Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A75 F1 I1 R1
|
16 |
50.8 |
3316832 |
95.7% |
3174208 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,951 |
A→G |
S10P (TCC→CCC) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,951 | 0 | A | G | 95.5%
| 260.2
/ 1.5
| 67 | S10P (TCC→CCC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (40/24); total (41/26) |
| Fisher's exact test for biased strand distribution p-value = 5.55e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 |
TTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCG > REL606/3328878‑3329021
|
ttCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa < 2:866807/76‑1 (MQ=255)
ttCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa > 1:1156165/1‑76 (MQ=255)
ttCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa > 2:320427/1‑76 (MQ=255)
aCGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGc > 2:820798/1‑76 (MQ=255)
aCGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGc > 2:1612931/1‑76 (MQ=255)
aCGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGc > 2:1168427/1‑76 (MQ=255)
cGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGcc < 1:489397/76‑1 (MQ=255)
gATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCa > 2:373138/1‑76 (MQ=255)
aTGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCAc > 1:198797/1‑76 (MQ=255)
tgtcTTGTTCTTTTAGTTAAATGAGGGTGTTCGCAGTTCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACg < 2:1387399/73‑1 (MQ=255)
ccTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaa > 1:564137/1‑76 (MQ=255)
ccTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaa < 1:411470/76‑1 (MQ=255)
ccTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaa > 1:969454/1‑76 (MQ=255)
ccTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaa < 2:1203979/76‑1 (MQ=255)
cTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaaa > 1:1331221/1‑76 (MQ=255)
gTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAttt > 1:858630/1‑76 (MQ=255)
tCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtttt > 1:1337760/1‑76 (MQ=255)
ccTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAACttttt > 1:293831/1‑76 (MQ=255)
ccTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAttttt > 1:293819/1‑76 (MQ=255)
cTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtttttt > 1:902170/1‑76 (MQ=255)
tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa > 2:149792/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 1:1609562/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 1:1612778/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 1:933182/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 1:752102/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 1:665169/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 1:1041827/1‑76 (MQ=255)
cATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACac > 2:433383/1‑75 (MQ=255)
tAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATAc < 1:1064735/76‑1 (MQ=255)
aaTGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTa < 1:684000/76‑1 (MQ=255)
aTGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTaa < 1:178053/76‑1 (MQ=255)
gAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAgg > 2:505743/1‑76 (MQ=255)
gAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAgg < 1:1591985/76‑1 (MQ=255)
aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAggg > 2:1101476/1‑76 (MQ=255)
gggTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGa < 2:1421194/76‑1 (MQ=255)
gTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATa < 1:550462/76‑1 (MQ=255)
tATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATaa > 2:1524256/1‑76 (MQ=255)
aTTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAAt > 2:170315/1‑76 (MQ=255)
aTTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAAt > 2:163756/1‑76 (MQ=255)
cGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCt > 2:224528/1‑76 (MQ=255)
cGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCt > 1:571906/1‑76 (MQ=255)
gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg < 1:1130432/76‑1 (MQ=255)
gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg > 1:1374797/1‑76 (MQ=255)
taGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGa > 2:1025146/2‑76 (MQ=255)
aGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaa < 1:135914/76‑1 (MQ=255)
aGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaa < 2:1168382/76‑1 (MQ=255)
gtgcccAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa < 2:1439796/73‑1 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 1:1374087/1‑76 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa < 2:1067699/76‑1 (MQ=255)
tACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAg > 2:894834/1‑76 (MQ=255)
aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:1474558/1‑76 (MQ=255)
aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:699312/1‑76 (MQ=255)
aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 2:177639/1‑76 (MQ=255)
cccAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCt < 1:44379/76‑1 (MQ=255)
aGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTggg < 1:1287688/76‑1 (MQ=255)
aGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTggg < 1:1328818/76‑1 (MQ=255)
aGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTggg < 1:204216/76‑1 (MQ=255)
tCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCg < 2:584661/76‑1 (MQ=255)
tCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCg > 1:1558427/1‑76 (MQ=255)
cAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCgg < 2:1180116/76‑1 (MQ=255)
aaTGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGga < 2:19207/76‑1 (MQ=255)
tGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaa < 1:189576/76‑1 (MQ=255)
tgACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaa < 2:46695/75‑1 (MQ=255)
ggACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaa > 1:497844/1‑76 (MQ=255)
aCAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga < 1:1430807/76‑1 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa > 2:1626276/1‑76 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa > 2:1614445/1‑76 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa > 1:148916/1‑76 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa > 2:1260576/1‑76 (MQ=255)
aaGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaaa > 2:158316/1‑76 (MQ=255)
gTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAAt < 1:1622236/76‑1 (MQ=255)
cATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATcc > 2:1360436/1‑76 (MQ=255)
aTTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCg < 2:1174774/76‑1 (MQ=255)
|
TTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCG > REL606/3328878‑3329021
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
