breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 66,630 A→G L652P (CTA→CCA)  polB ← DNA polymerase II
RA 79,133 (T)5→4 coding (971/1656 nt) yabN ← DNA‑binding transcriptional regulator
RA 242,203 (G)8→9 coding (193/372 nt) ECB_00212 → hypothetical protein
RA 283,483 T→C intergenic (‑526/‑1054) ykgM ← / → eaeH 50S ribosomal protein L31/attaching and effacing protein, pathogenesis factor
RA 340,408 (GC)6→5 coding (682‑683/948 nt) mhpR ← DNA‑binding transcriptional activator, 3HPP‑binding
RA 352,377 (G)8→7 intergenic (‑139/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/hypothetical protein
JC 422,600 (G)7→10 intergenic (‑287/+175) cyoA ← / ← ampG cytochrome o ubiquinol oxidase subunit II/muropeptide transporter
RA 474,010 (C)5→6 coding (1296/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 539,303 A→G C97R (TGC→CGC)  ECB_00490 ← putative DNA N‑6‑adenine‑methyltransferase of bacteriophage
RA 576,685 T→C N61N (AAT→AAC cusC → copper/silver efflux system, outer membrane component
RA 649,391 T→G I471L (ATC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 670,789 A→G A452A (GCA→GCG ECB_00624 → conserved hypothetical protein
RA 782,538 A→G K8R (AAG→AGG)  ybhI → predicted transporter
RA 783,336 T→A L274Q (CTG→CAG)  ybhI → predicted transporter
RA 805,738 C→T intergenic (+165/‑414) bioD → / → uvrB dethiobiotin synthetase/excinuclease ABC subunit B
RA 837,880 (G)5→6 coding (226/2226 nt) ybiO ← predicted mechanosensitive channel
RA 852,548 G→A D11D (GAC→GAT ybiV ← predicted hydrolase
RA 870,805 C→T F2F (TTC→TTT yliH → hypothetical protein
RA 939,359 (G)5→6 intergenic (‑63/‑260) cspD ← / → clpS cold shock protein homolog/ATP‑dependent Clp protease adaptor protein ClpS
RA 1,002,938 (A)8→7 intergenic (‑137/+48) aspC ← / ← ompF aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F)
RA 1,030,403 C→T P18P (CCC→CCT pqiB → paraquat‑inducible protein B
RA 1,111,966 (G)7→8 intergenic (‑235/+167) insE‑1 ← / ← serX putative transposase‑related protein/tRNA‑Ser
RA 1,146,395 C→T T129I (ACC→ATC)  flgC → flagellar basal‑body rod protein C
RA 1,160,663 C→T D168N (GAT→AAT)  maf ← Maf‑like protein
RA 1,183,400 (T)8→7 coding (27/633 nt) ycfQ ← predicted DNA‑binding transcriptional regulator
RA 1,198,004 A→G Y66H (TAT→CAT)  potD ← spermidine/putrescine ABC transporter periplasmic substrate‑binding protein
RA 1,282,843 C→T P944S (CCG→TCG)  narG → nitrate reductase 1, alpha subunit
RA 1,296,053 (T)5→4 coding (862/2676 nt) adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
JC 1,306,026 Δ10 bp coding (205‑214/1461 nt) cls ← cardiolipin synthetase
RA 1,322,724 G→A pseudogene (385/408 nt) yciQ → hypothetical protein; b1268_1
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,360,063 A→G P192P (CCA→CCG ycjL → gamma‑Glu‑GABA hydrolase
RA 1,440,659 T→C intergenic (+35/+24) feaB → / ← tynA phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring
RA 1,448,469 A→G intergenic (+13/‑3) ynbB → / → ynbC predicted CDP‑diglyceride synthase/predicted hydrolase
RA 1,480,447 T→C F112S (TTT→TCT)  ydcR → fused predicted DNA‑binding transcriptional regulator/predicted amino transferase
RA 1,489,606 C→T A157V (GCG→GTG)  yncB → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
RA 1,504,870 T→C S129S (AGT→AGC ydcD → hypothetical protein
RA 1,505,141 A→G intergenic (+175/‑7) ydcD → / → ECB_01416 hypothetical protein/hypothetical protein
RA 1,512,877 T→C N43S (AAC→AGC)  narY ← nitrate reductase 2 (NRZ), beta subunit
