Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A41 F1 I1 R1
|
21 |
57.1 |
3643130 |
97.0% |
3533836 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,203,168 |
+A |
coding (410/3684 nt) |
metH → |
B12‑dependent methionine synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,203,166 | 1 | . | A | 100.0%
| 147.9
/ NA
| 39 | T136T (ACC→ACA) | metH | B12‑dependent methionine synthase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (21/18); total (21/18) |
GCGCCTGTGCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACC‑AACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGG > REL606/4203093‑4203236
|
gcgcCTGTGCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCccaacc‑aa > 1:1585987/1‑76 (MQ=255)
gcCTGTGCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCccaacc‑aaac < 2:1686735/76‑3 (MQ=255)
ccTGTGCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCccaacc‑aaacc > 2:1425134/1‑73 (MQ=255)
ccTGTGCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCccaacc‑aaacc < 1:348160/76‑4 (MQ=255)
ggCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCAc < 1:697522/75‑1 (MQ=255)
cTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACgg > 1:320909/1‑76 (MQ=255)
cTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACgg > 1:833890/1‑76 (MQ=255)
gACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCg < 1:696068/76‑1 (MQ=255)
gAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCt < 1:1496504/76‑1 (MQ=255)
tGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTAtt < 2:854498/76‑1 (MQ=255)
ccGCGCGCACGGCGGAGATACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTtctc < 1:1010872/76‑1 (MQ=255)
ccGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTtctc > 2:352209/1‑76 (MQ=255)
cgcgcgCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTcc < 1:136743/76‑1 (MQ=255)
cgcgcACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCCCACGGCGTCTATTTCTCCgg > 2:1442813/1‑76 (MQ=255)
cACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGt > 1:200921/1‑76 (MQ=255)
cACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGt > 2:1688161/1‑76 (MQ=255)
cACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGt > 2:1660386/1‑76 (MQ=255)
cACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGt > 2:1402492/1‑76 (MQ=255)
aCGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCGCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTc < 2:978908/76‑1 (MQ=255)
cGCCGGAGACACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCa < 2:144091/76‑1 (MQ=255)
gCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCaa < 1:1037306/76‑1 (MQ=255)
cGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACg > 1:464483/1‑76 (MQ=255)
agaAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATc < 2:1092914/76‑1 (MQ=255)
gaAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATcc < 1:1679078/76‑1 (MQ=255)
aaCCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCgg < 2:808608/76‑1 (MQ=255)
aCCGCGCTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGc < 2:1570600/76‑1 (MQ=255)
cTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCg > 2:725584/1‑76 (MQ=255)
cTACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCg > 2:1806325/1‑76 (MQ=255)
tACGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGt < 2:626134/76‑1 (MQ=255)
aCGTTGCCGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTa > 2:5678/1‑76 (MQ=255)
ccGGTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCa < 2:1710542/76‑1 (MQ=255)
ggTGTTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACt < 1:1790972/76‑1 (MQ=255)
ggTGTTCGCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTGAACGATCCGGCATTTCGTAATATCACt < 1:1508659/76‑1 (MQ=255)
gtTCTCGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACtttt > 1:885334/1‑76 (MQ=255)
tctcGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGa > 2:639642/1‑76 (MQ=255)
tctcGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGa > 1:473150/1‑76 (MQ=255)
ctcGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGAc > 1:717555/1‑76 (MQ=255)
tcGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACg > 2:1327990/1‑76 (MQ=255)
tcGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACg < 2:1301271/76‑1 (MQ=255)
cGGCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACgg > 1:624650/1‑76 (MQ=255)
ggCCCAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACggg > 1:681933/1‑76 (MQ=255)
cccAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCt > 1:233117/1‑76 (MQ=255)
cccAACCAAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCt > 1:761630/1‑76 (MQ=255)
caaccaaaCCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCtgg > 2:1553691/1‑76 (MQ=255)
|
GCGCCTGTGCTGACGAGTGGACCGCGCGCACGCCGGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCAACC‑AACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGG > REL606/4203093‑4203236
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
