Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A75 F1 I1 R1
|
16 |
50.8 |
3316832 |
95.7% |
3174208 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,911,991 |
(T)5→4 |
intergenic (‑192/‑131) |
yifB ← / → ilvL |
predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,911,987 | 0 | T | . | 88.5%
| 225.0
/ 29.4
| 61 | intergenic (‑188/‑135) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base . (26/28); total (30/31) |
| Fisher's exact test for biased strand distribution p-value = 7.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAC > REL606/3911916‑3912058
|
gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt > 1:1486095/1‑76 (MQ=255)
cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTaaa > 2:1169646/1‑76 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTaaaa < 1:1055833/76‑1 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTaaaa > 2:583846/1‑76 (MQ=255)
aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTAaaaa < 1:1168567/76‑1 (MQ=255)
aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTaaaaaa > 1:126993/1‑76 (MQ=255)
aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTaaaaaa > 1:1407511/1‑76 (MQ=255)
gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAaaca < 1:818548/76‑1 (MQ=255)
tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 1:188373/76‑1 (MQ=255)
gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAacaaca < 1:1482764/76‑1 (MQ=255)
aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa > 1:428616/1‑76 (MQ=255)
aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa < 1:187150/76‑1 (MQ=255)
aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta > 2:34091/1‑76 (MQ=255)
aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta > 2:148280/1‑76 (MQ=255)
aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta > 2:382704/1‑76 (MQ=255)
cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTAACTATTTTA‑TTTTAAAAAACAACAATTtat > 2:229146/1‑76 (MQ=255)
gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtata < 1:86780/76‑1 (MQ=255)
ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATAtt > 2:792246/1‑76 (MQ=255)
gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTg < 2:1459231/76‑1 (MQ=255)
gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTg < 2:543470/76‑1 (MQ=255)
gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTg < 1:1079303/76‑1 (MQ=255)
aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGa < 1:760717/76‑1 (MQ=255)
aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGa < 1:24973/76‑1 (MQ=255)
gggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa < 1:212333/76‑1 (MQ=255)
caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAattatt < 1:261640/76‑1 (MQ=255)
caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAattatt < 2:139001/76‑1 (MQ=255)
caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAattatt < 1:579845/76‑1 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaa > 2:660219/1‑76 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaa > 2:809574/1‑76 (MQ=255)
ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 2:347232/76‑1 (MQ=255)
ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:732612/76‑1 (MQ=255)
ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 2:138989/76‑1 (MQ=255)
cATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca > 1:953284/1‑76 (MQ=255)
aTACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:1484404/1‑76 (MQ=255)
aTACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:1177022/1‑76 (MQ=255)
aTACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:1177012/1‑76 (MQ=255)
tACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 1:223031/1‑76 (MQ=255)
tGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa > 1:786995/1‑76 (MQ=255)
taaaTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa > 2:148402/2‑76 (MQ=255)
aaTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa > 1:417063/1‑76 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa < 1:898945/76‑1 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 2:572534/1‑76 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 2:572529/1‑76 (MQ=255)
tGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc > 2:614248/1‑76 (MQ=255)
gAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 1:504906/1‑76 (MQ=255)
tttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc < 1:1468262/76‑1 (MQ=255)
ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa < 1:1210200/76‑1 (MQ=255)
ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa < 1:287385/76‑1 (MQ=255)
ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa > 1:1318682/1‑76 (MQ=255)
ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa < 1:287401/76‑1 (MQ=255)
tttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaa < 1:1343962/76‑1 (MQ=255)
tttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaa < 1:1567998/76‑1 (MQ=255)
ttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa < 1:981895/76‑1 (MQ=255)
tttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa < 1:1169184/76‑1 (MQ=255)
cactcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat < 1:1030989/76‑1 (MQ=255)
cactcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat > 2:1027482/1‑76 (MQ=255)
actcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAata > 1:1578131/1‑76 (MQ=255)
cactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 2:40673/1‑76 (MQ=255)
actATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 2:233018/1‑76 (MQ=255)
actATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 2:129778/76‑1 (MQ=255)
actATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 2:746092/76‑1 (MQ=255)
ctATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg > 2:1558334/1‑76 (MQ=255)
tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGACCAAAAAATATCTTGt > 1:1635699/1‑76 (MQ=255)
tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGACCAAAAAATATCTTGt > 1:732685/1‑76 (MQ=255)
attttattttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 2:1417917/76‑1 (MQ=255)
atttta‑ttttAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:947176/1‑76 (MQ=255)
tttta‑ttttaAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc < 1:1574004/76‑1 (MQ=255)
tttta‑ttttaAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:1580854/1‑76 (MQ=255)
tttta‑ttttaAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:307567/1‑76 (MQ=255)
|
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAC > REL606/3911916‑3912058
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
