Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A40 F1 I1 R1
|
16 |
59.7 |
3732628 |
97.2% |
3628114 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,252,006 |
A→G |
V252A (GTA→GCA) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,252,006 | 0 | A | G | 100.0%
| 157.8
/ NA
| 40 | V252A (GTA→GCA) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/20); total (20/20) |
ACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCG > REL606/3251935‑3252077
|
acaGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCa < 2:1300415/76‑1 (MQ=255)
caGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCaa > 2:368288/1‑76 (MQ=255)
gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCt < 2:1342045/76‑1 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCgg > 2:687651/1‑76 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCgg > 2:66330/1‑76 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGCAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCgg < 1:1133276/76‑1 (MQ=255)
gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGa > 1:4312/1‑76 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCg < 2:494739/76‑1 (MQ=255)
cGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGa > 2:1166711/1‑76 (MQ=255)
cGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGa > 2:1831184/1‑76 (MQ=255)
gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGAt < 1:1825114/76‑1 (MQ=255)
cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCACCACGCATACCAGCGCNAGCACCTACCGGATCGATACg > 2:294473/1‑76 (MQ=255)
gCCACCCAGTTCAGTAGACACCGCCTGTACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGt > 2:1837494/1‑76 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGttt > 2:1057392/1‑76 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGttt < 1:1123419/76‑1 (MQ=255)
cccAGTTCAGTAGACCCCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTAt < 2:1655354/76‑1 (MQ=255)
cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCg < 2:732984/76‑1 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTgg < 1:223009/76‑1 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTgg < 1:485830/76‑1 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTgg > 1:1159334/1‑76 (MQ=255)
tAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGANCGATACGTTTATCGTTGGtttt < 2:256471/76‑1 (MQ=255)
tAGACACCGCCGGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGtttt < 2:1278010/76‑1 (MQ=255)
aGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTc < 2:853031/76‑1 (MQ=255)
acCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg < 1:308396/76‑1 (MQ=255)
acCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg > 1:1531336/1‑76 (MQ=255)
acCGCCTGAACACGCGCGCAACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg > 1:1659660/1‑76 (MQ=255)
gCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGa > 2:1463257/1‑76 (MQ=255)
gCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGa > 1:151145/1‑76 (MQ=255)
tGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt > 1:1724977/1‑76 (MQ=255)
tGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt > 2:1142037/1‑76 (MQ=255)
gAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGCTACGTTTATCGTTGGTTTTCACCGCGAtttt > 2:1346277/1‑76 (MQ=255)
gAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt > 2:1659202/1‑76 (MQ=255)
aaCACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTc < 2:791754/76‑1 (MQ=255)
acGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCa > 1:1236578/1‑76 (MQ=255)
gcgcgcCGCGCATGCCTGCGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg < 2:163172/76‑1 (MQ=255)
gcgcgcCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg < 2:1026898/76‑1 (MQ=255)
cGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg < 1:515753/76‑1 (MQ=255)
aTACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt < 2:1132608/76‑1 (MQ=255)
aTACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt < 1:665610/76‑1 (MQ=255)
tACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAta > 2:1047268/1‑75 (MQ=255)
aCCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcg < 2:1756002/76‑1 (MQ=255)
|
ACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCG > REL606/3251935‑3252077
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