RA 1,602,152 2 bp→AT intergenic (+175/+52) pinQ → / ← ECB_01505 predicted site‑specific recombinase/conserved hypothetical protein
RA 1,691,520 (T)9→8 intergenic (+55/‑51) ydgR → / → gst putative tripeptide transporter permease/glutathionine S‑transferase
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,895,222 (C)6→7 coding (388/1284 nt) yebS → conserved inner membrane protein
RA 1,936,315 G→A G196G (GGC→GGT torY ← TMAO reductase III (TorYZ), cytochrome c‑type subunit
RA 2,076,609 G→A T46T (ACG→ACA yegE → predicted diguanylate cyclase, GGDEF domain signalling protein
RA 2,131,466 (A)9→8 intergenic (‑125/+175) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA 2,173,730 G→A R103H (CGT→CAT)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,405,401 (G)6→7 coding (438/1347 nt) fadL → long‑chain fatty acid outer membrane transporter
RA 2,577,214 T→C intergenic (‑37/+21) pepB ← / ← yfhJ aminopeptidase B/hypothetical protein
RA 2,708,572 G→A W366* (TGG→TGA emrB → multidrug efflux system protein
RA 2,776,287 A→G V176A (GTA→GCA)  cysH ← phosphoadenosine phosphosulfate reductase
RA 3,011,220 T→C intergenic (+191/+47) ECB_02811 → / ← ECB_02812 conserved hypothetical protein/conserved hypothetical protein
RA 3,142,600 G→A A254V (GCG→GTG)  ygjD ← O‑sialoglycoprotein endopeptidase
RA 3,145,830 A→G intergenic (+161/‑34) dnaG → / → rpoD DNA primase/RNA polymerase sigma factor
RA 3,340,143 G→A P200P (CCG→CCA yhdJ → predicted methyltransferase
RA 3,489,214 T→A intergenic (‑8/+9) glpR ← / ← glpG DNA‑binding transcriptional repressor/predicted intramembrane serine protease
RA 3,498,010 G→A S9L (TCG→TTG)  glgP ← glycogen phosphorylase
RA 3,517,709 T→C Y31H (TAT→CAT)  yhhA → hypothetical protein
RA 3,529,506 C→T A280T (GCG→ACG)  livJ ← leucine/isoleucine/valine transporter subunit
RA 3,589,641 G→A E307K (GAA→AAA)  yhiU → multidrug resistance efflux transporter
RA 3,751,297 C→T A71V (GCC→GTC)  dfp → bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,972 (T)7→6 intergenic (‑173/‑150) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 3,912,276 (T)8→7 intergenic (+56/‑84) ilvL → / → ilvG ilvG operon leader peptide/acetolactate synthase II, valine insensitive, large subunit
RA 3,944,574 C→T noncoding (40/77 nt) proM → tRNA‑Pro
RA 3,985,084 A→G V437A (GTA→GCA)  ECB_03714 ← conserved hypothetical protein
RA 4,038,538 (T)9→8 intergenic (+50/‑167) bipA → / → yihL GTP‑binding protein/predicted DNA‑binding transcriptional regulator
RA 4,164,097 C→T P1081L (CCG→CTG)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,164,802 A→G E1316G (GAG→GGG)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,278,072 A→G G39G (GGT→GGC fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit
RA 4,302,867 A→T D100E (GAT→GAA phnD ← phosphonate/organophosphate ester transporter subunit
JC 4,375,781 (TGGCGC)3→2 coding (215‑220/1848 nt) mutL → DNA mismatch repair protein
RA 4,402,611 (A)7→8 intergenic (+70/+59) sgaE → / ← yjfY L‑ribulose 5‑phosphate 4‑epimerase/hypothetical protein
RA 4,523,210 C→T T87I (ACC→ATC)  fimE → tyrosine recombinase/inversion of on/off regulator of fimA
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)
RA 4,595,328 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,608,514 T→C V351A (GTC→GCC)  deoA → thymidine phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3549983–3551293 3552273–3551398 106–2291 87 [86] [85] 87 rhsB rhsB element core protein RshB