Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A131 F1 I1 R1
|
13 |
244.3 |
15300636 |
95.6% |
14627408 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,251,805 |
C→G |
G319A (GGC→GCC) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,251,805 | 0 | C | G | 99.0%
| 825.5
/ ‑3.2
| 202 | G319A (GGC→GCC) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base G (79/121); total (79/123) |
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 |
Only 100 of 206 total aligned reads displayed.
GTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAG > REL606/3251732‑3251877
|
gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa > 2:6123668/1‑76 (MQ=255)
gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa < 1:5461463/76‑1 (MQ=255)
gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa < 1:2802772/76‑1 (MQ=255)
gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa < 1:2385810/76‑1 (MQ=255)
tcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCaa > 1:4792932/1‑76 (MQ=255)
cgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAt > 1:7354219/1‑76 (MQ=255)
cgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAt < 1:6475797/76‑1 (MQ=255)
cgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAt > 2:3082045/1‑76 (MQ=255)
gtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATc < 2:6731965/76‑1 (MQ=255)
tcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCg < 1:7255305/76‑1 (MQ=255)
tcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCg < 2:294922/76‑1 (MQ=255)
cccaCGGTCATCGCGTTGAGTTCCCACCCGCCCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCg < 1:6838621/73‑1 (MQ=255)
aaCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGcc > 1:7308928/1‑76 (MQ=255)
aaCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGcc > 1:4496289/1‑76 (MQ=255)
aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 1:1122221/76‑1 (MQ=255)
aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 2:1978344/76‑1 (MQ=255)
aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 2:4269395/76‑1 (MQ=255)
aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt > 1:2022991/1‑76 (MQ=255)
aCGGTCATCACGTTGAGTGCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 2:4416716/76‑1 (MQ=255)
ggTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTgc > 2:3967710/1‑76 (MQ=255)
catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 2:72033/1‑76 (MQ=255)
catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 2:1414952/1‑76 (MQ=255)
catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 1:4318408/1‑76 (MQ=255)
catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 1:5674604/1‑76 (MQ=255)
gtcaCGTTGAGTCCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCa < 2:5302754/75‑1 (MQ=255)
atcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCa < 1:5731971/76‑1 (MQ=255)
tcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAg > 1:4887368/1‑76 (MQ=255)
caCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAgg > 2:1194156/1‑76 (MQ=255)
caCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAgg > 1:3894083/1‑76 (MQ=255)
cGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGtt < 2:5788415/76‑1 (MQ=255)
cGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGtt < 1:5540029/76‑1 (MQ=255)
gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTa > 2:2446273/1‑76 (MQ=255)
tGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTAcc > 1:6878640/1‑76 (MQ=255)
gAGTTCCCACCCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCg < 1:5938321/76‑1 (MQ=255)
gAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCg < 1:5079142/76‑1 (MQ=255)
aGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCgg > 1:5122585/1‑76 (MQ=255)
ttCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCt < 1:35369/76‑1 (MQ=255)
ttCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCt > 1:3507839/1‑76 (MQ=255)
ttCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCt > 1:1198990/1‑76 (MQ=255)
tCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCtt > 2:110999/1‑76 (MQ=255)
cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTc < 2:5244646/76‑1 (MQ=255)
cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTc > 2:5908335/1‑76 (MQ=255)
ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCtttc > 1:674618/1‑74 (MQ=255)
cAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCaa < 1:2477723/76‑1 (MQ=255)
cAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAGCGCCTGCGCCAGGTTACCGGCTTCaa > 1:798812/1‑76 (MQ=255)
acccGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAc < 1:7009822/74‑1 (MQ=255)
aaCCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAc < 1:6665949/76‑1 (MQ=255)
gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCg > 1:5785032/1‑76 (MQ=255)
cTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAAGGCGa > 1:1403636/1‑76 (MQ=255)
tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGat < 2:1254667/76‑1 (MQ=255)
tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGat < 2:3346965/76‑1 (MQ=255)
tCACCTGGGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGat < 1:1982735/76‑1 (MQ=255)
cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata < 1:5049553/76‑1 (MQ=255)
cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata > 1:4010157/1‑76 (MQ=255)
cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata > 2:1737180/1‑76 (MQ=255)
cAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata < 1:1011573/76‑1 (MQ=255)
aGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAAGGCGgtat > 1:494033/1‑76 (MQ=255)
tGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCa < 1:3586265/76‑1 (MQ=255)
gCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCAt < 2:3603367/76‑1 (MQ=255)
aacccAGCCGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgt < 1:3992205/73‑1 (MQ=255)
aGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgtg < 1:1635321/76‑1 (MQ=255)
ccAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgtgtg < 1:712111/76‑1 (MQ=255)
aGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGtt > 1:714814/1‑76 (MQ=255)
gcGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTa < 1:3417141/75‑1 (MQ=255)
aCGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTa < 1:261526/76‑1 (MQ=255)
aCGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTa < 1:7483594/76‑1 (MQ=255)
cGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt < 1:1582561/76‑1 (MQ=255)
gCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATc < 1:1283684/76‑1 (MQ=255)
gCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATc < 2:4848553/76‑1 (MQ=255)
ccGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:6481659/75‑1 (MQ=255)
ccGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:2656183/75‑1 (MQ=255)
aCGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCCTGGGGTGTTTATCtt > 2:5604487/1‑76 (MQ=255)
aCGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:5343336/76‑1 (MQ=255)
aCGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:4544783/76‑1 (MQ=255)
cGTTTTGGCCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTc < 2:2835042/76‑1 (MQ=255)
cGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATTGTGTGTTTATCTTc > 1:4133174/1‑76 (MQ=255)
cGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTc < 2:4079923/76‑1 (MQ=255)
gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCttta > 2:6945911/1‑74 (MQ=255)
gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa < 2:7549554/76‑1 (MQ=255)
gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa > 2:728978/1‑76 (MQ=255)
gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa > 1:1475545/1‑76 (MQ=255)
gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa < 1:194049/76‑1 (MQ=255)
ttCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCAt < 1:2317224/76‑1 (MQ=255)
tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTTAACGGCGATATCCATGGTGTGTTTATCTTCATc > 1:2172348/1‑76 (MQ=255)
tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGGTATCCCTGGTGTGTTTATCTTCAtt > 1:6585093/1‑75 (MQ=255)
tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATc < 1:4665013/76‑1 (MQ=255)
tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATc < 1:6106038/76‑1 (MQ=255)
gACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac < 1:423621/76‑1 (MQ=255)
aCCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacc < 2:4997262/76‑1 (MQ=255)
aCCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacc < 2:2130086/76‑1 (MQ=255)
ccGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCCTGGGGGGTTTATCTTCATccacca > 1:7597250/1‑76 (MQ=255)
ccGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacca < 2:3559829/76‑1 (MQ=255)
ccGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATATTCATccacca > 1:939110/1‑76 (MQ=255)
cGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCcaccac > 2:3774887/1‑76 (MQ=255)
gTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCCCCACg > 1:1677133/1‑76 (MQ=255)
gTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACg < 1:4337624/76‑1 (MQ=255)
gTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACg < 2:516388/76‑1 (MQ=255)
tACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGAt < 1:3018371/76‑1 (MQ=255)
tACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGGGTGTTTATCTTCATTCACCACGAt > 1:4507605/1‑76 (MQ=255)
cGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAg < 1:6319162/76‑1 (MQ=255)
|
GTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAG > REL606/3251732‑3251877
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
