Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A22 F1 I1 R1
|
17 |
44.7 |
2806446 |
96.5% |
2708220 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,016,003 |
G→A |
W475* (TGG→TAG) |
ECB_02816 → |
KpsD protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,016,003 | 0 | G | A | 100.0%
| 163.1
/ NA
| 43 | W475* (TGG→TAG) | ECB_02816 | KpsD protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/19); total (24/19) |
GTCATCAATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTGGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTAACG > REL606/3015930‑3016071
|
gTCATCAANATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGc < 1:1235103/76‑1 (MQ=255)
caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg > 2:954090/1‑76 (MQ=255)
caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg > 1:734654/1‑76 (MQ=255)
caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg < 2:618642/76‑1 (MQ=255)
caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg > 1:498625/1‑76 (MQ=255)
ttCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTAt > 2:404534/1‑76 (MQ=255)
tCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATg > 1:192754/1‑76 (MQ=255)
ccGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGa < 1:137805/76‑1 (MQ=255)
cGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGac > 1:99378/1‑76 (MQ=255)
cGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGac > 1:501082/1‑76 (MQ=255)
gagaAAACATCGCTGATTATGGTTCATGGGGAAGTGCTGTTCCCGAACGCTGTGAGCTAGCAGAAGGGTATGacca < 1:72910/76‑1 (MQ=255)
gagaAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGacca < 1:483662/76‑1 (MQ=255)
cATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGa < 1:790209/76‑1 (MQ=255)
tCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGAtt < 2:432489/76‑1 (MQ=255)
tGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACAt > 1:256640/1‑76 (MQ=255)
tGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACAt > 1:701653/1‑76 (MQ=255)
tGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACAt > 1:747862/1‑76 (MQ=255)
gATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATc < 2:498625/76‑1 (MQ=255)
gATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATc > 1:793359/1‑76 (MQ=255)
aTTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCg < 1:212161/76‑1 (MQ=255)
aTGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCgaga > 2:665309/1‑76 (MQ=255)
tGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGaa > 2:846161/1‑76 (MQ=255)
ggTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGCAGGGTATGACCACCGAGGATTAAATCGAGaaa > 2:412709/1‑76 (MQ=255)
gTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAAt > 1:1106485/1‑76 (MQ=255)
ttCATGGCGAAGTGCTGTTCCCGAAAGCNGTGAGCTAGAAGGAGGGTGTGACCACCGAGGATTACATCGAGAAAtg < 1:125151/76‑1 (MQ=255)
cATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAAtgtg > 1:335853/1‑76 (MQ=255)
aTGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATgtgg < 2:1225900/76‑1 (MQ=255)
gCGAAGTGCTGTTCCCGAACGCGGTGAGCTATCAGAAGGANNTGACCACCGANNACTACATCGAGAAATGtggtgg > 1:1258131/1‑76 (MQ=255)
gAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGcc > 2:127783/1‑76 (MQ=255)
aaGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGATCACCGAGGATTACATCGAGAAATTTGGTGGCCt < 2:1194598/76‑1 (MQ=255)
aaGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCt < 1:469089/76‑1 (MQ=255)
gTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGa > 1:645010/1‑76 (MQ=255)
gTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGa > 1:488854/1‑76 (MQ=255)
tGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGAc > 2:912828/1‑76 (MQ=255)
gCTGTGCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACg < 2:1074956/76‑1 (MQ=255)
cTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGc < 1:888187/76‑1 (MQ=255)
tGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGNCCTGACGCa < 1:36878/76‑1 (MQ=255)
tGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCa < 2:867887/76‑1 (MQ=255)
gTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCaa > 1:708476/1‑76 (MQ=255)
ttCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCaaa > 2:298531/1‑76 (MQ=255)
cccGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCaaaaa > 2:836815/1‑76 (MQ=255)
cgGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTaa > 2:519978/1‑76 (MQ=255)
cgGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTaa < 1:748278/76‑1 (MQ=255)
gTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTAACg < 1:1043067/76‑1 (MQ=255)
|
GTCATCAATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTGGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTAACG > REL606/3015930‑3016071
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
