breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,174,6480TG35.3% 56.1 / 12.1 35V409G (GTG→GGG) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
*REL6063,492,0820TG35.1% 91.3 / 22.5 57V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6062,296,3290AC33.9% 112.0 / 20.4 56intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6064,574,2520TG28.2% 78.3 / 11.7 39S5S (TCA→TCChpaXhypothetical 4‑hydroxyphenylacetate permease
*REL6061,484,1050AG27.3% 65.8 / 11.9 33G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,882,5260TG25.3% 159.0 / 10.9 79I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6061,747,9030GT23.6% 238.7 / 53.3 89A8S (GCA→TCA) ydiLhypothetical protein

Marginal new junction evidence (lowest skew 10 of 24 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2042274120 (2.120)13 (0.260) 6/134 4.4 12.1% coding (547/1428 nt) wzx O antigen translocase (Wzx)
?REL606 = 2042743 82 (1.620)coding (78/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 3022729130 (2.300)7 (0.130) 6/140 4.6 6.4% intergenic (‑45/+529) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
?REL606 = 3023284 83 (1.570)coding (439/465 nt) ECB_02822 bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
* ? REL606 = 4520817137 (2.420)6 (0.120) 4/134 5.2 5.7% intergenic (‑1178/‑278) yjhA/fimB N‑acetylnuraminic acid outer membrane channel protein/recombinase involved in phase variation; regulator for fimA; b4312_1
?REL606 = 4521290 77 (1.530)pseudogene (196/498 nt) fimB recombinase involved in phase variation; regulator for fimA; b4312_1
* ? REL606 = 3737265145 (2.560)5 (0.100) 4/134 5.2 4.6% coding (562/1254 nt) waaL Lipid A‑core, surface polymer ligase
?REL606 = 3737614 76 (1.510)coding (213/1254 nt) waaL Lipid A‑core, surface polymer ligase
* ? REL606 2039854 =71 (1.250)6 (0.120) 4/134 5.2 7.2% coding (275/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2040304 = 91 (1.800)coding (1080/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 = 203843966 (1.170)5 (0.100) 4/134 5.2 7.6% coding (510/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 = 2038932 63 (1.250)coding (17/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 = 160064787 (1.540)5 (0.100) 4/132 5.2 6.1% intergenic (‑595/‑158) ydfJ/ydfK predicted transporter/predicted DNA‑binding transcriptional regulator
?REL606 = 1601008 NA (NA)coding (204/267 nt) ydfK predicted DNA‑binding transcriptional regulator
* ? REL606 3022392 =102 (1.800)6 (0.120) 4/136 5.3 5.5% coding (293/1140 nt) ECB_02821 hypothetical protein
?REL606 3022865 = 114 (2.220)intergenic (‑181/+393) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
* ? REL606 3021732 =106 (1.870)6 (0.120) 4/138 5.3 5.8% coding (953/1140 nt) ECB_02821 hypothetical protein
?REL606 3022271 = 99 (1.900)coding (414/1140 nt) ECB_02821 hypothetical protein
* ? REL606 = 3021607118 (2.080)5 (0.100) 4/138 5.3 5.1% coding (1078/1140 nt) ECB_02821 hypothetical protein
?REL606 = 3022022 76 (1.460)coding (663/1140 nt) ECB_02821 hypothetical protein