breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 66,630 | A→G | L652P (CTA→CCA) | polB ← | DNA polymerase II |
RA | 79,133 | (T)5→4 | coding (971/1656 nt) | yabN ← | DNA‑binding transcriptional regulator |
RA | 242,203 | (G)8→9 | coding (193/372 nt) | ECB_00212 → | hypothetical protein |
RA | 283,483 | T→C | intergenic (‑526/‑1054) | ykgM ← / → eaeH | 50S ribosomal protein L31/attaching and effacing protein, pathogenesis factor |
RA | 340,408 | (GC)6→5 | coding (682‑683/948 nt) | mhpR ← | DNA‑binding transcriptional activator, 3HPP‑binding |
RA | 352,377 | (G)8→7 | intergenic (‑139/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/hypothetical protein |
JC | 422,600 | (G)7→10 | intergenic (‑287/+175) | cyoA ← / ← ampG | cytochrome o ubiquinol oxidase subunit II/muropeptide transporter |
RA | 474,010 | (C)5→6 | coding (1296/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 539,303 | A→G | C97R (TGC→CGC) | ECB_00490 ← | putative DNA N‑6‑adenine‑methyltransferase of bacteriophage |
RA | 576,685 | T→C | N61N (AAT→AAC) | cusC → | copper/silver efflux system, outer membrane component |
RA | 649,391 | T→G | I471L (ATC→CTC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 670,789 | A→G | A452A (GCA→GCG) | ECB_00624 → | conserved hypothetical protein |
RA | 782,538 | A→G | K8R (AAG→AGG) | ybhI → | predicted transporter |
RA | 783,336 | T→A | L274Q (CTG→CAG) | ybhI → | predicted transporter |
RA | 805,738 | C→T | intergenic (+165/‑414) | bioD → / → uvrB | dethiobiotin synthetase/excinuclease ABC subunit B |
RA | 837,880 | (G)5→6 | coding (226/2226 nt) | ybiO ← | predicted mechanosensitive channel |
RA | 852,548 | G→A | D11D (GAC→GAT) | ybiV ← | predicted hydrolase |
RA | 870,805 | C→T | F2F (TTC→TTT) | yliH → | hypothetical protein |
RA | 939,359 | (G)5→6 | intergenic (‑63/‑260) | cspD ← / → clpS | cold shock protein homolog/ATP‑dependent Clp protease adaptor protein ClpS |
RA | 1,002,938 | (A)8→7 | intergenic (‑137/+48) | aspC ← / ← ompF | aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F) |
RA | 1,030,403 | C→T | P18P (CCC→CCT) | pqiB → | paraquat‑inducible protein B |
RA | 1,111,966 | (G)7→8 | intergenic (‑235/+167) | insE‑1 ← / ← serX | putative transposase‑related protein/tRNA‑Ser |
RA | 1,146,395 | C→T | T129I (ACC→ATC) | flgC → | flagellar basal‑body rod protein C |
RA | 1,160,663 | C→T | D168N (GAT→AAT) | maf ← | Maf‑like protein |
RA | 1,183,400 | (T)8→7 | coding (27/633 nt) | ycfQ ← | predicted DNA‑binding transcriptional regulator |
RA | 1,198,004 | A→G | Y66H (TAT→CAT) | potD ← | spermidine/putrescine ABC transporter periplasmic substrate‑binding protein |
RA | 1,282,843 | C→T | P944S (CCG→TCG) | narG → | nitrate reductase 1, alpha subunit |
RA | 1,296,053 | (T)5→4 | coding (862/2676 nt) | adhE ← | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase |
JC | 1,306,026 | Δ10 bp | coding (205‑214/1461 nt) | cls ← | cardiolipin synthetase |
RA | 1,322,724 | G→A | pseudogene (385/408 nt) | yciQ → | hypothetical protein; b1268_1 |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
RA | 1,360,063 | A→G | P192P (CCA→CCG) | ycjL → | gamma‑Glu‑GABA hydrolase |
RA | 1,440,659 | T→C | intergenic (+35/+24) | feaB → / ← tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
RA | 1,448,469 | A→G | intergenic (+13/‑3) | ynbB → / → ynbC | predicted CDP‑diglyceride synthase/predicted hydrolase |
RA | 1,480,447 | T→C | F112S (TTT→TCT) | ydcR → | fused predicted DNA‑binding transcriptional regulator/predicted amino transferase |
RA | 1,489,606 | C→T | A157V (GCG→GTG) | yncB → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
RA | 1,504,870 | T→C | S129S (AGT→AGC) | ydcD → | hypothetical protein |
RA | 1,505,141 | A→G | intergenic (+175/‑7) | ydcD → / → ECB_01416 | hypothetical protein/hypothetical protein |
