Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A108 F1 I1 R1 80 195.1 12639756 93.3% 11792892 75.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,405,401 (G)6→7 coding (438/1347 nt) fadL → long‑chain fatty acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,405,3951.G99.4% 658.5 / ‑4.4 160V144V (GTC→GTG) fadLlong‑chain fatty acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base G (67/91);  minor base A (1/0);  total (69/91)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 193 total aligned reads displayed.

TGGTTGGGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGA  >  REL606/2405320‑2405465
                                                                             |                                                                        
tggttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑c                                                                           >  2:3867657/1‑76 (MQ=255)
 ggttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑g                                                                        >  1:2023603/1‑76 (MQ=255)
   ttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggg                                                                      <  1:4256131/76‑1 (MQ=255)
   ttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggg                                                                      <  1:2722315/76‑1 (MQ=255)
     ggggCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggggg                                                                    <  1:5136025/76‑1 (MQ=255)
       ggCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggggggg                                                                  <  2:1462947/76‑2 (MQ=255)
       ggCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggggggg                                                                  <  2:2657924/76‑2 (MQ=255)
         cgcTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑gggggggac                                                                <  1:749100/76‑4 (MQ=255)
            ttCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAAc                                                              <  2:1631091/76‑1 (MQ=255)
               tATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGa                                                           >  2:2577158/1‑76 (MQ=255)
                aTTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGAc                                                          >  1:6268451/1‑76 (MQ=255)
                aTTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑GGGGGGGAAACCGAcc                                                         >  1:5383159/1‑76 (MQ=255)
                 ttACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGAcc                                                         >  1:3656702/1‑76 (MQ=255)
                   aCCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCtt                                                       <  2:4880054/76‑1 (MQ=255)
                   aCCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCCACCtt                                                       >  2:2015694/1‑76 (MQ=255)
                   aCCACTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCtt                                                       <  1:241461/76‑1 (MQ=255)
                     ctctAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGTCC‑‑GGGGGGACCACCGCCCCTG‑a                                                    >  2:3016587/1‑76 (MQ=255)
                     ctctAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑a                                                    <  2:1706765/76‑1 (MQ=255)
                        tAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAAc                                                 >  2:2251975/1‑76 (MQ=255)
                        tAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAAc                                                 >  1:5722135/1‑76 (MQ=255)
                         aaCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAAcc                                                >  1:1700887/1‑76 (MQ=255)
                         aaCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAAcc                                                >  1:3255241/1‑76 (MQ=255)
                         aaCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAAcc                                                >  1:2798868/1‑76 (MQ=255)
                          aCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCa                                               <  1:4217529/76‑1 (MQ=255)
                           cTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAACCAt                                              >  1:5045201/1‑76 (MQ=255)
                           cTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCAt                                              <  1:1539354/76‑1 (MQ=255)
                           cTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCCTG‑AAAACAt                                              >  2:3440593/1‑76 (MQ=255)
                            tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAACCATg                                             >  1:293391/1‑76 (MQ=255)
                            tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAAACGACCTTG‑AAACCATg                                             >  2:466407/1‑76 (MQ=255)
                            tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATg                                             >  1:2637580/1‑76 (MQ=255)
                            tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATg                                             >  1:2379440/1‑76 (MQ=255)
                             aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa                                            <  2:4395037/76‑1 (MQ=255)
                             aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa                                            <  1:5808444/76‑1 (MQ=255)
                             aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa                                            >  1:1865541/1‑76 (MQ=255)
                             aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa                                            <  1:2906989/76‑1 (MQ=255)
                              tGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGaa                                           <  2:1068067/76‑1 (MQ=255)
                               ggTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAATCCACCTTGCAAACCATTaa                                           >  2:2693189/1‑76 (MQ=255)
                               ggTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAAc                                          <  1:4029003/76‑1 (MQ=255)
                                gTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CGGGGGGGGACAACCTACCTTG‑AAACCATGAAc                                          >  1:5022053/1‑76 (MQ=255)
                                gTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGTCCTTG‑AAACCCTGAAcc                                         >  1:1848510/1‑76 (MQ=255)
                                 tCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCt                                        <  2:3681227/76‑1 (MQ=255)
                                 tCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCt                                        <  1:6015812/76‑1 (MQ=255)
                                  cTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTg                                       <  2:4456622/76‑1 (MQ=255)
                                   tGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGa                                      <  1:2509079/76‑1 (MQ=255)
                                   tGGCTACAGAGTGTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGa                                      <  2:1238171/76‑1 (MQ=255)
                                    ggCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGaa                                     <  2:3637289/76‑1 (MQ=255)
                                    ggCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGaa                                     <  2:4147156/76‑1 (MQ=255)
                                     gCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAAc                                    <  1:3686699/76‑1 (MQ=255)
