Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A40 F1 I1 R1
|
16 |
59.7 |
3732628 |
97.2% |
3628114 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,536 |
G→A |
A148V (GCA→GTA) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,536 | 0 | G | A | 100.0%
| 120.5
/ NA
| 33 | A148V (GCA→GTA) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/24); total (9/24) |
CAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGC > REL606/3328465‑3328606
|
cAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGc < 2:273411/76‑1 (MQ=255)
tACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGccct > 2:995422/1‑74 (MQ=255)
cccccaccGATATCAACCACCATAGAGCCGGTCACTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCg < 1:770697/73‑1 (MQ=255)
caccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCgg > 1:523266/1‑76 (MQ=255)
ccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGttctt < 2:1734009/76‑1 (MQ=255)
gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCa < 2:1802581/76‑1 (MQ=255)
atCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATc < 1:1782124/76‑1 (MQ=255)
tCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCa < 1:1502916/76‑1 (MQ=255)
acAACCACCATAGACCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCa < 1:1155923/75‑1 (MQ=255)
accaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGa < 1:1760855/76‑1 (MQ=255)
tAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCCTCGGGTCTTCCACCACGAAGACTTc > 2:377441/1‑76 (MQ=255)
aGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCCTCGGTTCTTCAATCAGGAAGACTTcc > 1:979166/1‑75 (MQ=255)
aGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCa < 1:319680/76‑1 (MQ=255)
aGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGGTCTTCAATCAGGAAGACTTCa > 2:809997/1‑76 (MQ=255)
ggTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCa < 1:1761462/76‑1 (MQ=255)
ggTCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCa < 2:892736/76‑1 (MQ=255)
tCGCTTCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCAcc < 1:1361729/76‑1 (MQ=255)
tCAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGGTCTTCAATCAGGAAGACTTCACGGGCACCAgcgc > 2:432082/1‑76 (MQ=255)
cAGAAACCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGcc < 1:1251473/76‑1 (MQ=255)
aaaCCGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCATGGGCACCAGCGCCCTg < 2:872053/76‑1 (MQ=255)
cGGCAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGcgcg < 2:877137/76‑1 (MQ=255)
cGGCAGGCCAGCACCAATTACGGCAGCCATAGGTTCTTCAATCAGGAAAACTTCACGGGCACCAGCGCCCTGcgcg > 1:164667/1‑76 (MQ=255)
cAGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGAt < 2:623338/76‑1 (MQ=255)
aGGCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGAtt < 2:1289549/76‑1 (MQ=255)
ggCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTc < 1:88503/76‑1 (MQ=255)
ggCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTc < 2:644251/76‑1 (MQ=255)
ggCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTc < 2:833112/76‑1 (MQ=255)
gCCAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCa < 2:696061/76‑1 (MQ=255)
cAGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACg < 1:660077/76‑1 (MQ=255)
aGCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGa < 1:1780359/76‑1 (MQ=255)
gCACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGaa < 2:887635/76‑1 (MQ=255)
cACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAAt < 2:262207/76‑1 (MQ=255)
cACCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAAt > 2:413822/1‑76 (MQ=255)
aCCAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGTGCGGATTCACGAAtt < 2:1799265/76‑1 (MQ=255)
cAATTACGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCCGCGCGGGTTCACGAAATgg > 1:15299/1‑75 (MQ=255)
|
CAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGC > REL606/3328465‑3328606
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
