Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F1 I1 R1
|
18 |
50.6 |
3235256 |
96.4% |
3118786 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,543 |
C→T |
A146T (GCT→ACT) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,543 | 0 | C | T | 100.0%
| 151.2
/ NA
| 41 | A146T (GCT→ACT) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/20); total (21/20) |
GTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTC > REL606/3328470‑3328615
|
gTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCa < 1:1344447/76‑1 (MQ=255)
accaccaccGATATCAACCACCATAGAACCGGTNGCTTCAGAAACCGGCAGGCCAGNACCAATTGCGNCAGTCATc > 2:1360626/1‑76 (MQ=255)
accaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATc < 1:642123/76‑1 (MQ=255)
accaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATc > 2:179362/1‑76 (MQ=255)
ccaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCg < 2:732614/76‑1 (MQ=255)
ccaccGATNTCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGtt < 1:1415297/76‑1 (MQ=255)
ccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGtt < 2:594207/76‑1 (MQ=255)
cGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACGAAGTGAGGCAGCCATCGGctcttc > 1:1533611/1‑76 (MQ=255)
gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCa > 1:691245/1‑76 (MQ=255)
gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCa < 1:1019925/76‑1 (MQ=255)
gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCa < 2:1197542/76‑1 (MQ=255)
atatCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCaa < 1:1085418/76‑1 (MQ=255)
atatCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCaa < 1:1343745/76‑1 (MQ=255)
atatCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCAGCAGTCATCGGTTCTTCaa < 2:1284167/76‑1 (MQ=255)
atCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATc > 1:955771/1‑76 (MQ=255)
tCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCa > 2:1497988/1‑76 (MQ=255)
aaCCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAgg > 1:551117/1‑76 (MQ=255)
ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTTAATCAGGAAGAc > 1:741717/1‑76 (MQ=255)
ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGAc > 2:396770/1‑76 (MQ=255)
caTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACt > 1:563239/1‑76 (MQ=255)
caTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACt > 1:636957/1‑76 (MQ=255)
caTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACt > 1:1209701/1‑76 (MQ=255)
aTAGAACCCGTCGCTTCAGAAACCGGCAGGCCAGCACCCATTGCCGGGATCATCGGTTTCTCAACCACGAAGagct > 1:1464937/1‑73 (MQ=255)
aaCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACg > 2:639011/1‑76 (MQ=255)
ggTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCa > 1:9219/1‑76 (MQ=255)
cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATNGGTTCTTCAATCAGGAAGACTTCACGGGCACCa < 2:1376526/76‑1 (MQ=255)
cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCa < 1:488448/76‑1 (MQ=255)
cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCa < 2:1052611/76‑1 (MQ=255)
gtttCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAg < 1:795402/74‑1 (MQ=255)
gCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCTCGGGCACCAg < 1:1497988/76‑1 (MQ=255)
aGAAACCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGccc < 1:865646/76‑1 (MQ=255)
aaaCCGGCAGGCCAGCACCNATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTg > 1:1504476/1‑76 (MQ=255)
aaCCGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTgc > 1:791648/1‑76 (MQ=255)
ccGGCAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAAACAGGAAGACTTCACGGGCACCAGTGCCCTgcgc < 2:1381490/76‑1 (MQ=255)
cAGGCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGAt > 1:159142/1‑76 (MQ=255)
ggCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTc < 1:744575/76‑1 (MQ=255)
ggCCAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTc < 1:1359248/76‑1 (MQ=255)
cAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACg > 2:1271458/1‑76 (MQ=255)
cAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACg > 1:1497390/1‑76 (MQ=255)
cAGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACg > 1:1301178/1‑76 (MQ=255)
aGCACCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGATTTCACGGGCACCAGCGCCCTGCGCGGATTCACGa > 2:802661/1‑76 (MQ=255)
aCCAATTGCGGCAGTCATCGGTTCTTCAATCAGGAANACTTCAGGGGCACCAGCGCCCTGCGCGGATTCACNAAtt > 1:193455/1‑76 (MQ=255)
ccAATTGCGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTg < 2:523506/76‑1 (MQ=255)
cGGCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcggcg > 1:1397923/1‑76 (MQ=255)
gCAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGtt > 2:762877/1‑76 (MQ=255)
cAGTCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTc > 2:958871/1‑76 (MQ=255)
|
GTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTC > REL606/3328470‑3328615
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
