Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A22 F1 I1 R1
|
17 |
44.7 |
2806446 |
96.5% |
2708220 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,164,745 |
A→C |
D1297A (GAC→GCC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,164,745 | 0 | A | C | 100.0%
| 165.8
/ NA
| 41 | D1297A (GAC→GCC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (20/21); total (20/21) |
GAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGA > REL606/4164675‑4164817
|
gAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTNATGCCGTGa < 1:31484/76‑1 (MQ=255)
gAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGa > 2:1129557/1‑76 (MQ=255)
aGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATNCTCACCTTTAAGTCTGATGCCGTGAAc < 1:1400102/76‑1 (MQ=255)
gggNGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATNCTCACCGTTAAGTCTGATGCCGTGAACGGTCg < 2:1305088/76‑1 (MQ=255)
ggCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGt < 1:540705/76‑1 (MQ=255)
gcgcTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTa < 1:621032/76‑1 (MQ=255)
gcgcTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTa < 2:1396515/76‑1 (MQ=255)
cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg < 2:421514/71‑1 (MQ=255)
cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg > 1:421514/1‑71 (MQ=255)
cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg < 2:302618/71‑1 (MQ=255)
cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg > 1:302618/1‑71 (MQ=255)
cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCa > 1:144310/1‑76 (MQ=255)
ggAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGNCGTGAACGGTCGTACCAAg < 1:1302050/76‑1 (MQ=255)
aaGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGAt > 2:952381/1‑76 (MQ=255)
aGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCCTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATg > 2:1029111/1‑76 (MQ=255)
cGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATaaa < 2:15522/76‑1 (MQ=255)
gcgcAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATaaaaa < 1:1221651/76‑1 (MQ=255)
cgcAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAAc > 1:192140/1‑76 (MQ=255)
cagcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACAt > 1:779619/1‑76 (MQ=255)
cagcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTCAACGGTCGTACCAAGATGTATAAAAACAt > 2:376809/1‑76 (MQ=255)
agcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATc < 2:991008/76‑1 (MQ=255)
agcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATc < 1:1368301/76‑1 (MQ=255)
aTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTg > 2:1372622/1‑76 (MQ=255)
tACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTgg < 2:737524/76‑1 (MQ=255)
cacCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGAc < 2:160336/76‑1 (MQ=255)
acCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACg > 2:551281/1‑76 (MQ=255)
acCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACg > 2:763171/1‑76 (MQ=255)
cccTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACgg > 1:256999/1‑76 (MQ=255)
cAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAAcc > 1:356376/1‑76 (MQ=255)
cAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTAGTACCAAGATGTATAAAAACATCGTGGACGGCAAcc < 1:871296/76‑1 (MQ=255)
aGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAAcct > 1:304731/1‑75 (MQ=255)
ggAAATGCTCACCGTTAAGTCTGATTCCNTGAACGGTCCAAACAAGAGGTATAAAAACATCGTGGACGGCAACCAt < 1:122684/76‑1 (MQ=255)
ggAAACGCCCACCGTTAAGTCTGATCCCGTGAACGGTCGTACCAAGATGTATAAAACCACCGTGGACGGCAACCAt < 2:1030131/76‑1 (MQ=255)
aaaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCa > 1:383485/1‑76 (MQ=255)
aaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAg > 2:808035/1‑76 (MQ=255)
aaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAg < 1:969324/76‑1 (MQ=255)
aaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAg > 2:808037/1‑76 (MQ=255)
tGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGAt < 1:278979/76‑1 (MQ=255)
gCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATg < 1:413928/76‑1 (MQ=255)
cTCACCGTTAAGTCNGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATgn < 2:193693/76‑2 (MQ=255)
tAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGc > 2:561683/1‑76 (MQ=255)
aaGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCa < 1:749770/76‑1 (MQ=255)
gTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATg > 1:1061266/1‑76 (MQ=255)
tgatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCa < 2:658360/76‑1 (MQ=255)
tgatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCa > 2:291587/1‑76 (MQ=255)
gatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCGTCAGATGGAGCCTGGCATGCCAg > 2:1025348/1‑76 (MQ=255)
gatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAg < 1:835859/76‑1 (MQ=255)
gatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAg > 1:568506/1‑76 (MQ=255)
atgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGa < 1:251925/76‑1 (MQ=255)
atgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGa > 1:818225/1‑76 (MQ=255)
|
GAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGA > REL606/4164675‑4164817
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
