Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A44 F1 I1 R1
|
19 |
54.5 |
3474856 |
97.7% |
3394934 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,392,351 |
G→T |
A52S (GCA→TCA) |
yfcN → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,392,351 | 0 | G | T | 100.0%
| 112.0
/ NA
| 34 | A52S (GCA→TCA) | yfcN | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/19); total (15/19) |
GGACACGATTGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGGCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAGGCCCGGTGAAA > REL606/2392278‑2392416
|
ggACACGATTGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCa > 1:182906/1‑76 (MQ=255)
aTTGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATgcca < 1:1097252/76‑1 (MQ=255)
ttGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATgccag < 2:812725/76‑1 (MQ=255)
tGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATgccagc > 1:1068845/1‑76 (MQ=255)
tGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATgccagc > 1:407941/1‑76 (MQ=255)
gTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATgccagcc > 2:1001636/1‑76 (MQ=255)
gTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATgccagcc > 1:1359244/1‑76 (MQ=255)
cACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCatt < 2:1396868/76‑1 (MQ=255)
cACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCatt > 2:1344217/1‑76 (MQ=255)
ccgaccgCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCattat < 1:1045316/76‑1 (MQ=255)
cgaccgCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCattatt > 2:467971/1‑76 (MQ=255)
gaccgCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTAttt > 2:1530290/1‑76 (MQ=255)
gaccgCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTAttt > 1:1196894/1‑76 (MQ=255)
cgCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTtctc < 2:606268/76‑1 (MQ=255)
aGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGa > 2:1625201/1‑76 (MQ=255)
gTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGa < 2:1587246/76‑1 (MQ=255)
aGAAAATCAGCGAAGTGCCGGTTAAGGGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGtt < 2:1199289/76‑1 (MQ=255)
aaaaTCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTc < 2:733872/76‑1 (MQ=255)
aaaaTCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTc < 2:1357743/76‑1 (MQ=255)
aaaTCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCa < 1:341051/76‑1 (MQ=255)
cGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTa > 1:737285/1‑76 (MQ=255)
gAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTAt > 1:380919/1‑76 (MQ=255)
gAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTAt < 1:952237/76‑1 (MQ=255)
aaGTGCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTAtt < 1:1305098/76‑1 (MQ=255)
gCCGGTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAAt < 1:625025/76‑1 (MQ=255)
gTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCg < 1:1202857/76‑1 (MQ=255)
gTTAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCg < 2:684752/76‑1 (MQ=255)
ttAAGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGa < 2:331701/76‑1 (MQ=255)
aaGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAg < 2:1485980/76‑1 (MQ=255)
aaGCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAg < 2:1159096/76‑1 (MQ=255)
gCGGTTGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAGGc > 1:1713881/1‑76 (MQ=255)
ttGATTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAGGCCCgg > 2:1077815/1‑76 (MQ=255)
aTTCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAGGCCCGGTGa < 2:796013/76‑1 (MQ=255)
tCAGGAACAGTCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAGGCCCGGTGaaa > 1:1018541/1‑76 (MQ=255)
|
GGACACGATTGTCCACCGACCGCAGCGTAAGAAAATCAGCGAAGTGCCGGTTAAGCGGTTGATTCAGGAACAGGCAGATGCCAGCCATTATTTCTCAGATGAGTTTCAGCCGTTATTGAATACCGAAGGCCCGGTGAAA > REL606/2392278‑2392416
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
