breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I
JC	1,733,297	(CGTATGAA)1→2	coding (333/1413 nt)	pykF →	pyruvate kinase
JC	2,103,918	(CCAG)7→8	coding (185/216 nt)	ECB_01992 →	hypothetical protein
RA	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein
RA	2,529,500	T→C	L63P (CTG→CCG)	hyfG →	hydrogenase 4, subunit
JC	3,014,094	(ACAGGC)3→2	coding (675‑680/1137 nt)	ECB_02815 →	Capsule polysaccharide export inner‑membrane protein kpsE
RA	3,328,951	A→G	S10P (TCC→CCC)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB
RA	3,762,741	A→T	K662I (AAA→ATA)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA	3,911,972	(T)7→6	intergenic (‑173/‑150)	yifB ← / → ilvL	predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
MC JC	4,096,207	Δ1,616 bp		[glpK]–glpF	[glpK], glpF
RA	4,163,752	T→G	I966S (ATC→AGC)	rpoB →	DNA‑directed RNA polymerase subunit beta
RA	4,169,030	T→A	I1357N (ATC→AAC)	rpoC →	DNA‑directed RNA polymerase subunit beta'
RA	4,219,546	T→G	I97S (ATT→AGT)	yjbA →	phosphate‑starvation‑inducible protein PsiE
RA	4,411,844	G→T	F130L (TTC→TTA)	ECB_04076 ←	putative oxoacyl reductase
RA	4,438,531	T→G	D110A (GAC→GCC)	fbp ←	fructose‑1,6‑bisphosphatase
JC JC	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	546989–547699	548436	738–1448	13 [11]	[11] 13	[insB‑6]–nmpC	[insB‑6],insA‑6,nmpC
*	*	÷	REL606	548496	558601	10106	13 [12]	[12] 14	ybcR–ECB_00517	ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517
*	*	÷	REL606	561060	569765	8706	13 [12]	[12] 14	envY–[yhhI]	envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,[yhhI]
*	*	÷	REL606	571193	573680	2488	13 [12]	[11] 13	[ECB_00528]–ECB_00530	[ECB_00528],ECB_00529,ECB_00530
*	*	÷	REL606	574114	593359	19246	13 [12]	[11] 13	cusS–[fepA]	cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
*	*	÷	REL606	593581	619823–619116	25536–26243	13 [12]	[12] 14	[fepA]–[insA‑8]	[fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]