                                      cTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGAACTTG‑AAACCATGAACCTGAACt                                   >  2:1699039/1‑76 (MQ=255)
                                       tACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACtt                                  <  1:6290953/76‑1 (MQ=255)
                                         cAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTaa                                <  2:1474672/76‑1 (MQ=255)
                                         cAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTaa                                <  1:4965815/76‑1 (MQ=255)
                                         cAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTaa                                <  2:3077936/76‑1 (MQ=255)
                                          agagTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAg                               >  2:399520/1‑76 (MQ=255)
                                            agTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCg                             >  1:2479862/1‑76 (MQ=255)
                                                tAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGc                         <  1:549735/76‑1 (MQ=255)
                                                 aaCGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCg                        <  2:1286378/76‑1 (MQ=255)
                                                  aCGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGt                       >  1:1394022/1‑76 (MQ=255)
                                                  aCGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGt                       >  2:5805662/1‑76 (MQ=255)
                                                   cGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTa                      >  1:1213427/1‑76 (MQ=255)
                                                   cGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTa                      >  1:1140480/1‑76 (MQ=255)
                                                   cGATACTTATGCAGGAGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTa                      <  1:2989622/76‑1 (MQ=255)
                                                    gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCCTGAACCTGAACTTAAGCGGTGCGTAt                     >  2:4811507/1‑76 (MQ=255)
                                                    gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt                     <  1:2633487/76‑1 (MQ=255)
                                                    gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt                     <  2:3136728/76‑1 (MQ=255)
                                                    gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt                     <  2:5827366/76‑1 (MQ=255)
                                                    gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt                     <  1:4602303/76‑1 (MQ=255)
                                                     aTACTTATGCAGGCGGCTCTGT‑CGGGGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt                     <  1:5299905/76‑1 (MQ=255)
                                                     aTACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATc                    >  1:485982/1‑76 (MQ=255)
                                                     aTACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAAACCACACTG‑AAACCACGAACCCGAAATCAAGCGGTGCGTGTc                    >  2:3667603/1‑76 (MQ=255)
                                                      tACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCg                   <  1:960669/76‑1 (MQ=255)
                                                      tACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCg                   <  2:1366824/76‑1 (MQ=255)
                                                         ttATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCtt                <  2:2443539/76‑1 (MQ=255)
                                                         ttATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCtt                <  2:5114414/76‑1 (MQ=255)
                                                          tATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTa               <  2:734436/76‑1 (MQ=255)
                                                           aTGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaa              <  1:2302053/76‑1 (MQ=255)
                                                           aTGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaa              <  1:2513973/76‑1 (MQ=255)
                                                            tGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaaa             <  1:4002457/76‑1 (MQ=255)
                                                            tGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaaa             >  1:2575211/1‑76 (MQ=255)
                                                             gCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaat            <  1:4331316/76‑1 (MQ=255)
                                                              cAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata           >  2:6263536/1‑76 (MQ=255)
                                                              cAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata           <  2:775915/76‑1 (MQ=255)
                                                              cAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata           >  2:1129585/1‑76 (MQ=255)
                                                               aGGCGGCTCTGT‑CGGGGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata           <  2:5246981/76‑1 (MQ=255)
                                                               aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa          <  2:4605781/76‑1 (MQ=255)
                                                               aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa          >  2:47392/1‑76 (MQ=255)
                                                               aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa          <  1:2162690/76‑1 (MQ=255)
                                                               aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa          >  2:4482683/1‑76 (MQ=255)
                                                               aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa          >  1:4116805/1‑76 (MQ=255)
                                                                ggcggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataat         <  1:3463589/76‑1 (MQ=255)
                                                                ggcggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataat         <  2:6077604/76‑1 (MQ=255)
                                                                   ggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCa      <  2:1928129/76‑1 (MQ=255)
                                                                   ggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCa      >  1:6143408/1‑76 (MQ=255)
                                                                   ggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCa      <  1:4472912/76‑1 (MQ=255)
                                                                    gcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCAt     <  2:557707/76‑1 (MQ=255)
                                                                      tctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATgg   <  1:874686/76‑1 (MQ=255)
                                                                      tctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATgg   <  2:887294/76‑1 (MQ=255)
                                                                       ctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGa  <  2:3485018/76‑1 (MQ=255)
                                                                       ctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGa  >  2:6299475/1‑76 (MQ=255)
                                                                       ctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGa  >  1:2961666/1‑76 (MQ=255)
                                                                             |                                                                        
TGGTTGGGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGA  >  REL606/2405320‑2405465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A