RA | 1,512,877 | T→C | N43S (AAC→AGC) | narY ← | nitrate reductase 2 (NRZ), beta subunit |
RA | 1,602,152 | 2 bp→AT | intergenic (+175/+52) | pinQ → / ← ECB_01505 | predicted site‑specific recombinase/conserved hypothetical protein |
RA | 1,691,520 | (T)9→8 | intergenic (+55/‑51) | ydgR → / → gst | putative tripeptide transporter permease/glutathionine S‑transferase |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 1,895,222 | (C)6→7 | coding (388/1284 nt) | yebS → | conserved inner membrane protein |
RA | 1,936,315 | G→A | G196G (GGC→GGT) | torY ← | TMAO reductase III (TorYZ), cytochrome c‑type subunit |
RA | 2,076,609 | G→A | T46T (ACG→ACA) | yegE → | predicted diguanylate cyclase, GGDEF domain signalling protein |
RA | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
RA | 2,173,730 | G→A | R103H (CGT→CAT) | dld → | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 2,405,401 | (G)6→7 | coding (438/1347 nt) | fadL → | long‑chain fatty acid outer membrane transporter |
RA | 2,577,214 | T→C | intergenic (‑37/+21) | pepB ← / ← yfhJ | aminopeptidase B/hypothetical protein |
RA | 2,708,572 | G→A | W366* (TGG→TGA) | emrB → | multidrug efflux system protein |
RA | 2,776,287 | A→G | V176A (GTA→GCA) | cysH ← | phosphoadenosine phosphosulfate reductase |
RA | 3,011,220 | T→C | intergenic (+191/+47) | ECB_02811 → / ← ECB_02812 | conserved hypothetical protein/conserved hypothetical protein |
RA | 3,142,600 | G→A | A254V (GCG→GTG) | ygjD ← | O‑sialoglycoprotein endopeptidase |
RA | 3,145,830 | A→G | intergenic (+161/‑34) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
RA | 3,340,143 | G→A | P200P (CCG→CCA) | yhdJ → | predicted methyltransferase |
RA | 3,489,214 | T→A | intergenic (‑8/+9) | glpR ← / ← glpG | DNA‑binding transcriptional repressor/predicted intramembrane serine protease |
RA | 3,498,010 | G→A | S9L (TCG→TTG) | glgP ← | glycogen phosphorylase |
RA | 3,517,709 | T→C | Y31H (TAT→CAT) | yhhA → | hypothetical protein |
RA | 3,529,506 | C→T | A280T (GCG→ACG) | livJ ← | leucine/isoleucine/valine transporter subunit |
RA | 3,589,641 | G→A | E307K (GAA→AAA) | yhiU → | multidrug resistance efflux transporter |
RA | 3,751,297 | C→T | A71V (GCC→GTC) | dfp → | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,972 | (T)7→6 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 3,912,276 | (T)8→7 | intergenic (+56/‑84) | ilvL → / → ilvG | ilvG operon leader peptide/acetolactate synthase II, valine insensitive, large subunit |
RA | 3,944,574 | C→T | noncoding (40/77 nt) | proM → | tRNA‑Pro |
RA | 3,985,084 | A→G | V437A (GTA→GCA) | ECB_03714 ← | conserved hypothetical protein |
RA | 4,038,538 | (T)9→8 | intergenic (+50/‑167) | bipA → / → yihL | GTP‑binding protein/predicted DNA‑binding transcriptional regulator |
RA | 4,164,097 | C→T | P1081L (CCG→CTG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,164,802 | A→G | E1316G (GAG→GGG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,278,072 | A→G | G39G (GGT→GGC) | fdhF ← | formate dehydrogenase‑H, selenopolypeptide subunit |
RA | 4,302,867 | A→T | D100E (GAT→GAA) | phnD ← | phosphonate/organophosphate ester transporter subunit |
JC | 4,375,781 | (TGGCGC)3→2 | coding (215‑220/1848 nt) | mutL → | DNA mismatch repair protein |
RA | 4,402,611 | (A)7→8 | intergenic (+70/+59) | sgaE → / ← yjfY | L‑ribulose 5‑phosphate 4‑epimerase/hypothetical protein |
RA | 4,523,210 | C→T | T87I (ACC→ATC) | fimE → | tyrosine recombinase/inversion of on/off regulator of fimA |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
RA | 4,595,328 | (G)8→9 | noncoding (72/87 nt) | leuV ← | tRNA‑Leu |
RA | 4,608,514 | T→C | V351A (GTC→GCC) | deoA → | thymidine phosphorylase |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 3549983–3551293 | 3552273–3551398 | 106–2291 | 87 [86] | [85] 87 | rhsB | rhsB element core protein RshB |