Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A91 F1 I1 R1
|
18 |
55.4 |
3661525 |
95.1% |
3482110 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,951 |
A→G |
S10P (TCC→CCC) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,951 | 0 | A | G | 95.2%
| 232.5
/ 1.8
| 62 | S10P (TCC→CCC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (33/26); total (34/28) |
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 |
TTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCG > REL606/3328878‑3329021
|
ttCAATACGATGCCTTGTCCTTTTACATAAATGAGTGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa > 1:1329226/1‑76 (MQ=255)
ttCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa < 2:424100/76‑1 (MQ=255)
aTACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACAt > 2:1050395/1‑76 (MQ=255)
aTACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAt < 1:1813140/76‑1 (MQ=255)
tACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTTATTGGGAAACagg > 2:836558/1‑74 (MQ=255)
tACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATg < 2:501404/76‑1 (MQ=255)
aCGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGc < 1:1503930/76‑1 (MQ=255)
aTGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCAc < 1:974442/76‑1 (MQ=255)
aTGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCAc > 1:423901/1‑76 (MQ=255)
gCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGa > 2:1779596/1‑76 (MQ=255)
cTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaaa > 1:1058261/1‑76 (MQ=255)
tGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtt > 2:980977/1‑76 (MQ=255)
tGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtt > 1:767100/1‑76 (MQ=255)
gTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAttt > 1:581802/1‑76 (MQ=255)
gTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAttt > 1:517079/1‑76 (MQ=255)
tCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtttt < 1:97889/76‑1 (MQ=255)
ttttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTc < 1:1042830/76‑1 (MQ=255)
tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 2:1545278/76‑1 (MQ=255)
tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 1:1602444/76‑1 (MQ=255)
tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 2:1580660/76‑1 (MQ=255)
tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 1:634570/76‑1 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 2:845035/1‑76 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc < 1:1356793/76‑1 (MQ=255)
tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc < 2:672119/76‑1 (MQ=255)
cATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACAt > 1:371214/1‑76 (MQ=255)
aTAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATa < 2:940848/76‑1 (MQ=255)
aaaTGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACt > 1:1729462/1‑76 (MQ=255)
tGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGCAATTTTTTCAACATACTAAg > 2:1066527/1‑76 (MQ=255)
gAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAgg < 1:265464/76‑1 (MQ=255)
gAGGGTATTCGCAGTACCCAGGTC‑ATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 2:1441551/1‑76 (MQ=255)
aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg < 1:718404/76‑1 (MQ=255)
aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 1:948300/1‑76 (MQ=255)
aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 1:1563078/1‑76 (MQ=255)
aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 2:1183565/1‑76 (MQ=255)
ggTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGAt < 2:51300/76‑1 (MQ=255)
gTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATa > 1:651746/1‑76 (MQ=255)
aTTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAAt > 1:906320/1‑76 (MQ=255)
gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg < 1:1241772/76‑1 (MQ=255)
gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg > 1:1288108/1‑76 (MQ=255)
gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg > 2:713969/1‑76 (MQ=255)
gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg < 1:297959/76‑1 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa < 1:1353139/76‑1 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 1:553434/1‑76 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa < 1:1368475/76‑1 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 2:188349/1‑76 (MQ=255)
gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 1:1259374/1‑76 (MQ=255)
aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:110329/1‑76 (MQ=255)
aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:1378535/1‑76 (MQ=255)
aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:827987/1‑76 (MQ=255)
ccAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTg < 2:51884/76‑1 (MQ=255)
ggTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTgggg < 2:1668093/76‑1 (MQ=255)
tCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCg > 2:687323/1‑76 (MQ=255)
aaTGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGga > 1:410308/1‑76 (MQ=255)
tGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaa < 2:3428/76‑1 (MQ=255)
tgACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCTgaaca > 2:1829690/2‑74 (MQ=255)
tgACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaa > 2:1348611/2‑76 (MQ=255)
ggACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACACACTAAGGGATAATCCTGTAATCTGGGGCGgaaaa > 2:527321/1‑76 (MQ=255)
gACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaag < 2:1001903/76‑1 (MQ=255)
aaaaGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga < 1:929804/74‑1 (MQ=255)
aCAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga < 1:805810/76‑1 (MQ=255)
aCAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga > 2:624692/1‑76 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa < 2:1666901/76‑1 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa < 1:327321/76‑1 (MQ=255)
cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa < 1:1015719/76‑1 (MQ=255)
aaGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaaa > 2:102659/1‑76 (MQ=255)
aTTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCg > 2:1428266/1‑76 (MQ=255)
|
TTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCG > REL606/3328878‑3329021
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
