 1:674920/76‑1 (MQ=255)
aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCgg < 2:1579989/76‑1 (MQ=255)
tGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGa < 1:108658/76‑1 (MQ=255)
cAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGaaa > 1:871175/1‑76 (MQ=255)
cAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGaaa < 1:363454/76‑1 (MQ=255)
cAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGaaa > 1:55674/1‑76 (MQ=255)
cGTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATgg > 2:940697/1‑76 (MQ=255)
gTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGt < 1:253010/76‑1 (MQ=255)
gTCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGt < 1:1447594/76‑1 (MQ=255)
tCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTg > 2:1017938/1‑76 (MQ=255)
tCTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTg < 2:1060425/76‑1 (MQ=255)
cTGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTCGCGGAAATGGTGa > 1:1549486/1‑76 (MQ=255)
tGGTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGAt > 2:1184400/1‑76 (MQ=255)
ggTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATc < 1:1403885/76‑1 (MQ=255)
gTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCg < 2:105632/76‑1 (MQ=255)
gTAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCg > 1:346293/1‑76 (MQ=255)
tAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCga > 1:1259553/1‑76 (MQ=255)
tAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCga > 1:638394/1‑76 (MQ=255)
tAAAAGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCga < 1:68960/76‑1 (MQ=255)
aaaaGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCgag < 2:878719/76‑1 (MQ=255)
aaaaGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCgag < 1:767709/76‑1 (MQ=255)
aaaaGGTGAAGTTGTTGCTTCCGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCgag > 1:735415/1‑76 (MQ=255)
aaGGTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGaa > 1:425120/1‑76 (MQ=255)
aaGGGGATGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGGTCAGGTTGCGGGAATGGTGATCGATaa > 2:789370/1‑76 (MQ=255)
ggTGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAgg < 1:418475/76‑1 (MQ=255)
tGAAGTTGTTGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGcc > 1:1000448/1‑76 (MQ=255)
gttgttGCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAAc < 2:1320115/76‑1 (MQ=255)
ttgttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCaaccc > 1:796375/1‑73 (MQ=255)
ttgttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACg > 1:222119/1‑76 (MQ=255)
tgttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGc > 2:1341756/1‑76 (MQ=255)
ttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCt < 2:186524/76‑1 (MQ=255)
ttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCt < 2:1250272/76‑1 (MQ=255)
ttgCTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCt > 1:765734/1‑76 (MQ=255)
cTTCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGt > 1:6068/1‑76 (MQ=255)
ttCTGCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGtt < 2:432085/76‑1 (MQ=255)
|
GCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTT > REL606/3928837‑3928978
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
